pLV3-CMV-TJP1-3×HA-EF1a-Fluc-P

pLV3-CMV-TJP1-3×HA-EF1a-Fluc-Puro plasmid

PVT91247

pLV3-CMV-TJP1-3×HA-EF1a-Fluc-Puro plasmid Information

Plasmid name: pLV3-CMV-TJP1(human)-3×HA-EF1a-Fluc-Puro
Alias: NM_003257.5;ZO-1
Gene lenght: 5247bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TJP1-3×HA-EF1a-Fluc-Puro plasmid Description

pLV3-CMV-TJP1-3×HA-EF1a-Fluc-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-TJP1-3×HA-EF1a-Fluc-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcGCCAC Catgtccgcc agagctgcgg ccgccaagag cacagcaatg 2341 gaggaaacag ctatatggga acaacataca gtgacgcttc acagggctcc tggatttgga 2401 tttggaattg caatatctgg tggacgagat aatcctcatt ttcagagtgg ggaaacgtca 2461 atagtgattt cagatgtgct gaaaggagga ccagctgaag gacagctaca ggaaaatgac 2521 cgagttgcaa tggttaacgg agtttcaatg gataatgttg aacatgcttt tgctgttcag 2581 caactaagga aaagtgggaa aaatgcaaaa attacaatta gaaggaagaa gaaagttcaa 2641 ataccagtaa gtcgtcctga tcctgaacca gtatctgata atgaagaaga tagttatgat 2701 gaggaaatac atgatccaag aagtggccgg agtggtgtgg ttaacagaag gagtgagaag 2761 atttggccga gggatagaag tgcaagtaga gagaggagct tgtccccgcg gtcagacagg 2821 cggtcagtgg cttccagcca gcctgctaaa cctactaaag tcacactggt gaaatcccgg 2881 aaaaatgaag aatatggtct tcgattggca agccatatat ttgttaagga aatttcacaa 2941 gatagtttgg cagcaagaga tggcaatatt caagaaggtg atgttgtatt gaagataaat 3001 ggtactgtga cagaaaatat gtcattgaca gatgcaaaga cattgataga aaggtctaaa 3061 ggcaaattaa aaatggtagt tcaaagagat gaacgggcta cgctattgaa tgtccctgat 3121 ctttctgaca gcatccactc tgctaatgcc tctgagagag acgacatttc agaaattcag 3181 tcactggcat cagatcattc tggtcgatca cacgataggc ctccccgccg cagccggtca 3241 cgatctcctg accagcggtc agagccttct gatcattcca ggcactcgcc gcagcagcca 3301 agcaatggca gtctccggag tagagatgaa gagagaattt ctaaacctgg ggctgtctca 3361 actcctgtaa agcatgctga tgatcacaca cctaaaacag tggaagaagt tacagttgaa 3421 agaaatgaga aacaaacacc ttctcttcca gaaccaaagc ctgtgtatgc ccaagttggg 3481 caaccagatg tggatttacc tgtcagtcca tctgatggtg tcctacctaa ttcaactcat 3541 gaagatggga ttcttcggcc cagcatgaaa ttggtaaaat tcagaaaagg agatagtgtg 3601 ggtttgcggc tggctggtgg aaatgatgtt ggaatatttg tagctggcgt tctagaagat 3661 agccctgcag ccaaggaagg cttagaggaa ggtgatcaaa ttctcagggt aaacaacgta 3721 gattttacaa atatcataag agaagaagcc gtccttttcc tgcttgacct ccctaaagga 3781 gaagaagtga ccatattggc tcagaagaag aaggatgttt atcgtcgcat tgtagaatca 3841 gatgtaggag attctttcta tattagaacc cattttgaat atgaaaagga atctccctat 3901 ggacttagtt ttaacaaagg agaggtgttc cgtgttgtgg ataccttgta caatggaaaa 3961 ctgggctctt ggcttgctat tcgaattggt aaaaatcata aggaggtaga acgaggcatc 4021 atccctaata agaacagagc tgagcagcta gccagtgtac agtatacact tccaaaaaca 4081 gcaggcggag accgtgctga cttctggaga ttcagaggtc ttcgcagctc caagagaaat 4141 cttcgaaaaa gcagagagga tttgtccgct cagcctgttc aaacaaagtt tccagcttat 4201 gaaagagtgg ttcttcgaga agctggattt ctgaggcctg taaccatttt tggaccaata 4261 gctgatgttg ccagagaaaa gctggcaaga gaagaaccag atatttatca aattgcaaag 4321 agtgaaccac gagacgctgg aactgaccaa cgtagctctg gcattattcg cctgcataca 4381 ataaagcaaa tcatagatca agacaaacat gctttattag atgtaacacc aaatgcagtt 4441 gatcgtctta actatgccca gtggtatcca attgttgtat ttcttaaccc tgattctaag 4501 caaggagtaa aaacaatgag aatgaggtta tgtccagaat ctcggaaaag tgccaggaag 4561 ttatacgagc gatctcataa acttcgtaaa aataatcacc atctttttac aactacaatt 4621 aacttaaatt caatgaatga tggttggtat ggtgcgctga aagaagcaat tcaacaacag 4681 caaaaccagc tggtatgggt ttccgaggga aaggcggatg gtgctacaag tgatgacctt 4741 gatttgcatg atgatcgtct gtcctacctg tcagctccag gtagtgaata ctcaatgtat 4801 agcacggaca gtagacacac ttctgactat gaagacacag acacagaagg cggggcctac 4861 actgatcaag aactagatga aactcttaat gatgaggttg ggactccacc ggagtctgcc 4921 attacacggt cctctgagcc tgtaagagag gactcctctg gaatgcatca tgaaaaccaa 4981 acatatcctc cttactcacc acaagcgcag ccacaaccaa ttcatagaat agactcccct 5041 ggatttaagc cagcctctca acagaaagca gaagcttcat ctccagtccc ttacctttcg 5101 cctgaaacaa acccagcatc atcaacctct gctgttaatc ataatgtaaa tttaactaat 5161 gtcagactgg aggagcccac cccagctcct tccacctctt actcaccaca agctgattct 5221 ttaagaacac caagtactga ggcagctcac ataatgctaa gagatcaaga accatcattg 5281 tcgtcgcatg tagatccaac aaaggtgtat agaaaggatc catatcccga ggaaatgatg 5341 aggcagaacc atgttttgaa acagccagcc gttagtcacc cagggcacag gccagacaaa 5401 gagcctaatc tgacctatga accccaactc ccatacgtag agaaacaagc cagcagagac 5461 ctcgagcagc ccacatacag atacgagtcc tcaagctata cggaccagtt ttctcgaaac 5521 tatgaacatc gtctgcgata cgaagatcgc gtccccatgt atgaagaaca gtggtcatat 5581 tatgatgaca aacagcccta cccatctcgg ccaccttttg ataatcagca ctctcaagac 5641 cttgactcca gacagcatcc cgaagagtcc tcagaacgag ggtactttcc acgttttgaa 5701 gagccagccc ctctgtctta cgacagcaga ccacgttacg aacaggcacc tagagcatcc 5761 gccctgcggc acgaagagca gccagctcct gggtatgaca cacatggtag actcagaccg 5821 gaagcccagc cccacccttc agcagggccc aagcctgcag