pRR-PGK-mCherry-Myc plasmid

pRR-PGK-mCherry-Myc plasmid

PVT92737

pRR-PGK-mCherry-Myc plasmid Information

Plasmid name: pRR-PGK-mCherry-Myc
Alias:
Gene lenght: 966bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Non
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Red
Promoter: hPGK
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: hPGK-F
Reverse primer:
Copy number: High
Induction:

pRR-PGK-mCherry-Myc plasmid Description

pRR-PGK-mCherry-Myc is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pRR-PGK-mCherry-Myc plasmid Sequence

1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat 1801 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta 1861 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa 1921 aattttatcg atcacgagac tagcctcgag aagcttgata tcgaattcca cggggttggg 1981 gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg 2041 ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag 2101 cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc 2161 cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc 2221 acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc 2281 gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga 2341 aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg 2401 gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg 2461 aatcaccgac ctctctcccc agggggatcc GATCCATGTG GTGGCGCCTG TGGTGGCTGC 2521 TGCTGCTGCT GCTGCTGCTG TGGCCCATGG TGTGGGCCaa gcttTACGCC CGCAAGGCCG 2581 CCCGCCAGGC CCGCGCCGAT CCACCGGTCG CCACCATGGT GAGCAAGGGC GAGGAGGATA 2641 ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC TCCGTGAACG 2701 GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC ACCCAGACCG 2761 CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC CTGTCCCCTC 2821 AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC GACTACTTGA 2881 AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT GAACTTCGAG GACGGCGGCG 2941 TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA GTTCATCTAC AAGGTGAAGC 3001 TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GAAGAAGACC ATGGGCTGGG 3061 AGGCCTCCTC CGAGCGGATG TACCCCGAGG ACGGCGCCCT GAAGGGCGAG ATCAAGCAGA 3121 GGCTGAAGCT GAAGGACGGC GGCCACTACG ACGCTGAGGT CAAGACCACC TACAAGGCCA 3181 AGAAGCCCGT GCAGCTGCCC GGCGCCTACA ACGTCAACAT CAAGTTGGAC ATCACCTCCC 3241 ACAACGAGGA CTACACCATC GTGGAACAGT ACGAACGCGC CGAGGGCCGC CACTCCACCG 3301 GCGGCATGGA CGAGCTGTAC AAGTCCGGAC TCAGATCTCG ACCGGGCACC ATGGCCCCTA 3361 AGAAGAAGAG GAAGGTGGAG GCGTTGCTTC GAATTCTGCA GTCGACGCAA GCTGGGGATC 3421 TCGAGCAGAA GCTGATCAGC GAGGAGGACC TGTAAgtcga caatcaacct ctggattaca 3481 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 3541 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 3601 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 3661 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 3721 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 3781 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 3841 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga 3901 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 3961 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 4021 gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac ctttaagacc 4081 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 4141 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg 4201 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 4261 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 4321 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 4381 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 4441 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 4501 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 4561 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg 4621 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 4681 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 4741 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt 4801 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc 4861 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt 4921 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg 4981 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 5041 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 5101 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 5161 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 5221 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 5281 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 5341 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt 5401 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 5461 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 5521 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 5581 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 5641 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 5701 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 5761 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 5821 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 5881 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 5941 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 6001 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 6061 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 6121 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 6181 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 6241 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 6301 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 6361 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 6421 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 6481 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 6541 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 6601 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 6661 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 6721 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 6781 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 6841 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 6901 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 6961 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 7021 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 7081 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 7141 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 7201 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 7261 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 7321 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 7381 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 7441 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 7501 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta 7561 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc a

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.