pLV3-CMV-MAP3K1-3×HA-Puro plas

pLV3-CMV-MAP3K1-3×HA-Puro plasmid

PVT91235

pLV3-CMV-MAP3K1-3×HA-Puro plasmid Information

Plasmid name: pLV3-CMV-MAP3K1(human)-3×HA-Puro
Alias: NM_005921.2;MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6
Gene lenght: 4539bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MAP3K1-3×HA-Puro plasmid Description

pLV3-CMV-MAP3K1-3×HA-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-MAP3K1-3×HA-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC CATGGCTGCT GCCGCAGGCA ACCGAGCATC TTCATCAGGA 2341 TTCCCAGGAG CTAGGGCGAC GTCTCCCGAG GCTGGGGGAG GAGGTGGTGC ACTCAAGGCA 2401 TCTAGCGCCC CTGCAGCAGC CGCTGGACTT CTCAGAGAGG CGGGGAGTGG CGGGCGCGAG 2461 AGAGCAGATT GGAGGAGGAG ACAGCTCAGA AAGGTTCGGA GCGTTGAGCT TGATCAACTG 2521 CCTGAGCAGC CTCTTTTCCT GGCAGCTTCC CCACCAGCCT CTTCAACATC CCCGTCCCCC 2581 GAGCCTGCTG ACGCGGCCGG TAGTGGGACA GGCTTTCAGC CAGTAGCCGT GCCCCCCCCT 2641 CATGGTGCTG CCTCCAGGGG AGGAGCCCAC CTGACCGAGA GCGTGGCTGC GCCGGATAGC 2701 GGCGCTTCAA GCCCGGCTGC TGCGGAGCCT GGCGAAAAGC GCGCTCCCGC TGCTGAACCT 2761 AGCCCAGCAG CCGCTCCCGC TGGGAGGGAG ATGGAGAACA AAGAAACTTT GAAGGGGCTC 2821 CATAAAATGG ATGATCGCCC GGAGGAAAGG ATGATACGCG AAAAGCTCAA AGCTACTTGC 2881 ATGCCTGCCT GGAAGCACGA GTGGCTCGAA AGACGAAATC GCAGAGGACC TGTAGTGGTG 2941 AAGCCTATCC CTGTGAAGGG TGATGGGTCC GAGATGAACC ATCTCGCCGC CGAGAGCCCC 3001 GGCGAGGTGC AGGCTAGTGC AGCCTCTCCT GCTAGCAAAG GTAGAAGATC TCCTAGCCCT 3061 GGCAACTCAC CTTCCGGGCG CACGGTGAAG TCAGAAAGCC CAGGTGTCCG CAGAAAGCGG 3121 GTCTCCCCTG TGCCCTTCCA AAGTGGGCGC ATCACTCCTC CTCGAAGAGC CCCAAGTCCT 3181 GATGGGTTCT CTCCGTACTC TCCAGAAGAG ACCAACCGGA GGGTGAACAA AGTGATGAGG 3241 GCTCGCCTGT ACCTCCTCCA GCAGATAGGA CCTAACTCAT TTCTGATCGG TGGGGACTCA 3301 CCAGATAACA AATATCGCGT CTTTATAGGT CCCCAGAATT GTTCCTGCGC TAGGGGCACA 3361 TTCTGCATAC ACCTGCTTTT TGTAATGTTG CGGGTGTTCC AGCTCGAGCC TAGTGATCCA 3421 ATGCTGTGGA GGAAGACTCT CAAGAACTTC GAGGTGGAGA GTTTGTTCCA GAAGTATCAC 3481 TCTCGCCGGA GCTCCAGGAT AAAAGCACCT TCTCGGAACA CCATTCAAAA GTTCGTGTCC 3541 AGGATGAGCA ACAGTCATAC TCTGTCTAGC AGCTCTACTA GTACGAGCAG CTCCGAAAAC 3601 AGCATTAAGG ACGAGGAGGA GCAAATGTGT CCAATCTGTC TGCTGGGAAT GCTGGACGAG 3661 GAAAGTTTGA CAGTGTGCGA GGATGGCTGC CGAAACAAAC TGcaccacca ctgcatgtca 3721 atttgggcag aagagtgtag aagaaataga gaacctttaa tatgtcccct ttgtagatct 3781 aagtggagat ctcatgattt ctacagccac gagttgtcaa gtcctgtgga ttccccttct 3841 tccctcagag ctgcacagca gcaaaccgta cagcagcagc ctttggctgg atcacgaagg 3901 aatcaagaga gcaattttaa ccttactcat tatggaactc agcaaatccc tcctgcttac 3961 aaagatttag ctgagccatg gattcaggtg tttggaatgg aactcgttgg ctgcttattt 4021 tctagaaact ggaatgtgag agagatggcc ctcaggcgtc tttcccatga tgtcagtggg 4081 gccctgctgt tggcaaatgg ggagagcact ggaaattctg ggggcagcag tggaagcagc 4141 ccgagtgggg gagccaccag tgggtcttcc cagaccagta tctcaggaga