agtccaagca gtattttgag 5881 caatattcac gcagttacga gcaagtacca ccccaaggat ttacctctag agcaggtcat 5941 tttgagcctc tccatggtgc tgcagctgtc cctccgctga taccttcatc tcagcataag 6001 ccagaagctc tgccttcaaa caccaaacca ctgcctccac ccccaactca aaccgaagaa 6061 gaggaagatc cagcaatgaa gccacagtct gtactcacca gagttaagat gtttgaaaac 6121 aaaagatctg catccttaga gaccaagaag gatgtaaatg acactggcag ttttaagcct 6181 ccagaagtag catctaaacc ttcaggtgct cccatcattg gtcccaaacc cacttctcag 6241 aatcaattca gtgaacatga caaaactctg tacaggatcc cagaacctca aaaacctcaa 6301 ctgaagccac ctgaagatat tgttcggtcc aatcattatg accctgaaga agatgaagaa 6361 tattatcgaa aacagctgtc atactttgac cgaagaagtt ttgagaataa gcctcctgca 6421 cacattgccg ccagccatct ctccgagcct gcaaagccag cgcattctca gaatcaatca 6481 aatttttcta gttattcttc aaagggaaag cctcctgaag ctgatggtgt ggatagatca 6541 tttggcgaga aacgctatga acccatccag gccactcccc ctcctcctcc attgccctcg 6601 cagtatgccc agccatctca gcctgtcacc agcgcgtctc tccacataca ttctaaggga 6661 gcacatggtg aaggtaattc agtgtcattg gattttcaga attccttagt gtccaaacca 6721 gacccacctc catctcagaa taagccagca actttcagac caccaaaccg agaagatact 6781 gctcaggcag ctttctatcc ccagaaaagt tttccagata aagccccagt taatggaact 6841 gaacagactc agaaaacagt cactccagca tacaatcgat tcacaccaaa accatataca 6901 agttctgccc gaccatttga acgcaagttt gaaagtccta aattcaatca caatcttctg 6961 ccaagtgaaa ctgcacataa acctgacttg tcttcaaaaa ctcccacttc tccaaaaact 7021 cttgtgaaat cgcacagttt ggcacagcct cctgagtttg acagtggagt tgaaactttc 7081 tctatccatg cagagaagcc taaatatcaa ataaataata tcagcacagt gcctaaagct 7141 attcctgtga gtccttcagc tgtggaagag gatgaagatg aagatggtca tactgtggtg 7201 gccacagccc gaggcatatt taacagcaat gggggcgtgc tgagttccat agaaactggt 7261 gttagtataa ttatccctca aggagccatt cccgaaggag ttgagcagga aatctatttc 7321 aaggtctgcc gggacaacag catccttcca cctttagata aagagaaagg tgaaacactg 7381 ctgagtcctt tggtgatgtg tggtccccat ggcctcaagt tcctgaagcc tgtggagctg 7441 cgcttaccac actgtgatcc taaaacctgg caaaacaagt gtcttcccgg agatccaaat 7501 tatctcgttg gagcaaactg tgtttctgtc cttattgacc actttctcga gTACCCATAC 7561 GACGTACCTG ATTATGCAAC TTATCCTTAT GATGTACCTG ATTATGCTAG CTATCCTTAT 7621 GATGTCCCAG ACTACGCAta ggatccgcgg ccgcaaggat ctgcgatcgc tccggtgccc 7681 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca 7741 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact 7801 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga 7861 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca 7921 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg 7981 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa 8041 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc 8101 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg 8161 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggaga 8221 gcgacgagag cggcctgatg gaagacgcca aaaacataaa gaaaggcccg gcgccattct 8281 atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag agatacgccc 8341 tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc acttacgctg 8401 agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg ctgaatacaa 8461 atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg 8521 cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc 8581 tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag gggttgcaaa 8641 aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc atggattcta 8701 aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat ctacctcccg 8761 gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca attgcactga 8821 tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct catagaactg 8881 cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc attccggata 8941 ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact acactcggat 9001 atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag ctgtttctga 9061 ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta ttctccttct 9121 tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa attgcttctg 9181 gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc catctgccag 9241 gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt acacccgagg 9301 gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg 9361 atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt gtgagaggtc 9421 ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg attgacaagg 9481 atggatggct acattctgga gacatagctt actgggacga agacgaacac ttcttcatcg 9541 ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc gctgaattgg 9601 aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt cttcccgacg 9661 atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag acgatgacgg 9721 aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag 9781 gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa 9841 tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgaga tcccgcgctc 9901 agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga tctcgcgagg 9961 gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct atgaccgagt 10021 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg 10081 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg 10141 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg 10201 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag 10261 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg 10321 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt 10381 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg 10441 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg 10501 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg 10561 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga aatcaacctc 10621 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 10681 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 10741 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 10801 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 10861 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 10921 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 10981 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg 11041 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 11101 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc cgccttcgcc 11161 ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta agaccaatga 11221 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc 11281 taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct ctctggttag 11341 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 11401 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 11461 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc 11521 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact 11581 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 11641 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 11701 atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 11761 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 11821 ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg gcccaaattc 11881 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 11941 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 12001 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 12061 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 12121 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 12181 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 12241 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 12301 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 12361 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 12421 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 12481 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 12541 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 12601 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 12661 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 12721 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 12781 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 12841 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 12901 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 12961 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 13021 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 13081 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 13141 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 13201 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 13261 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 13321 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 13381 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 13441 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 13501 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 13561 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 13621 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 13681 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 13741 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 13801 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 13861 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 13921 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 13981 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 14041 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 14101 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 14161 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 14221 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 14281 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 14341 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 14401 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 14461 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.