pLV3-CMV-Fluc-PTPRM-3×HA-EF1a-

pLV3-CMV-Fluc-PTPRM-3×HA-EF1a-Puro plasmid

PVT94693

pLV3-CMV-Fluc-PTPRM-3×HA-EF1a-Puro plasmid Information

Plasmid name: pLV3-CMV-Fluc-PTPRM(human)-3×HA-EF1a-Puro
Alias: NM_001105244.2;hR-PTPu; PTPRL1; R-PTP-MU; RPTPM; RPTPU
Gene lenght: 4395bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-Fluc-PTPRM-3×HA-EF1a-Puro plasmid Description

pLV3-CMV-Fluc-PTPRM-3×HA-EF1a-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Fluc-PTPRM-3×HA-EF1a-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTGC 2281 CACCatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctagagga 2341 tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac 2401 aattgctttt acagatgcac atatcgaggt gaacatcacg tacgcggaat acttcgaaat 2461 gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt 2521 cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg 2581 agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatgaa 2641 catttcgcag cctaccgtag tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt 2701 gcaaaaaaaa ttaccaataa tccagaaaat tattatcatg gattctaaaa cggattacca 2761 gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata 2821 cgattttgta ccagagtcct ttgatcgtga caaaacaatt gcactgataa tgaactcctc 2881 tggatctact gggttaccta agggtgtggc ccttccgcat agaactgcct gcgtcagatt 2941 ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag 3001 tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg 3061 atttcgagtc gtcttaatgt atagatttga agaagagctg tttttacgat cccttcagga 3121 ttacaaaatt caaagtgcgt tgctagtacc aaccctattt tcattcttcg ccaaaagcac 3181 tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctgggg gcgcacctct 3241 ttcgaaagaa gtcggggaag cggttgcaaa acgcttccat cttccaggga tacgacaagg 3301 atatgggctc actgagacta catcagctat tctgattaca cccgaggggg atgataaacc 3361 gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg 3421 gaaaacgctg ggcgttaatc agagaggcga attatgtgtc agaggaccta tgattatgtc 3481 cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca 3541 ttctggagac atagcttact gggacgaaga cgaacacttc ttcatagttg accgcttgaa 3601 gtctttaatt aaatacaaag gataccaggt ggcccccgct gaattggagt cgatattgtt 3661 acaacacccc aacatcttcg acgcgggcgt ggcaggtctt cccgacgatg acgccggtga 3721 acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt 3781 ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt 3841 ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct 3901 cataaaggcc aagaagggcg gaaagtccaa attgggtgga ggcggatcag gaggcggtgg 3961 atctGAATTC atgaggggac ttgggacttg cctggcgact ttggccggac ttttgctaac 4021 tgcggcgggc gagacgttct caggtggctg cctctttgat gagccgtata gcacatgtgg 4081 atatagtcaa tctgaaggtg atgacttcaa ttgggagcaa gtgaacacct tgactaaacc 4141 gacttctgat ccatggatgc catcaggttc tttcatgctg gtgaatgcct ctgggagacc 4201 tgaggggcag agagcccacc tgctcttacc ccaacttaaa gaaaatgaca cccactgcat 4261 cgattttcac tattttgtgt ccagcaagag taattctcct ccggggttac tcaatgtcta 4321 cgtgaaggtc aataacgggc cactggggaa tcctatctgg aatatatctg gagacccaac 4381 acgtacatgg aacagggcag aactggccat tagtactttc tggcctaact tttatcaggt 4441 gatttttgaa gtgataactt ctggacatca aggctatctc gctatcgatg aggtgaaggt 4501 gttaggacat ccatgtacca ggactcctca cttcctgcgg attcagaatg tggaagttaa 4561 tgctggccag tttgctacct tccagtgcag tgccatcggc aggaccgtgg caggagacag 4621 gctctggtta cagggcattg atgtgcgaga tgctcctctg aaggaaatca aggtgaccag 4681 ctcccgacgc ttcattgctt catttaatgt tgtgaatacc accaaacgag atgctggaaa 4741 gtaccgctgc atgattcgca ctgaaggagg tgttggaata tcaaactatg cagagttggt 4801 agttaaagaa ccacccgttc ctattgcccc acctcagctc gcctctgtag gagccaccta 4861 cctgtggata cagctcaacg ccaactccat caatggggat gggcccattg tggcccgaga 4921 ggtggagtac tgcacggcca gtgggagctg gaatgaccgg cagccagtcg attccacgag 4981 ctataaaatt ggacaccttg acccagatac agaatatgag attagtgtgc tcctgaccag 5041 gccaggggag ggtggcactg gctctcctgg tccagctctc aggacaagaa caaagtgtgc 5101 tgatcccatg cgaggcccaa gaaaactaga agtagtggag gtcaaatctc ggcaaatcac 5161 tatccgctgg gagccatttg gatataatgt aactcgttgc cacagttata atctcactgt 5221 ccactactgt taccaagttg gaggacaaga acaagtgcga gaagaagtaa gctgggatac 5281 agaaaactca caccctcaac acacgatcac taacctgtca ccatacacca atgtcagtgt 5341 gaaactgatc ctcatgaacc cagagggccg gaaggaaagc caagaactca tagtgcagac 5401 agatgaagac ctcccaggtg ctgttcccac tgaatccata caaggaagta cctttgaaga 5461 gaagatattt cttcagtgga gagaaccaac tcaaacatat ggtgtaatca ctttatatga 5521 gatcacctac aaagcagtca gttcctttga cccagaaata gatttatcca atcagagtgg 5581 aagagtttca aagctgggaa atgaaaccca ttttctgttt tttggactgt atccggggac 5641 cacatactcc