pMD2.G-Ubc-Tax-3×FLAG plasmid

pMD2.G-Ubc-Tax-3×FLAG plasmid

PVT95830

pMD2.G-Ubc-Tax-3×FLAG plasmid Information

Plasmid name: pMD2.G-Ubc-Tax-3×FLAG
Alias:
Gene lenght: 1062bp
Host: Mammalian cells,lentivirus
Use(s): Packaging assistant
Fragment type: ORF
Species: Human T-cell leukemia virusItype
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Ubc
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: enCMV-R
Copy number: High
Induction:

pMD2.G-Ubc-Tax-3×FLAG plasmid Description

pMD2.G-Ubc-Tax-3×FLAG is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pMD2.G-Ubc-Tax-3×FLAG plasmid Sequence

1 tggaggcaag gcctgcaaaa tgcaatactg caagcattgg ggagtcagac tcccatcagg 61 tgtctggttc gagatggctg ataaggatct ctttgctgca gccagattcc ctgaatgccc 121 agaagggtca agtatctctg ctccatctca gacctcagtg gatgtaagtc taattcagga 181 cgttgagagg atcttggatt attccctctg ccaagaaacc tggagcaaaa tcagagcggg 241 tcttccaatc tctccagtgg atctcagcta tcttgctcct aaaaacccag gaaccggtcc 301 tgctttcacc ataatcaatg gtaccctaaa atactttgag accagataca tcagagtcga 361 tattgctgct ccaatcctct caagaatggt cggaatgatc agtggaacta ccacagaaag 421 ggaactgtgg gatgactggg caccatatga agacgtggaa attggaccca atggagttct 481 gaggaccagt tcaggatata agtttccttt atacatgatt ggacatggta tgttggactc 541 cgatcttcat cttagctcaa aggctcaggt gttcgaacat cctcacattc aagacgctgc 601 ttcgcaactt cctgatgatg agagtttatt ttttggtgat actgggctat ccaaaaatcc 661 aatcgagctt gtagaaggtt ggttcagtag ttggaaaagc tctattgcct cttttttctt 721 tatcataggg ttaatcattg gactattctt ggttctccga gttggtatcc atctttgcat 781 taaattaaag cacaccaaga aaagacagat ttatacagac atagagatga accgacttgg 841 aaagtaactc aaatcctgca caacagattc ttcatgtttg gaccaaatca acttgtgata 901 ccatgctcaa agaggcctca attatatttg agtttttaat ttttatgaaa aaaaaaaaaa 961 aaaacggaat tcaccccacc agtgcaggct gcctatcaga aagtggtggc tggtgtggct 1021 aatgccctgg cccacaagta tcactaagct cgctttcttg ctgtccaatt tctattaaag 1081 gttcctttgt tccctaagtc caactactaa actgggggat attatgaagg gccttgagca 1141 tctggattct gcctaataaa aaacatttat tttcattgca atgatgtatt taaattattt 1201 ctgaatattt tactaaaaag ggaatgtggg aggtcagtgc atttaaaaca taaagaaatg 1261 aagagctagt tcaaaccttg ggaaaataca ctatatctta aactccatga aagaaggtga 1321 ggctgcaaac agctaatgca cattggcaac agcccctgat gcctatgcct tattcatccc 1381 tcagaaaagg attcaagtag aggcttgatt tggaggttaa agttttgcta tgctgtattt 1441 tacattactt attgttttag ctgtcctcat gaatgtcttt tcactaccca tttgcttatc 1501 ctgcatctct cagccttgac tccactcagt tctcttgctt agagatacca cctttcccct 1561 gaagtgttcc ttccatgttt tacggcgaga tggtttctcc tcgcctggcc actcagcctt 1621 agttgtctct gttgtcttat agaggtctac ttgaagaagg aaaaacaggg ggcatggttt 1681 gactgtcctg tgagcccttc ttccctgcct cccccactca cagtgacccg gaatccctcg 1741 acatggcagt ctagcactag tgcggccgca gatctgcttc ctcgctcact gactcgctgc 1801 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 1861 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 1921 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 1981 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 2041 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 2101 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 2161 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 2221 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 2281 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 2341 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 2401 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 2461 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 2521 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 2581 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 2641 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 2701 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 2761 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 2821 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 2881 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 2941 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 3001 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 3061 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 3121 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 3181 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 3241 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 3301 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 3361 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 3421 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 3481 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 3541 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 3601 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 3661 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtggat cccctgaggg 3721 ggcccggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag 3781 cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag 3841 gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc agaaggacat 3901 tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga gcggaacagg 