pCMV-JAG1-6×His-JAG1-Neo plasm

pCMV-JAG1-6×His-JAG1-Neo plasmid

PVT91626

pCMV-JAG1-6×His-JAG1-Neo plasmid Information

Plasmid name: pCMV-JAG1-6×His-JAG1(human)-Neo
Alias: NM_000214.3;AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;HJ1; JAGL1
Gene lenght: 3558bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-JAG1-6×His-JAG1-Neo plasmid Description

pCMV-JAG1-6×His-JAG1-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-JAG1-6×His-JAG1-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CGCCACCatg cgttccccac ggacgcgcgg ccggtccggg cgccccctaa gcctcctgct 961 cgccctgctc tgtgccctgc gagccaaggt gtgtggggcc tcgggtCATC ACCATCATCA 1021 CCATcagttc gagttggaga tcctgtccat gcagaacgtg aacggggagc tgcagaacgg 1081 gaactgctgc ggcggcgccc ggaacccggg agaccgcaag tgcacccgcg acgagtgtga 1141 cacatacttc aaagtgtgcc tcaaggagta tcagtcccgc gtcacggccg gggggccctg 1201 cagcttcggc tcagggtcca cgcctgtcat cgggggcaac accttcaacc tcaaggccag 1261 ccgcggcaac gaccgcaacc gcatcgtgct gcctttcagt ttcgcctggc cgaggtccta 1321 tacgttgctt gtggaggcgt gggattccag taatgacacc gttcaacctg acagtattat 1381 tgaaaaggct tctcactcgg gcatgatcaa ccccagccgg cagtggcaga cgctgaagca 1441 gaacacgggc gttgcccact ttgagtatca gatccgcgtg acctgtgatg actactacta 1501 tggctttggc tgcaataagt tctgccgccc cagagatgac ttctttggac actatgcctg 1561 tgaccagaat ggcaacaaaa cttgcatgga aggctggatg ggccccgaat gtaacagagc 1621 tatttgccga caaggctgca gtcctaagca tgggtcttgc aaactcccag gtgactgcag 1681 gtgccagtac ggctggcaag gcctgtactg tgataagtgc atcccacacc cgggatgcgt 1741 ccacggcatc tgtaatgagc cctggcagtg cctctgtgag accaactggg gcggccagct 1801 ctgtgacaaa gatctcaatt actgtgggac tcatcagccg tgtctcaacg ggggaacttg 1861 tagcaacaca ggccctgaca aatatcagtg ttcctgccct gaggggtatt caggacccaa 1921 ctgtgaaatt gctgagcacg cctgcctctc tgatccctgt cacaacagag gcagctgtaa 1981 ggagacctcc ctgggctttg agtgtgagtg ttccccaggc tggaccggcc ccacatgctc 2041 tacaaacatt gatgactgtt ctcctaataa ctgttcccac gggggcacct gccaggacct 2101 ggttaacgga tttaagtgtg tgtgcccccc acagtggact gggaaaacgt gccagttaga 2161 tgcaaatgaa tgtgaggcca aaccttgtgt aaacgccaaa tcctgtaaga atctcattgc 2221 cagctactac tgcgactgtc ttcccggctg gatgggtcag aattgtgaca taaatattaa 2281 tgactgcctt ggccagtgtc agaatgacgc ctcctgtcgg gatttggtta atggttatcg 2341 ctgtatctgt ccacctggct atgcaggcga tcactgtgag agagacatcg atgaatgtgc 2401 cagcaacccc tgtttgaatg ggggtcactg tcagaatgaa atcaacagat tccagtgtct 2461 gtgtcccact ggtttctctg gaaacctctg tcagctggac atcgattatt gtgagcctaa 2521 tccctgccag aacggtgccc agtgctacaa ccgtgccagt gactatttct gcaagtgccc 2581 cgaggactat gagggcaaga actgctcaca cctgaaagac cactgccgca cgaccccctg 2641 tgaagtgatt gacagctgca cagtggccat ggcttccaac gacacacctg aaggggtgcg 2701 gtatatttcc tccaacgtct gtggtcctca cgggaagtgc aagagtcagt cgggaggcaa 2761 attcacctgt gactgtaaca aaggcttcac gggaacatac tgccatgaaa atattaatga 2821 ctgtgagagc aacccttgta gaaacggtgg cacttgcatc gatggtgtca actcctacaa 2881 gtgcatctgt agtgacggct gggagggggc ctactgtgaa accaatatta atgactgcag 2941 ccagaacccc tgccacaatg ggggcacgtg tcgcgacctg gtcaatgact tctactgtga 3001 ctgtaaaaat gggtggaaag gaaagacctg ccactcacgt gacagtcagt gtgatgaggc 3061 cacgtgcaac aacggtggca cctgctatga tgagggggat gcttttaagt gcatgtgtcc 3121 tggcggctgg gaaggaacaa cctgtaacat agcccgaaac agtagctgcc tgcccaaccc 3181 ctgccataat gggggcacat gtgtggtcaa cggcgagtcc tttacgtgcg tctgcaagga 3241 aggctgggag gggcccatct gtgctcagaa taccaatgac tgcagccctc atccctgtta 3301 caacagcggc acctgtgtgg atggagacaa ctggtaccgg tgcgaatgtg ccccgggttt 3361 tgctgggccc gactgcagaa taaacatcaa tgaatgccag tcttcacctt gtgcctttgg 3421 agcgacctgt gtggatgaga tcaatggcta ccggtgtgtc tgccctccag ggcacagtgg 3481 tgccaagtgc caggaagttt cagggagacc ttgcatcacc atggggagtg tgataccaga 3541 tggggccaaa tgggatgatg actgtaatac ctgccagtgc ctgaatggac ggatcgcctg 3601 ctcaaaggtc tggtgtggcc ctcgaccttg cctgctccac aaagggcaca gcgagtgccc 3661 cagcgggcag agctgcatcc ccatcctgga cgaccagtgc ttcgtccacc cctgcactgg 3721 tgtgggcgag tgtcggtctt ccagtctcca gccggtgaag acaaagtgca cctctgactc 3781 ctattaccag gataactgtg cgaacatcac atttaccttt aacaaggaga tgatgtcacc 3841 aggtcttact acggagcaca tttgcagtga attgaggaat ttgaatattt tgaagaatgt 3901 ttccgctgaa tattcaatct acatcgcttg cgagccttcc ccttcagcga acaatgaaat 3961 acatgtggcc atttctgctg aagatatacg ggatgatggg aacccgatca aggaaatcac 4021 tgacaaaata atcgatcttg ttagtaaacg tgatggaaac agctcgctga ttgctgccgt 4081 tgcagaagta agagttcaga ggcggcctct gaagaacaga acagatttcc ttgttccctt 4141 gctgagctct gtcttaactg tggcttggat ctgttgcttg gtgacggcct tctactggtg 4201 cctgcggaag cggcggaagc cgggcagcca cacacactca gcctctgagg acaacaccac 4261 caacaacgtg cgggagcagc tgaaccagat caaaaacccc attgagaaac atggggccaa 4321 cacggtcccc atcaaggatt atgagaacaa gaactccaaa atgtctaaaa taaggacaca 4381 caattctgaa gtagaagagg acgacatgga