pLV3-CMV-VPS72-6×His-CopGFP-Pu

pLV3-CMV-VPS72-6×His-CopGFP-Puro plasmid

PVT92592

pLV3-CMV-VPS72-6×His-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-VPS72(human)-6×His-CopGFP-Puro
Alias: NM_001271087.2;CFL1; Swc2; TCFL1; YL-1; YL1;
Gene lenght: 1125bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-VPS72-6×His-CopGFP-Puro plasmid Description

pLV3-CMV-VPS72-6×His-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-VPS72-6×His-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatga gtttggctgg gggccgggca 2341 ccccggaaga ccgctgggaa ccggctttct gggcttttgg aggcagagga ggaagatgag 2401 ttctaccaga cgacttatgg gggtttcaca gaggaatccg gagatgatga gtatcaaggg 2461 gaccagtcag acacagagga cgaagtggac tctgactttg acattgatga aggggatgaa 2521 ccatccagtg atggagaagc agaagagcca agaaggaagc gccgagtagt caccaaggcc 2581 tataaggaac ctctcaagag cttaaggcct cgaaaggtca acaccccggc tggtagctct 2641 cagaaggcgc gagaagagaa ggcactactg ccattagaac tacaagatga cggctctgac 2701 agtcggaagt ctatgcgtca gtctacagct gagcatacac gacaaacgtt ccttcgggta 2761 caggagaggc agggccagtc aagacggcga aaggggcccc actgtgagcg gccactaacc 2821 caggaggaac tgctccggga ggccaagatc acagaagagc ttaatttacg gtcactggag 2881 acatatgagc ggctcgaggc tgataaaaag aagcaggttc ataagaagcg gaagtgcccc 2941 gggcccataa tcacctatca ttcagtgaca gtgccacttg ttggggagcc aggccccaag 3001 gaagagaacg ttgacataga aggctctttg tgtttttcct tgtcttttgt cctcagactt 3061 gatcctgctc cctcggtgtc tgcattgact cctcatgctg ggactggacc cgtcaacccc 3121 cctgctcgct gctcacgtac cttcatcact tttagtgatg atgcaacttt cgaggaatgg 3181 ttcccccaag ggcggccccc aaaagtccct gttcgtgagg tctgtccagt gacccatcgt 3241 ccagccctat accgggaccc tgttacagac ataccctatg ccactgctcg agccttcaag 3301 atcattcgtg aggcttacaa gaagtacatt actgcccatg gactgccgcc cactgcctca 3361 gccctgggcc ccggcccgcc acctcctgag cccctccctg gctctgggcc ccgagccttg 3421 cgccagaaaa ttgtcattaa aCTCGAGCAT CATCACCATC ACCATTAgga tccCTGCGAT 3481 ATCCAGCgcg gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca 3541 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga 3601 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 3661 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 3721 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc 3781 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct 3841 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg 3901 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc 3961 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat 4021 ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag agcggcctgc 4081 ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg 4141 tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca 4201 aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc 4261 acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg 4321 gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca 4381 gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc 4441 ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc 4501 tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg 4561 acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc 4621 ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca 4681 gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc atcgccttcg 4741 ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca 4801 ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg 4861 gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg 4921 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc 4981 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 5041 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 5101 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 5161 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 5221 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 5281 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 5341 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 5401 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg 5461 cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 5521 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 5581 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 5641 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 5701 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 5761 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 5821 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc 5881 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 5941 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 6001 gtctccgcct tcgccctcag acgagtcgga tctccctttg gccgcctccc cgcctggtac 6061 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 6121 ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg 6181 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 6241 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 6301 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 6361 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 6421 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 6481 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 6541 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc 6601 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 6661 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag 6721 agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 6781 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 6841 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 6901 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 6961 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 7021 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 7081 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 7141 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 7201 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 7261 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 7321 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 7381 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 7441 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 7501 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 7561 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 7621 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 7681 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 7741 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 7801 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 7861 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 7921 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 7981 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 8041 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 8101 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 8161 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 8221 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 8281 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 8341 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 8401 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 8461 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 8521 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 8581 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 8641 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 8701 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 8761 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 8821 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 8881 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 8941 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg 9001 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 9061 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 9121 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 9181 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg 9241 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 9301 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 9361 tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.