3961 cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg gcggtgaacg 4021 ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc agccgggatt 4081 tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta gcgggctgct 4141 gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc gtgtggagag 4201 accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg gggttggggg 4261 gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc 4321 tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca aggtcttgag 4381 gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca ccatctgggg 4441 accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt tgcgggggcg 4501 gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc 4561 gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac ctgccggtag 4621 gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg taggctctcc 4681 tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt cagtttcttt 4741 ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga actatgcgct 4801 cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg taatcatttg 4861 ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt 4921 tggctttttt gttagacgga tcccgtggag actcctcaag cgagctggcc accatgccca 4981 agacccgtcg gaggccccgc cgatcccaaa gaaaaagacc accaacatcc atggcccact 5041 tcccagggtt tggacagagt cttcttttcg gatacccagt ctacgtgttt ggagactgtg 5101 tacaaggcga ctggtgcccc atctctgggg gactatgttc ggcccgccta catcgtcacg 5161 ccctactggc cacctgtcca gagcatcaga tcacctggga ccccatcgat ggacgcgtta 5221 tcggctcagc tctacagttc cttatccctc gactcccctc cttccccacc cagagaacct 5281 ctaagaccct taaggtcctt accccgccaa tcactcatac aacccccaac attccaccct 5341 ccttcctcca ggccatgcgc aaatactccc ccttccgaaa tggatacatg gaacccaccc 5401 ttgggcagca cctcccaacc ctgtcttttc cagaccccgg actccggccc caaaacctgt 5461 acaccctctg gggaggctcc gttgtctgca tgtacctcta ccagctttcc ccccccatca 5521 cctggcccct cctgccccat gtgatttttt gccaccccgg ccagctcggg gccttcctca 5581 ccaatgttcc ctacaaacga atagaaaaac tcctctataa aatttccctt accacagggg 5641 ccctaataat tctacccgag gactgtttgc ccaccaccct tttccagcct gctagggcac 5701 ccgtcacgct gacagcctgg caaaacggcc tccttccgtt ccactcaacc ctcaccactc 5761 caggccttat ttggacattt accgatggca cgcctatgat ttccgggccc tgccctaaag 5821 atggccagcc atctttagta ctacagtcct cctcctttat atttcacaaa tttcaaacca 5881 aggcctacca cccctcattt ctactctcac acggcctcat acagtactct tcctttcata 5941 atttgcatct cctatttgaa gaatacacca acatccccat ttctctactt tttaacgaaa 6001 aagaggcaga tgacaatgac catgagcccc aaatatcccc cgggggctta gagcctctca 6061 gtgaaaaaca tttccgtgaa acagaagtcG ACTACAAAGA CCATGACGGT GATTATAAAG 6121 ATCATGACAT CGACTACAAG GATGACGATG ACAAGTAGtg tacatttata ttggctcatg 6181 tccaacatta ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac 6241 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg 6301 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 6361 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac 6421 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 6481 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 6541 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta 6601 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga 6661 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa 6721 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag 6781 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca 6841 tagaagacac cgggaccgat ccagcctccc ctcgaagctt acatgtggta ccgagctcgg 6901 atcctgagaa cttcagggtg agtctatggg acccttgatg ttttctttcc ccttcttttc 6961 tatggttaag ttcatgtcat aggaagggga gaagtaacag ggtacacata ttgaccaaat 7021 cagggtaatt ttgcatttgt aattttaaaa aatgctttct tcttttaata tacttttttg 7081 tttatcttat ttctaatact ttccctaatc tctttctttc agggcaataa tgatacaatg 7141 tatcatgcct ctttgcacca ttctaaagaa taacagtgat aatttctggg ttaaggcaat 7201 agcaatattt ctgcatataa atatttctgc atataaattg taactgatgt aagaggtttc 7261 atattgctaa tagcagctac aatccagcta ccattctgct tttattttat ggttgggata 7321 aggctggatt attctgagtc caagctaggc ccttttgcta atcatgttca tacctcttat 7381 cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa 7441 agcacgtgag atctgaattc tgacactatg aagtgccttt tgtacttagc ctttttattc 7501 attggggtga attgcaagtt caccatagtt tttccacaca accaaaaagg aaactggaaa 7561 aatgttcctt ctaattacca ttattgcccg tcaagctcag atttaaattg gcataatgac 7621 ttaataggca cagccttaca agtcaaaatg cccaagagtc acaaggctat tcaagcagac 7681 ggttggatgt gtcatgcttc caaatgggtc actacttgtg atttccgctg gtatggaccg 7741 aagtatataa cacattccat ccgatccttc actccatctg tagaacaatg caaggaaagc 7801 attgaacaaa cgaaacaagg aacttggctg aatccaggct tccctcctca aagttgtgga 7861 tatgcaactg tgacggatgc cgaagcagtg attgtccagg tgactcctca ccatgtgctg 7921 gttgatgaat acacaggaga atgggttgat tcacagttca tcaacggaaa atgcagcaat 7981 tacatatgcc ccactgtcca taactctaca acctggcatt ctgactataa ggtcaaaggg 8041 ctatgtgatt ctaacctcat ttccatggac atcaccttct tctcagagga cggagagcta 8101 tcatccctgg gaaaggaggg cacagggttc agaagtaact actttgctta tgaaac

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.