pCMV-EGFP-VWCE-3×FLAG-Puro pla

pCMV-EGFP-VWCE-3×FLAG-Puro plasmid

PVT91664

pCMV-EGFP-VWCE-3×FLAG-Puro plasmid Information

Plasmid name: pCMV-EGFP-VWCE(human)-3×FLAG-Puro
Alias: NM_152718.2;URG11; VWC1
Gene lenght: 2865bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pCMV-EGFP-VWCE-3×FLAG-Puro plasmid Description

pCMV-EGFP-VWCE-3×FLAG-Puro is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFP-VWCE-3×FLAG-Puro plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 AAGCTTTCTA GAGAATTCga tatcGGATCC gccaccatgg tgagcaaggg cgaggagctg 961 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc 1021 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 1081 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc 1141 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 1201 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 1261 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 1321 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc 1381 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 1441 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 1501 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 1561 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 1621 gggatcactc tcggcatgga cgagctgtac aagggcggtg gaggcagtgg tggaggcggt 1681 agcGGTACCa tgtgggccgg actgctcctt cgggccgcct gtgtcgcgct cctgctgccg 1741 ggggcaccag cccgaggcta caccgggagg aagccgcccg ggcacttcgc ggccgagaga 1801 cgccgactgg gcccccacgt ctgcctctct gggtttggga gtggctgctg ccctggctgg 1861 gcgccctcta tgggtggtgg gcactgcacc ctgcccctct gctccttcgg ctgtgggagt 1921 ggcatctgca tcgctcccaa tgtctgctcc tgccaggatg gagagcaagg ggccacctgc 1981 ccagaaaccc atggaccatg tggggagtac ggctgtgacc ttacctgcaa ccatggaggc 2041 tgtcaggagg tggcccgagt gtgccccgtg ggcttctcga tgacggagac agctgttggc 2101 atcaggtgta cagacattga cgaatgtgta acctcctcct gcgagggcca ctgtgtgaac 2161 acagaaggtg ggtttgtgtg cgagtgtggg ccgggcatgc agctgtctgc cgaccgccac 2221 agctgccaag acactgacga atgcctaggg actccctgtc agcagagatg taaaaacagc 2281 attggcagct acaagtgttc ctgtcgaact ggcttccacc ttcatggcaa ccggcactcc 2341 tgtgtagatg taaacgagtg tcggaggcca ttggagaggc gagtctgtca ccattcctgc 2401 cacaacaccg tgggcagctt cctatgcaca tgccgacctg gcttcaggct ccgagctgac 2461 cgcgtgtcct gtgaagcttt cccgaaagcc gtgctggccc catctgccat cctgcaaccc 2521 cggcaacacc cgtccaagat gcttctgttg cttcctgagg ccggccggcc tgccctgtcc 2581 ccaggacata gccctccttc tggggctcca gggcccccag ccggagtcag gaccacccgc 2641 ctgccatctc ccaccccacg actacccaca tcctcccctt ctgcccctgt gtggctgctg 2701 tccaccctgc tggccacccc agtgcctact gcctccctgc tggggaacct cagacccccc 2761 tcactccttc agggggaggt gatggggacc ccttcctcac ccaggggccc tgagtccccc 2821 cgactggcag cagggccctc tccctgctgg cacctgggag ccatgcatga atcaaggagt 2881 cgctggacag agcctgggtg ttcccagtgc tggtgcgagg acgggaaggt gacctgtgaa 2941 aaggtgaggt gtgaagctgc ttgttcccac ccaattccct ccagagatgg tgggtgctgc 3001 ccatcgtgca caggctgttt tcacagtggt gtcgtccgag ctgaagggga tgtgttttca 3061 cctcccaatg agaactgcac cgtctgtgtc tgtctggctg gaaacgtgtc ctgcatctct 3121 cctgagtgtc cttctggccc ctgtcagacc cccccacaga cggattgctg tacttgtgtt 3181 ccagtgagat gctatttcca cggccggtgg tacgcagacg gggctgtgtt cagtgggggt 3241 ggtgacgagt gtaccacctg tgtttgccag aatggggagg tggagtgctc cttcatgccc 3301 tgccctgagc tggcctgccc ccgagaagag tggcggctgg gccctgggca gtgttgcttc 3361 acctgccagg agcccacacc ctcgacaggc tgctctcttg acgacaacgg ggttgagttt 3421 ccgattggac agatctggtc gcctggtgac ccctgtgagt tatgcatctg ccaggcagat 3481 ggctcggtga gctgcaagag gacagactgt gtggactcct gccctcaccc gatccggatc 3541 cctggacagt gctgcccaga ctgttcagca ggctgcacct acacaggcag aatcttctat 3601 aacaacgaga ccttcccgtc tgtgctggac ccatgtctga gctgcatctg cctgctgggc 3661 tcagtggcct gttcccccgt ggactgcccc atcacctgta cctacccttt ccaccctgac 3721 ggggagtgct gccccgtgtg ccgagactgc aactacgagg gaaggaaggt ggcgaatggc 3781 caggtgttca ccttggatga tgaaccctgc acccggtgca cgtgccagct gggagaggtg 3841 agctgtgaga aggttccctg ccagcgggcc tgtgccgacc ctgccctgct tcctggggac 3901 tgctgctctt cctgtccaga ttccctgtct cctctggaag aaaagcaggg gctctcccct 3961 cacggaaatg tggcattcag caaagctggt cggagcctgc atggagacac tgaggcccct 4021 gtcaactgta gctcctgtcc tgggcccccg acagcatcac cctcgaggcc ggtgcttcat 4081 ctcctccagc tccttttaag aacgaacttg atgaaaacac agactttacc tacaagcccg 4141 gcaggagctc atggtccaca ctcactcgct ttggggctga cagccacttt cccaggggag 4201 cctggggcct cccctcgact ctcaccaggg ccttcgaccc ctccaggagc ccccactcta 4261 cctctagctt ccccaggggc tcctcagcca cctcctgtga ctccagagcg ctcgttctca 4321 gcctctgggg cccagatagt gtccaggtgg cctcctctgc ctggcaccct cctgacggaa 4381 gcttcagcac tttccatgat ggaccccagc ccctcgaaga cccccatcac cctcctcggg 4441 