tttaccatcc gagctagcac agctaagggt tttgggcctc cagcaacaaa 5701 ccagttcacc accaaaatat cagcaccctc tatgccagct tatgaacttg agacaccttt 5761 gaatcaaact gacaataccg tgacagtcat gctgaaacct gcccacagca gaggagcacc 5821 tgtcagtgtc tatcaaatag ttgttgagga agaacgtcct cgaagaacta aaaagacgac 5881 agaaatctta aagtgctacc cagtgccaat tcacttccag aatgcttctc tgctgaactc 5941 acagtactac tttgctgcag aatttcctgc agacagcctc caagctgcgc agccttttac 6001 aattggtgat aataagacat ataatggata ctggaacact ccccttctcc cctataaaag 6061 ctacagaatt tatttccaag ctgctagtag agccaatggg gaaaccaaaa tagactgtgt 6121 ccaagtggcc acaaaaggag ctgccactcc gaaaccagtc ccagaacccg agaaacagac 6181 agaccataca gttaaaattg ctggagtcat cgcgggcatc ttgctgttcg tgattatatt 6241 tcttggagtt gtgttggtaa tgaagaaaag gaaactggcc aagaagcgga aagagaccat 6301 gagcagcacc cgacaggaga tgactgtgat ggtgaactca atggacaaga gctatgctga 6361 gcagggcaca aactgcgacg aggctttctc attcatggac acgcacaatc tgaatgggag 6421 atctgtgtct tcaccatcgt ccttcacaat gaaaacaaat acactgagca catcggtgcc 6481 taattcctat tacccagacc catttgtgcc aactgcaatt ttagtgccaa taaatgatga 6541 aacccacaca atggccagcg ataccagcag cctggtgcag tcccatactt acaagaagcg 6601 agagccggcc gacgtgccct atcagactgg gcagctccac cccgccatcc gggtggcaga 6661 cctccttcag cacatcacac agatgaagtg tgcggagggc tacggcttca aggaggaata 6721 cgagagcttc tttgaagggc agtctgcacc atgggactcg gctaagaaag atgagaacag 6781 aatgaagaac agatacggga atatcattgc atacgatcat tcccgagtga ggctgcagac 6841 aatagaagga gacacaaact cagactatat caatggcaat tatatcgatg gttatcatcg 6901 acccaatcat tacattgcta cccaagggcc aatgcaggaa accatctatg acttctggag 6961 gatggtgtgg cacgaaaaca ctgcaagtat catcatggtg accaatcttg tggaagtggg 7021 aagggtcaaa tgctgcaaat actggccaga tgacacagag atatataaag acattaaagt 7081 taccctaata gaaacagaac tactggcaga atatgtgata agaacatttg ctgttgaaaa 7141 gagaggtgtg catgaaatcc gagagatcag acagtttcac ttcactggct ggccggatca 7201 tggggtcccc taccatgcca ccggcctgct gggattcgtg cggcaagtca agtccaagag 7261 cccgcccagt gcaggcccac tggtggtgca ctgcagtgct ggtgcaggga ggactggctg 7321 tttcatcgtc attgatatca tgttggacat ggccgaaagg gaaggggtcg tagacatcta 7381 caactgcgtc agggagctgc ggtcacggag ggtgaacatg gtgcaaacag aggagcagta 7441 tgtgtttatc cacgatgcga tcctggaagc ctgtctttgt ggggacacct ctgtgcctgc 7501 ttcccaagtt aggtctctgt attatgacat gaacaaactg gatccacaga caaactcaag 7561 ccagattaaa gaggaattcc ggacgctaaa catggtgaca ccaacgctgc gagtagagga 7621 ctgcagcatc gcactgttgc cccggaacca tgagaaaaac cggtgcatgg acatcctgcc 7681 cccagaccgc tgcctgccct tcctcatcac catcgatggg gagagcagca actacatcaa 7741 tgctgccctc atggacagct ataaacagcc ttcagctttt atagtcaccc agcatccttt 7801 gccaaacaca gtgaaagact tttggagact ggtcctggat tatcactgca catccgtagt 7861 tatgctaaat gatgtggatc ctgcccagtt gtgtccacag tactggccag aaaacggagt 7921 acacagacac ggccccatcc aggtggaatt tgtctctgct gacctggaag aggacatcat 7981 cagcaggata ttccgcattt acaatgccgc cagaccccaa gatggatatc ggatggtgca 8041 gcaattccag ttcctgggct ggccgatgta cagggacaca ccagtgtcta agcgctcctt 8101 cttgaagctc attcgccagg tggacaagtg gcaagaggag tacaatggcg gggaaggccg 8161 cacggttgtg cactgcttga acgggggagg ccgcagtggg acgttctgcg ccatcagcat 8221 cgtatgtgag atgctccggc accagagaac cgtggatgtc tttcacgctg tgaagacact 8281 gaggaacaac aagcccaaca tggtcgacct cctggatcag tacaagttct gctacgaggt 8341 ggccctggaa tacttgaatt ctggcTACCC ATACGACGTA CCTGATTATG CAACTTATCC 8401 TTATGATGTA CCTGATTATG CTAGCTATCC TTATGATGTC CCAGACTACG CATAGGGATC 8461 CGCGGCCGCG AAGGATCTGC GATCGCTCCG GTGCCCGTCA GTGGGCAGAG CGCACATCGC 8521 CCACAGTCCC CGAGAAGTTG GGGGGAGGGG TCGGCAATTG AACGGGTGCC TAGAGAAGGT 8581 GGCGCGGGGT AAACTGGGAA AGTGATGTCG TGTACTGGCT CCGCCTTTTT CCCGAGGGTG 8641 GGGGAGAACC GTATATAAGT GCAGTAGTCG CCGTGAACGT TCTTTTTCGC AACGGGTTTG 8701 CCGCCAGAAC ACAGCTGAAG CTTCGAGGGG CTCGCATCTC TCCTTCACGC GCCCGCCGCC 8761 CTACCTGAGG CCGCCATCCA CGCCGGTTGA GTCGCGTTCT GCCGCCTCCC GCCTGTGGTG 8821 CCTCCTGAAC TGCGTCCGCC GTCTAGGTAA GTTTAAAGCT CAGGTCGAGA CCGGGCCTTT 8881 GTCCGGCGCT CCCTTGGAGC CTACCTAGAC TCAGCCGGCT CTCCACGCTT TGCCTGACCC 8941 TGCTTGCTCA ACTCTACGTC TTTGTTTCGT TTTCTGTTCT GCGCCGTTAC AGATCCAAGC 9001 TGTGACCGGC GCCTACGCTA GATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC 9061 GACGACGTCC CCCGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG 9121 CGCCACACCG TCGACCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC 9181 CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG 9241 GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG 9301 CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG 9361 GCGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC 