pCMV-mTurquoise2-ICA1-Neo plas

pCMV-mTurquoise2-ICA1-Neo plasmid

PVT92721

pCMV-mTurquoise2-ICA1-Neo plasmid Information

Plasmid name: pCMV-mTurquoise2-ICA1(human)-Neo
Alias: NM_022307.3;ICA69; ICAp69
Gene lenght: 1452bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Cyan
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-mTurquoise2-ICA1-Neo plasmid Description

pCMV-mTurquoise2-ICA1-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-mTurquoise2-ICA1-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gCTAGCgcca 601 ccATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG 661 ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT 721 ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA 781 CCCTCGTGAC CACCCTGAGC TGGGGCGTGC AGTGCTTCGC CCGCTACCCC GACCACATGA 841 AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT 901 TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC 961 TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC 1021 ACAAGCTGGA GTACAACTAC TTCAGCGACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA 1081 ACGGCATCAA GGCCAACTTC AAGATCCGCC ACAACATCGA GGACGGCGGC GTGCAGCTCG 1141 CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC 1201 ACTACCTGAG CACCCAGTCC AAGCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG 1261 TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGg 1321 gtggaggcgg atcaggaggc ggtggatcta tgtcaggaca caaatgcagt tatccctggg 1381 acttacagga tcgatatgct caagataagt cagttgtaaa taagatgcaa cagaaatatt 1441 gggagacgaa gcaggccttt attaaagcca cagggaagaa ggaagatgaa catgttgttg 1501 cctctgacgc ggacctggat gccaagctag agctgtttca ttcaattcag agaacctgtc 1561 tggacttatc gaaagcaatt gtactctatc aaaagaggat atgtttcttg tctcaagaag 1621 aaaacgaact gggaaaattt cttcgatccc aaggtttcca agataaaacc agagcaggaa 1681 agatgatgca agcgacagga aaggccctct gcttttcttc ccagcaaagg ttggccttac 1741 gaaatccttt gtgtcgattt caccaagaag tggagacttt tcggcatcgg gccatctcag 1801 atacttggct gacggtgaac cgcatggaac agtgcaggac ggaatataga ggagcactat 1861 tatggatgaa ggacgtgtct caggagcttg atccagacct ctacaagcaa atggagaagt 1921 tcaggaaggt acaaacacaa gtgcgccttg caaaaaaaaa ctttgacaaa ttgaagatgg 1981 atgtttgtca aaaagtggat cttcttggag cgagcagatg caatctcttg tctcacatgc 2041 tagcaacata ccagaccact ctgcttcatt tttgggagaa aacttctcac actatggcag 2101 ccatccatga gagtttcaaa ggttatcaac catatgaatt tactacttta aagagcttac 2161 aagaccctat gaaaaaatta gttgagaaag aagagaagaa gaaaatcaac cagcaggaaa 2221 gtacagatgc agccgtgcag gagccgagcc aattaatttc attagaggaa gaaaaccagc 2281 gcaaggaatc ctctagtttt aagactgaag atggaaaaag tattttatct gccttagaca 2341 aaggctctac acatactgca tgctcaggac ccatagatga actattagac atgaaatctg 2401 aggaaggtgc ttgcctggga ccagtggcag ggaccccgga acctgaaggt gctgacaaag 2461 atgacctgct gctgttgagt gagatcttca atgcttcctc cttggaagag ggcgagttca 2521 gcaaagagtg ggccgctgtg tttggagacg gccaagtgaa ggagccagtg cccactatgg 2581 ccctgggaga gccagacccc aaggcccaga caggctcagg tttccttcct tcgcagcttt 2641 tagaccaaaa tatgaaagac ttacaggcct cgctacaaga acctgctaag gctgcctcag 2701 acctgactgc ctggttcagc ctcttcgctg acctcgaccc actctcaaat cctgatgctg 2761 ttgggaaaac cgataaagaa cacgaattgc tcaatgcatg aGAATTCTGC AGTCGACGGT 2821 ACCGCGGGCC CGGGATCCAC CGGATctaga taactgatca taatcagcca taccacattt 2881 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa 2941 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc 3001 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 3061 tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt 3121 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat 3181 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa 3241 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg 3301 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa 3361 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc 3421 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag 3481 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg 3541 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 3601 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 3661 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg 3721 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 3781 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 3841 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 3901 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 3961 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 4021 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca 4081 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 4141 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 4201 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc 4261 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 4321 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 4381 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 4441 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 4501 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 4561 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct 4621 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 4681 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 4741 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 4801 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 4861 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 4921 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 4981 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg 5041 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca 5101 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg 5161 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc 5221 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc 5281 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat 5341 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 5401 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 5461 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 5521 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 5581 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 5641 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 5701 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 5761 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 5821 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 5881 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 5941 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 6001 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 6061 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 6121 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.