pCMV-EGFP-Ifih1-3×HA-Neo plasm

pCMV-EGFP-Ifih1-3×HA-Neo plasmid

PVT92692

pCMV-EGFP-Ifih1-3×HA-Neo plasmid Information

Plasmid name: pCMV-EGFP-Ifih1(mouse)-3×HA-Neo
Alias: NM_027835.3;9130009C22Rik; Helicard; Hlcd; MDA5; RLR-2
Gene lenght: 3075bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pCMV-EGFP-Ifih1-3×HA-Neo plasmid Description

pCMV-EGFP-Ifih1-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFP-Ifih1-3×HA-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCgcc accatggtga gcaagggcga ggagctgttc 901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 1561 atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagG CGGCGGAGGG 1621 AGTGGAGGAG GGGGAAGCgg aggcggaggg agtAAGCTTa tgtcgattgt ctgttctgca 1681 gaggacagct tcaggaatct catcttattc ttcaggccca ggctgaaaat gtacattcag 1741 gtggagccag tgctggacca cctcatcttt ctgtctgcag aaaccaaaga gcagattctt 1801 aaaaagatca acacctgtgg taacaccagc gcggcagaac tgctgctgag caccttggag 1861 cagggacaat ggcctctggg atggacgcag atgttcgtgg aggccctaga gcacagtggc 1921 aatcccctag ccgcgcgcta tgtcaaaccc acactcactg atctgccctc tccttcctct 1981 gagactgccc atgacgagtg tctccacttg ctgaccctcc tccagcccac tttggtggac 2041 aaacttctga ttaacgatgt cttggacact tgcttcgaga agggactatt gacagtcgaa 2101 gacagaaatc ggatttctgc tgcaggaaac agcgggaatg agtcaggtgt aagagagctg 2161 ctgagaagga ttgtgcagaa ggaaaactgg ttttctacct tcctggatgt tctgcgccaa 2221 actggaaatg atgcactatt ccaagaacta acaggtggag gctgcccaga agacaacaca 2281 gacttggcta actcgtctca cagagatggg cctgcagcta atgagtgtct tctgcctgct 2341 gtcgatgagt caagtctgga gacagaggcc tggaacgtag acgacatatt accagaggct 2401 tcttgtacag attcgtctgt gaccacagaa tcagacacaa gtttggcaga aggaagtgtc 2461 agctgcttcg atgaaagtct tggacataac agcaacatgg gcagggattc aggcaccatg 2521 ggaagtgatt cagatgaaag tgtcatccag acaaaaagag tatcccccga gccagaactg 2581 cagctcaggc cttaccaaat ggaagtggcc caaccagctc tagatgggaa gaatattatt 2641 atctgcctcc ccacggggag tgggaaaacc agagtggctg tttacatcac caaagatcac 2701 ttagacaaga agaagcaggc atctgaatcc gggaaggtta tcgttcttgt caataaggta 2761 atgttagcag aacaactttt ccgaaaagag ttcaacccat atttgaagaa atggtatcga 2821 attattggat taagtggcga tacccagctg aaaatatcat ttccagaagt tgtcaaatct 2881 tacgatgtta ttatcagcac tgctcaaatc cttgaaaact ccctcttaaa tctggagagt 2941 ggagacgatg acggtgtgca gctgtcagac ttctctctca ttatcattga tgagtgccat 3001 cacaccaaca aggaggcagt ctataacaac atcatgagac gatatttgaa gcagaagctg 3061 agaaacaatg acctcaagaa acaaaacaaa ccagccattc ccctgccgca gatactagga 3121 ctgacagctt cacctggtgt tggagcagcc aaaaagcagt ctgaggctga aaaacatatt 3181 ttaaatatat gtgccaatct tgatgccttt accattaaaa cagtgaaaga gaatcttggt 3241 caactcaaac accaaataaa ggaaccatgc aagaaatttg tgattgctga tgacaccaga 3301 gaaaatccat ttaaagagaa acttctagaa attatggcaa gcattcagac ttactgccaa 3361 aaaagtccaa tgtcagattt tggaacccaa cattatgagc agtgggccat tcaaatggag 3421 aaaaaagctg ctaaagacgg aaatcgcaaa gatcgcgtct gtgcagaaca tttgaggaag 3481 tacaacgaag ccctacaaat caacgacacg atccgaatga ttgatgcata tagccacctg 3541 gagacattct acactgatga gaaagaaaag aagttcgcag tcctcaatga cagcgacaag 3601 agtgatgacg aggccagcag ttgcaatgac caacttaagg gcgatgtaaa gaaatctttg 3661 aaactggacg aaacggatga atttctcatg aatttgttct ttgataacaa gaaaatgttg 3721 aaaaaactag ctgaaaaccc aaaatacgag aatgaaaaac tcattaaatt aagaaacacg 3781 atactggaac aattcacaag gtctgaggag tcctcccgag gaattatttt tacaaaaaca 3841 cgacagagca cctacgcact ttcccagtgg atcatggaaa atgcaaagtt tgcggaagtt 3901 ggagtcaaag cgcatcacct gattggcgcg gggcacagca gtgaagtcaa gcccatgact 3961 cagactgaac aaaaagaagt cattagtaaa tttcgcactg gcgaaataaa tctgcttatc 4021 gctacgacgg tggcagagga aggcctggat atcaaagagt gcaatattgt tattcgttat 4081 ggccttgtca cgaacgagat agccatggtc caggcccggg gtcgagccag agctgatgaa 4141 agcacgtatg tcctggtcac cagcagtggc tcaggagtta ccgaacggga gattgttaat 4201 gatttccgag agaagatgat gtataaagct attaaccgtg ttcaaaacat gaaaccagag 4261 gagtatgcac ataagatttt ggaattgcag gtgcaaagta tcctggaaaa gaaaatgaaa 4321 gtcaaaagaa gcattgcaaa gcaatacaac gacaatccat cgttaataac acttctctgc 4381 aaaaattgta gcatgctggt ctgctcggga gaaaacatcc atgtcattga gaagatgcat 4441 catgtcaata tgacaccaga attcaaggga ctctacattg taagagaaaa caaagcactg 4501 caaaagaaat ttgctgatta tcagaccaat ggagagatta tctgcaagtg tggccaggct 4561 tggggaacaa tgatggtgca caaaggttta gatttgcctt gtcttaaaat aaggaatttt 4621 gtagtcaatt tcaaaaataa ctcaccgaag aaacagtaca agaagtgggt ggaattgcct 4681 atcagatttc ctgatcttga ctactcagaa tactgcttgt atagtgatga agatGGTACC 4741 TACCCATACG ACGTACCTGA TTATGCAACT TATCCTTATG ATGTACCTGA TTATGCTAGC 4801 TATCCTTATG ATGTCCCAGA CTACGCATAG GATCCACTAG TCCAGTGTGG TGGAATTCTG 4861 CAGATATCCA GCACAGTGGC GGCCGCTCGA