cctcgcgtgc tttctcccac cacctctaga ctctccacag cccttgcagc caccacccac 4501 cctggccccc agcagccccc agtgggggct tctcgggggg aagagtccac catgCTCGAG 4561 ACCGGTGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA TTACAAGGAT 4621 GACGATGACA AGTGACCGGT aAGTTTAAAC CGCTGATCAG CCTCGACTGT GCCTTCTAGT 4681 TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT 4741 CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT 4801 TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC 4861 AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC 4921 TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 4981 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 5041 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 5101 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 5161 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 5221 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 5281 TATTCTTTTG ATTTATAAGG GATTTTGGCC ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 5341 ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA 5401 AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA 5461 CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 5521 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA 5581 GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG 5641 CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT 5701 TTTGCAAAAA GCTCCCggga tgaccgagta caagcccacg gtgcgcctcg ccacccgcga 5761 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg 5821 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct 5881 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc 5941 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg 6001 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc 6061 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca 6121 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc 6181 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct 6241 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac 6301 ccgcaagccc ggtgcctgaT TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG 6361 AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA 6421 CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA 6481 CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA 6541 TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA 6601 TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT 6661 TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA 6721 GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT 6781 GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC 6841 GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG 6901 CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 6961 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 7021 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 7081 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 7141 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 7201 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 7261 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 7321 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 7381 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 7441 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 7501 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 7561 CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 7621 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 7681 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 7741 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC 7801 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC 7861 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC 7921 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC 7981 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC 8041 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT 8101 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC 8161 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA 8221 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT 8281 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG 8341 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC 8401 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA 8461 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT 8521 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT 8581 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG 8641 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA 8701 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT 8761 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTC

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.