caaacaccag cagaaagccc ggtttgccaa 4441 gcagccggcg tacacgctgg tagacagaga agagaagccc cccaacggca cgccgacaaa 4501 acacccaaac tggacaaaca aacaggacaa cagagacttg gaaagtgccc agagcttaaa 4561 ccgaatggag tacatcgtat agCTCGAGTC TAGAGGGCCC GTTTAAACCC GCTGATCAGC 4621 CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT 4681 GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA 4741 TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA 4801 GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC 4861 GGAAAGAACC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG 4921 CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC 4981 CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC 5041 TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA 5101 AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG 5161 CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC 5221 ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA 5281 TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG 5341 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 5401 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 5461 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 5521 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 5581 ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 5641 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 5701 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 5761 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 5821 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 5881 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 5941 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 6001 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 6061 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 6121 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 6181 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 6241 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 6301 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 6361 GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 6421 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 6481 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 6541 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 6601 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 6661 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 6721 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 6781 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 6841 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 6901 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 6961 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 7021 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 7081 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 7141 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 7201 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 7261 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 7321 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 7381 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 7441 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 7501 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 7561 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 7621 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 7681 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 7741 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 7801 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 7861 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 7921 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 7981 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 8041 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 8101 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 8161 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 8221 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 8281 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 8341 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 8401 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 8461 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 8521 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 8581 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 8641 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 8701 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 8761 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 8821 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 8881 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 8941 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 9001 TTCCCCGAAA AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.