pLV3-CMV-SLC7A11-3×FLAG-Hyg pl

pLV3-CMV-SLC7A11-3×FLAG-Hyg plasmid

PVT91642

pLV3-CMV-SLC7A11-3×FLAG-Hyg plasmid Information

Plasmid name: pLV3-CMV-SLC7A11(human)-3×FLAG-Hyg
Alias: NM_014331.4;CCBR1; xCT
Gene lenght: 1503bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SLC7A11-3×FLAG-Hyg plasmid Description

pLV3-CMV-SLC7A11-3×FLAG-Hyg It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SLC7A11-3×FLAG-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcc GGATCCGCCA CCatggtcag aaagcctgtt gtgtccacca tctccaaagg 2341 aggttacctg cagggaaatg ttaacgggag gctgccttcc ctgggcaaca aggagccacc 2401 tgggcaggag aaagtgcagc tgaagaggaa agtcacttta ctgaggggag tctccattat 2461 cattggcacc atcattggag caggaatctt catctctcct aagggcgtgc tccagaacac 2521 gggcagcgtg ggcatgtctc tgaccatctg gacggtgtgt ggggtcctgt cactatttgg 2581 agctttgtct tatgctgaat tgggaacaac tataaagaaa tctggaggtc attacacata 2641 tattttggaa gtctttggtc cattaccagc ttttgtacga gtctgggtgg aactcctcat 2701 aatacgccct gcagctactg ctgtgatatc cctggcattt ggacgctaca ttctggaacc 2761 attttttatt caatgtgaaa tccctgaact tgcgatcaag ctcattacag ctgtgggcat 2821 aactgtagtg atggtcctaa atagcatgag tgtcagctgg agcgcccgga tccagatttt 2881 cttaaccttt tgcaagctca cagcaattct gataattata gtccctggag ttatgcagct 2941 aattaaaggt caaacgcaga actttaaaga cgccttttca ggaagagatt caagtattac 3001 gcggttgcca ctggcttttt attatggaat gtatgcatat gctggctggt tttacctcaa 3061 ctttgttact gaagaagtag aaaaccctga aaaaaccatt ccccttgcaa tatgtatatc 3121 catggccatt gtcaccattg gctatgtgct gacaaatgtg gcctacttta cgaccattaa 3181 tgctgaggag ctgctgcttt caaatgcagt ggcagtgacc ttttctgagc ggctactggg 3241 aaatttctca ttagcagttc cgatctttgt tgccctctcc tgctttggct ccatgaacgg 3301 tggtgtgttt gctgtctcca ggttattcta tgttgcgtct cgagagggtc accttccaga 3361 aatcctctcc atgattcatg tccgcaagca cactcctcta ccagctgtta ttgttttgca 3421 ccctttgaca atgataatgc tcttctctgg agacctcgac agtcttttga atttcctcag 3481 ttttgccagg tggcttttta ttgggctggc agttgctggg ctgatttatc ttcgatacaa 3541 atgcccagat atgcatcgtc ctttcaaggt gccactgttc atcccagctt tgttttcctt 3601 cacatgcctc ttcatggttg ccctttccct ctattcggac ccatttagta cagggattgg 3661 cttcgtcatc actctgactg gagtccctgc gtattatctc tttattatat gggacaagaa 3721 acccaggtgg tttagaataa tgtcggagaa aataaccaga acattacaaa taatactgga 3781 agttgtacca gaagaagata agttaggtgg aggcggatca CTCGAGGACT ACAAAGACCA 3841 TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGTAGcggcc 3901 gcaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc 3961 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg 4021 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa 4081 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga 4141 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga 4201 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga 4261 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg 4321 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct 4381 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg 4441 gcgcctacgt cgacatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga 4501 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg 4561 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg 4621 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg 4681 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac 4741 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg 4801 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat 4861 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg 4921 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc 4981 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc 5041 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg 5101 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga 5161 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc 5221 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc 5281 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa 5341 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg 5401 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc 5461 gtccgagggc aaaggaatag gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat 5521 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 5581 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 5641 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 5701 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 5761 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 5821 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 5881 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 5941 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 6001 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 6061 gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 6121 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa 6181 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 6241 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 6301 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 6361 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 6421 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt 6481 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 6541 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc 6601 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 6661 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 6721 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc 6781 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 6841 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 6901 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 6961 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 7021 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 7081 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 7141 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 7201 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 7261 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 7321 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 7381 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 7441 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 7501 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 7561 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 7621 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 7681 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 7741 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 7801 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 7861 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 7921 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 7981 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 8041 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 8101 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 8161 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 8221 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 8281 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 8341 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 8401 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 8461 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 8521 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 8581 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 8641 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 8701 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 8761 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 8821 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 8881 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 8941 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg 9001 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 9061 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 9121 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt 9181 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca 9241 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg 9301 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac 9361 gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.