pCMV-TTLL11-3×Myc-Neo plasmid

pCMV-TTLL11-3×Myc-Neo plasmid

PVT92531

pCMV-TTLL11-3×Myc-Neo plasmid Information

Plasmid name: pCMV-TTLL11(human)-3×Myc-Neo
Alias: NM_001139442.2;bA244O19.1; C9orf148; C9orf20; TTLL11-IT1
Gene lenght: 2130bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-TTLL11-3×Myc-Neo plasmid Description

pCMV-TTLL11-3×Myc-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-TTLL11-3×Myc-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatgcg gcggggcagc 721 tccgagagcg agctggcggc ccggtgggag gcggaggcgg tggctgcggc caaagcggcg 781 gccaaagctg aggccgaggc cacagcggag acggtggcgg aacaggtccg cgtggacgcg 841 ggcgcggccg gggaaccgga gtgcaaggca ggggaggagc agcccaaggt cctggccccg 901 gccccggcgc agcccagtgc ggctgaggag gggaacaccc aggtccttca gcggccgccg 961 cccacgctgc ccccgtccaa gccgaagccg gtgcagggcc tctgcccgca cgggaagccc 1021 cgggacaagg gccgaagctg caagcggagc tcgggccacg gttccggcga gaacggctcc 1081 cagcggccgg tcaccgtgga cagctccaag gccaggacct ccctggatgc cctgaagatc 1141 agcatccgcc agctcaagtg gaaggagttc ccatttggcc ggcgcttgcc ttgtgacatc 1201 tactggcatg gagtttcatt tcacgacaat gacatattct ccggtcaagt gaacaagttt 1261 ccaggcatga cggagatggt gcgtaaaatt actctgagca gagcagtgag aaccatgcag 1321 aatctctttc ctgaggagta caacttctac cctcgctcat ggattctgcc tgacgagttc 1381 cagctctttg ttgctcaggt tcaaatggtg aaagacgatg acccctcctg gaagcccact 1441 tttatcgtga aacctgatgg tggttgtcag ggtgatggaa tctacctcat taaagacccc 1501 agtgacatcc gcctggcagg gaccctccag agcaggccag cggtggtcca ggagtacatc 1561 tgcaaacctc tccttatcga caagctcaag tttgatattc gtctgtatgt cttactcaag 1621 tccttagacc ccttagagat ttatatagcc aaagacggac tctctaggtt ttgtaccgag 1681 ccatatcagg agcccacccc caaaaacctg caccgcatct ttatgcactt aaccaactat 1741 tcactgaaca tccacagcgg caacttcatc cactcggaca gtgctagcac tggcagcaaa 1801 aggacttttt ccagcatcct ttgtagactg tcttccaaag gcgttgacat caagaaggtc 1861 tggtctgaca tcatctccgt ggtgattaag acggtcatcg cgctgactcc agagctcaaa 1921 gtcttctacc agtcagacat ccccacgggg aggccgggcc ccacgtgctt ccagatttta 1981 ggctttgaca ttcttctaat gaaaaatctg aagcctatac tacttgaagt aaatgcaaat 2041 cccagtatga gaatcgaaca tgagcacgaa ctttctccag gggtgtttga aaatgtcccc 2101 agcctcgttg atgaagaagt gaaagtggct gtgatcagag acactctgcg cctcatggac 2161 ccacttaaga agaaaagaga gaatcagtct cagcagcttg aaaaaccatt cgctggaaag 2221 gaagatgctt tggacggcga gctgaccagt gctccagact gcaacgccaa ccccgaagcc 2281 cacctgcctt ccatttgcct caagcaggtg ttccccaagt acgcaaaaca gttcaactac 2341 ctgcgcctgg tggacaggat ggcaaatttg tttatccggt tcctgggcat caaggggaca 2401 atgaagttgg ggccaacagg ctttcgtacc ttcataagga gctgcaaact cagcagcagc 2461 agcctgtcca tggctgccgt ggacatcctc tacattgaca tcacacggag gtggaactcc 2521 atgaccctgg accagcggga ctcagggatg tgtctgcagg ccttcgtaga agctttcttt 2581 ttcctggctc agaggaagtt caagatgctg ccacttcatg agcaggtggc ctcactgatt 2641 gacctttgcg agtaccacct gtccctgctg gatgaaaaac gcctggtgtg tggccggggc 2701 gtcccgtcgg ggggccggcc cccacaccgt ggccctcccc aggagccctc cccctcggcc 2761 cagccagcag gggacaaccc cccaccccgc accagctgtg ccaataagct ctcccatccc 2821 agacataccc tgtccCTCGA GGAACAAAAA CTAATATCAG AGGAAGATTT GGAACAGAAA 2881 CTAATCAGTG AAGAAGATTT AGAGCAAAAG TTAATTTCCG AGGAGGACTT ATAGTGAGGG 2941 CCCGATATCT CGACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC 3001 TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG 3061 CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC 3121 TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG 3181 ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG 3241 CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA 3301 CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAG CTGACGTCCT 3361 TTCCATGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG 3421 TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC 3481 CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC 3541 CGCCTGGAAA CGGGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG AAGGAACCCG 3601 CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGG TGTTGGGTCG TTTGTTCATA 3661 AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA CCCCATTGGG 3721 GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG GGTGAAGGCC 3781 CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AGCAGATCTG CGCAGCTGGG 3841 GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 3901 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 3961 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGCATCC 4021 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG 4081 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 4141 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 4201 TCTATTCTTT TGATTTATAA GGGATTTTGG GGATTTCGGC CTATTGGTTA AAAAATGAGC 4261 TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG 4321 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC 4381 AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT 4441 CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC 4501 CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA 4561 GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG 4621 CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG 4681 ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG 4741 GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC 4801 CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG 4861 TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT 4921 TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG 4981 CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT 5041 CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA 5101 CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA 5161 GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA 5221 GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA 5281 TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC 5341 GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA 5401 ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC 5461 CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGC GAAATGACCG 5521 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 5581 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 5641 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 5701 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 5761 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 5821 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 5881 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 5941 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 6001 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 6061 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 6121 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 6181 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 6241 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 6301 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 6361 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 6421 TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 6481 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 6541 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 6601 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 6661 ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 6721 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT 6781 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 6841 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 6901 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 6961 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 7021 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 7081 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 7141 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 7201 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 7261 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 7321 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 7381 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 7441 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 7501 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 7561 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 7621 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 7681 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 7741 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 7801 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 7861 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 7921 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 7981 TGCCACCTGA CGTCGACGGA TCGGGAGATC TCCCGATCCC CTATGGTCGA CTCTCAGTAC 8041 AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATCTGCTC CCTGCTTGTG TGTTGGAGGT 8101 CGCTGAGTAG TGCGCGAGCA AAATTTAAGC TACAACAAGG CAAGGCTTGA CCGACAATTG 8161 CATGAAGAAT CTGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.