GTCTAGAGGG CCCGTTTAAA CCCGCTGATC 4921 AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC 4981 CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC 5041 GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG 5101 GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA 5161 GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT 5221 AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC 5281 GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA 5341 AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC 5401 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT 5461 TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC 5521 AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC 5581 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT 5641 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 5701 ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 5761 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC 5821 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 5881 TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA 5941 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC 6001 GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC 6061 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA 6121 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT 6181 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG 6241 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA 6301 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 6361 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG 6421 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG 6481 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC 6541 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT 6601 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 6661 TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT 6721 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT 6781 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC 6841 TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA 6901 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA 6961 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG 7021 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT 7081 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC 7141 CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT 7201 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG 7261 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT 7321 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT 7381 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 7441 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 7501 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG 7561 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 7621 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 7681 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 7741 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG 7801 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT 7861 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 7921 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT 7981 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC 8041 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 8101 CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 8161 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 8221 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 8281 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 8341 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 8401 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 8461 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 8521 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 8581 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 8641 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 8701 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 8761 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 8821 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 8881 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 8941 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 9001 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 9061 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 9121 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 9181 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 9241 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 9301 CATTTCCCCG AAAAGTGCCA CCTGACGTCG ACGGATCGGG AGATCTCCCG ATCCCCTATG 9361 GT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.