pLV2-TAB1-3×HA-Puro plasmid

pLV2-TAB1-3×HA-Puro plasmid

PVT92864

pLV2-TAB1-3×HA-Puro plasmid Information

Plasmid name: pLV2-TAB1(human)-3×HA-Puro
Alias: NM_006116.3;3'-Tab1; MAP3K7IP1
Gene lenght: 1512bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-TAB1-3×HA-Puro plasmid Description

pLV2-TAB1-3×HA-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-TAB1-3×HA-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatggcggc gcagaggagg agcttgctgc agagtgagca 2881 gcagccaagc tggacagatg acctgcctct ctgccacctc tctggggttg gctcagcctc 2941 caaccgcagc tactctgctg atggcaaggg cactgagagc cacccgccag aggacagctg 3001 gctcaagttc aggagtgaga acaactgctt cctgtatggg gtcttcaacg gctatgatgg 3061 caaccgagtg accaacttcg tggcccagcg gctgtccgca gagctcctgc tgggccagct 3121 gaatgccgag cacgccgagg ccgatgtgcg gcgtgtgctg ctgcaggcct tcgatgtggt 3181 ggagaggagc ttcctggagt ccattgacga cgccttggct gagaaggcaa gcctccagtc 3241 gcaattgcca gagggagtcc ctcagcacca gctgcctcct cagtatcaga agatccttga 3301 gagactcaag acgttagaga gggaaatttc gggaggggcc atggccgttg tggcggtcct 3361 tctcaacaac aagctctacg tcgccaatgt cggtacaaac cgtgcacttt tatgcaaatc 3421 gacagtggat gggttgcagg tgacacagct gaacgtggac cacaccacag agaacgagga 3481 tgagctcttc cgtctttcgc agctgggctt ggatgctgga aagatcaagc aggtggggat 3541 catctgtggg caggagagca cccggcggat cggggattac aaggttaaat atggctacac 3601 ggacattgac cttctcagcg ctgccaagtc caaaccaatc atcgcagagc cagaaatcca 3661 tggggcacag ccgctggatg gggtgacggg cttcttggtg ctgatgtcgg aggggttgta 3721 caaggcccta gaggcagccc atgggcctgg gcaggccaac caggagattg ctgcgatgat 3781 tgacactgag tttgccaagc agacctccct ggacgcagtg gcccaggccg tcgtggaccg 3841 ggtgaagcgc atccacagcg acaccttcgc cagtggtggg gagcgtgcca ggttctgccc 3901 ccggcacgag gacatgaccc tgctagtgag gaactttggc tacccgctgg gcgaaatgag 3961 ccagcccaca ccgagcccag ccccagctgc aggaggacga gtgtaccctg tgtctgtgcc 4021 atactccagc gcccagagca ccagcaagac cagcgtgacc ctctcccttg tcatgccctc 4081 ccagggccag atggtcaacg gggctcacag tgcttccacc ctggacgaag ccacccccac 4141 cctcaccaac caaagcccga ccttaaccct gcagtccacc aacacgcaca cgcagagcag 4201 cagctccagc tctgacggag gcctcttccg ctcccggccc gcccactcgc tcccgcctgg 4261 cgaggacggt cgtgttgagc cctatgtgga ctttgctgag ttttaccgcc tctggagcgt 4321 ggaccatggc gagcagagcg tggtgacagc accgggatcc TACCCATACG ACGTACCTGA 4381 TTATGCAACT TATCCTTATG ATGTACCTGA TTATGCTAGC TATCCTTATG ATGTCCCAGA 4441 CTACGCATAG tctagataat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg 4501 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg 4561 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc 4621 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg 4681 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc 4741 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc 4801 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag 4861 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg 4921 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag 4981 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg 5041 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat 5101 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 5161 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 5221 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 5281 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 5341 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 5401 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 5461 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 5521 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 5581 gcctgaccgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg 5641 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 5701 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 5761 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 5821 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 5881 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 5941 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac 6001 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 6061 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 6121 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 6181 ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt 6241 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag 6301 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta 6361 gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga 6421 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag 6481 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta 6541 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta 6601 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt 6661 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac 6721 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc 6781 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc 6841 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 6901 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 6961 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 7021 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 7081 aatatcagag agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga 7141 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 7201 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 7261 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 7321 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 7381 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 7441 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 7501 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 7561 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 7621 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 7681 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 7741 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 7801 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 7861 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 7921 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 7981 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 8041 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 8101 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 8161 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 8221 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 8281 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 8341 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 8401 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 8461 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 8521 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 8581 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 8641 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 8701 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 8761 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 8821 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 8881 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 8941 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 9001 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 9061 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 9121 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 9181 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 9241 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 9301 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac 9361 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 9421 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa 9481 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg 9541 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt 9601 atttgttaaa tatgtactac aaacttagta gtttttaaag aaattgtatt tgttaaatat 9661 gtactacaaa cttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.