pLV3-CMV-CDKN1A-3×HA-EF1a-mChe

pLV3-CMV-CDKN1A-3×HA-EF1a-mCherry-Puro plasmid

PVT92727

pLV3-CMV-CDKN1A-3×HA-EF1a-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-CDKN1A(human)-3×HA-EF1a-mCherry-Puro
Alias: NM_000389.5;CAP20; CDKN1; CIP1; MDA-6; P21; p21CIP1;SDI1; WAF1
Gene lenght: 492bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CDKN1A-3×HA-EF1a-mCherry-Puro plasmid Description

pLV3-CMV-CDKN1A-3×HA-EF1a-mCherry-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-CDKN1A-3×HA-EF1a-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatgtcaga accggctggg gatgtccgtc agaacccatg 2341 cggcagcaag gcctgccgcc gcctcttcgg cccagtggac agcgagcagc tgagccgcga 2401 ctgtgatgcg ctaatggcgg gctgcatcca ggaggcccgt gagcgatgga acttcgactt 2461 tgtcaccgag acaccactgg agggtgactt cgcctgggag cgtgtgcggg gccttggcct 2521 gcccaagctc taccttccca cggggccccg gcgaggccgg gatgagttgg gaggaggcag 2581 gcggcctggc acctcacctg ctctgctgca ggggacagca gaggaagacc atgtggacct 2641 gtcactgtct tgtacccttg tgcctcgctc aggggagcag gctgaagggt ccccaggtgg 2701 acctggagac tctcagggtc gaaaacggcg gcagaccagc atgacagatt tctaccactc 2761 caaacgccgg ctgatcttct ccaagaggaa gcccggatcc TACCCATACG ACGTACCTGA 2821 TTATGCAACT TATCCTTATG ATGTACCTGA TTATGCTAGC TATCCTTATG ATGTCCCAGA 2881 CTACGCATAG gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag tgggcagagc 2941 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct 3001 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc 3061 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca 3121 acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg 3181 cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg 3241 cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac 3301 cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt 3361 gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca 3421 gatccaagct gtgaccggcg cctacgctag acgccaccat ggtgagcaag ggcgaggagg 3481 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga 3541 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga 3601 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc 3661 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact 3721 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg 3781 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga 3841 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct 3901 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc 3961 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg 4021 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct 4081 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca 4141 ccggcggcat ggacgagctg tacaagggat ctcgcgaggg cagaggaagt cttctaacat 4201 gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg gtgcgcctcg 4261 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc 4321 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag 4381 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg 4441 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga 4501 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag 4561 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct 4621 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg 4681 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct 4741 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct 4801 ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa 4861 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 4921 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 4981 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 5041 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 5101 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 5161 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 5221 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 5281 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 5341 tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc 5401 tttggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc 5461 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat 5521 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc 5581 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 5641 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 5701 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat 5761 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg 5821 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 5881 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc 5941 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 6001 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 6061 ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt 6121 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 6181 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 6241 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 6301 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 6361 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 6421 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 6481 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 6541 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 6601 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 6661 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 6721 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 6781 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 6841 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 6901 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 6961 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 7021 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 7081 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 7141 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 7201 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 7261 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 7321 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 7381 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 7441 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 7501 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 7561 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 7621 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 7681 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 7741 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 7801 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 7861 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 7921 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 7981 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 8041 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 8101 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 8161 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 8221 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 8281 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 8341 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 8401 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 8461 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 8521 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 8581 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 8641 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 8701 acgacgttgt aaaacgacgg ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.