9421 CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC 9481 GCCGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG 9541 CTCGGCTTCA CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG 9601 ACCCGCAAGC CCGGTGCCTG AGTCGACAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA 9661 TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG 9721 CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC 9781 TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC 9841 ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT 9901 TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT 9961 GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG 10021 AAATCATCGT CCTTTCCTTG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG 10081 TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG 10141 CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT 10201 TGGGCCGCCT CCCCGCCTGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT 10261 TAGCCACTTT TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAAATA 10321 AGATCTGCTT TTTGCTTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT 10381 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA 10441 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA 10501 GTCAGTGTGG AAAATCTCTA GCAGTAGTAG TTCATGTCAT CTTATTATTC AGTATTTATA 10561 ACTTGCAAAG AAATGAATAT CAGAGAGTGA GAGGAACTTG TTTATTGCAG CTTATAATGG 10621 TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC 10681 TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGCTCT AGCTATCCCG 10741 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 10801 TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 10861 TTTGGAGGCC TAGACTTTTG CAGAGACGGC CCAAATTCGT AATCATGGTC ATAGCTGTTT 10921 CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG 10981 TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG 11041 CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG 11101 GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC 11161 TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC 11221 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG 11281 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT 11341 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG 11401 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA 11461 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG 11521 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT 11581 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC 11641 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC 11701 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT 11761 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC 11821 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC 11881 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG 11941 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG 12001 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG 12061 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT 12121 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA 12181 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA 12241 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC 12301 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT 12361 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG 12421 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC 12481 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG 12541 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA 12601 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA 12661 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA 12721 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG 12781 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT 12841 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA 12901 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT 12961 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 13021 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT 13081 ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC 13141 GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG 13201 TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT 13261 CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG 13321 TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCCA TTCGCCATTC 13381 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG 13441 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA 13501 CGACGTTGTA AAACGACGGC CAGTGCCAAG CTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.