pLV2-CMV-NUAK1-3×FLAG-Puro pla

pLV2-CMV-NUAK1-3×FLAG-Puro plasmid

PVT92801

pLV2-CMV-NUAK1-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-CMV-NUAK1(human)-3×FLAG-Puro
Alias: NM_014840.3;ARK5
Gene lenght: 1983bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-NUAK1-3×FLAG-Puro plasmid Description

pLV2-CMV-NUAK1-3×FLAG-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-CMV-NUAK1-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatggaagg ggccgccgcg cctgtggcgg gggaccgccc 2881 cgacttgggg ctgggggcgc cgggctctcc ccgagaggcg gtggcggggg cgactgcagc 2941 cctggagccc aggaagccgc acggggtgaa gcggcatcac cacaagcaca acttgaagca 3001 ccgctacgag ctgcaggaga ccctgggcaa aggcacctac ggcaaagtca agcgggccac 3061 cgagaggttt tctggccgag tggttgctat aaaatccatt cgtaaggaca aaattaagga 3121 tgaacaagac atggttcaca tcagacgaga gattgagatc atgtcatctc tcaaccatcc 3181 tcatatcatc agtatttatg aagtgtttga gaacaaagat aagattgtga tcatcatgga 3241 atatgccagc aaaggggagc tgtacgatta catcagtgag cggcgacgcc tcagtgagag 3301 ggagacccgg cacttcttcc ggcagatcgt ctctgctgtg cactattgtc acaagaacgg 3361 tgtggtccac cgggacttga agctggaaaa tatactgctc gatgacaact gcaatattaa 3421 gattgctgac tttgggcttt ccaacctgta ccagaaggat aagttcttac aaacgttttg 3481 tgggagtcca ctctatgcat ctcctgagat tgtcaatggg agaccttacc gagggccaga 3541 ggtggacagc tgggccctgg gtgtgttgct ttacactctt gtttatggaa caatgccctt 3601 cgatggtttc gatcacaaaa acctcattcg gcaaatcagc agcggagagt accgggagcc 3661 aacacagccc tcagatgctc gaggactcat acggtggatg ctgatggtga accccgatcg 3721 ccgggccact attgaggaca ttgccaacca ctggtgggtg aactggggct ataagagcag 3781 cgtgtgtgac tgtgatgccc tccatgactc tgagtcccca ctcctggctc ggatcattga 3841 ctggcaccac cgttccacag ggctgcaggc tgacaccgaa gccaaaatga agggcctggc 3901 caaacccacg acctctgagg tcatgctaga gcggcagcgg tcgctgaaga aatccaagaa 3961 agagaatgac tttgctcagt ctggtcagga tgcagtgcct gaaagcccat ccaagttgag 4021 ttctaagagg cccaagggga tcctgaagaa gcgaagcaac agcgagcatc gctctcacag 4081 cactggcttc attgaaggtg tagttggtcc tgccttaccc tctactttca agatggagca 4141 ggacttgtgc aggactggcg tgctcctccc aagctcacca gaggcagagg tgccgggaaa 4201 actcagcccc aagcagtcgg ccacgatgcc caagaaaggc atcttgaaaa agacccagca 4261 gagagaatca ggttactact cttccccaga gcgcagtgag tcttcggagc tgttggacag 4321 taatgatgtg atgggcagca gcatcccctc ccccagcccc ccggacccag ccagggtaac 4381 ctcccacagc ctctcctgcc ggaggaaggg catcttgaaa cacagcagca aatactcagc 4441 gggcaccatg gacccagccc tggtcagccc tgaaatgccc acactggaat ccctgtcaga 4501 gcctggtgtc cctgccgagg gcctctcccg gagctacagc cgcccttcca gtgtcatcag 4561 cgatgacagc gtgctgtcca gcgactcttt tgacttgctg gatttgcagg agaatcgccc 4621 tgcccgccag cgcatccgca gctgcgtctc tgcagaaaac ttcctccaga tccaggactt 4681 tgaggggctc cagaaccggc cccggcccca gtacctgaag cggtaccgga accggctggc 4741 agacagcagc ttctccctcc tcacagacat ggatgatgtg actcaggtct acaagcaagc 4801 gctggagatc tgcagcaagc tcaacggatc cGACTACAAA GACCATGACG GTGATTATAA 4861 AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGtag tctagataat tctaccgggt 4921 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac 4981 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca 5041 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa 5101 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct 5161 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg 5221 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg 5281 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg 5341 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc 5401 atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg 5461 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc 5521 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag 5581 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 5641 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 5701 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 5761 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 5821 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 5881 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 5941 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 6001 cgcacctggt gcatgacccg caagcccggt gcctgaccgc gtctggaaca atcaacctct 6061 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 6121 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 6181 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 6241 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 6301 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 6361 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 6421 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc 6481 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 6541 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 6601 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga 6661 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt 6721 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct 6781 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg 6841 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac 6901 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat 6961 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag 7021 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac 7081 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc 7141 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt 7201 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc 7261 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat 7321 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 7381 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 7441 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 7501 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg 7561 ctagcgtttt accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc 7621 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 7681 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 7741 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 7801 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 7861 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 7921 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 7981 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 8041 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 8101 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 8161 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 8221 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 8281 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 8341 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 8401 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 8461 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 8521 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 8581 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 8641 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 8701 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 8761 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 8821 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 8881 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 8941 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 9001 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 9061 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 9121 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 9181 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 9241 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 9301 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 9361 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 9421 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 9481 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 9541 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 9601 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 9661 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 9721 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc 9781 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 9841 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 9901 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc 9961 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc 10021 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta 10081 gtttttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.