tgtggtggag 4201 gcatgctgca gcgttctgtc aatggtctgt gctgaccctg tctacaaagt gtacgttgct 4261 gctttaaaaa cattgagagc catgctggta tatactcctt gccacagttt agcggaaaga 4321 atcaaacttc agagacttct ccagccagtt gtagacacca tcctagtcaa atgtgcagat 4381 gccaatagcc gcacaagtca gctgtccata tcaacactgt tggaactgtg caaaggccaa 4441 gcaggagagt tggcagttgg cagagaaata ctaaaagctg gatccattgg tattggtggt 4501 gttgattatg tcttaaattg tattcttgga aaccaaactg aatcaaacaa ttggcaagaa 4561 cttcttggcc gcctttgtct tatagataga ctgttgttgg aatttcctgc tgaattttat 4621 cctcatattg tcagtactga tgtttcacaa gctgagcctg ttgaaatcag gtataagaag 4681 ctgctgtccc tcttaacctt tgctttgcag tccattgata attcccactc aatggttggc 4741 aaactttcca gaaggatcta cttgagttct gcaagaatgg ttactacagt accccatgtg 4801 ttttcaaaac tgttagaaat gctgagtgtt tccagttcca ctcacttcac caggatgcgt 4861 cgccgtttga tggctattgc agatgaggtg gaaattgccg aagccatcca gttgggcgta 4921 gaagacactt tggatggtca acaggacagc ttcttgcagg catctgttcc caacaactat 4981 ctggaaacca cagagaacag ttcccctgag tgcacagtcc atttagagaa aactggaaaa 5041 ggattatgtg ctacaaaatt gagtgccagt tcagaggaca tttctgagag actggccagc 5101 atttcagtag gaccttctag ttcaacaaca acaacaacaa caacaacaga gcaaccaaag 5161 ccaatggttc aaacaaaagg cagaccccac agtcagtgtt tgaactcctc tcctttatct 5221 catcattccc aattaatgtt tccagccttg tcaacccctt cttcttctac cccatctgta 5281 ccagctggca ctgcaacaga tgtctctaag catagacttc agggattcat tccctgcaga 5341 ataccttctg catctcctca aacacagcgc aagttttctc tacaattcca cagaaactgt 5401 cctgaaaaca aagactcaga taaactttcc ccagtcttta ctcagtcaag acccttgccc 5461 tccagtaaca tacacaggcc aaagccatct agacctaccc caggtaatac aagtaaacag 5521 ggagatccct caaaaaatag catgacactt gatctgaaca gtagttccaa atgtgatgac 5581 agctttggct gtagcagcaa tagtagtaat gctgttatac ccagtgacga gacagtgttc 5641 accccagtag aggagaaatg cagattagat gtcaatacag agctcaactc cagtattgag 5701 gaccttcttg aagcatctat gccttcaagt gatacaacag taacttttaa gtcagaagtt 5761 gctgtcctgt ctcctgaaaa ggctgaaaat gatgatacct acaaagatga tgtgaatcat 5821 aatcaaaagt gcaaagagaa gatggaagct gaagaagaag aagctttagc aattgccatg 5881 gcaatgtcag cgtctcagga tgccctcccc atagttcctc agctgcaggt tgaaaatgga 5941 gaagatatca tcattattca acaggataca ccagagactc taccaggaca taccaaagca 6001 aaacaaccgt atagagaaga cactgaatgg ctgaaaggtc aacagatagg ccttggagca 6061 ttttcttctt gttatcaggc tcaagatgtg ggaactggaa ctttaatggc tgttaaacag 6121 gtgacttatg tcagaaacac atcttctgag caagaagaag tagtagaagc actaagagaa 6181 gagataagaa tgatgagcca tctgaatcat ccaaacatca ttaggatgtt gggagccacg 6241 tgtgagaaga gcaattacaa tctcttcatt gaatggatgg cagggggatc ggtggctcat 6301 ttgctgagta aatatggagc cttcaaagaa tcagtagtta ttaactacac tgaacagtta 6361 ctccgtggcc tttcgtatct ccatgaaaac caaatcattc acagagatgt caaaggtgcc 6421 aatttgctaa ttgacagcac tggtcagaga ctaagaattg cagattttgg agctgcagcc 6481 aggttggcat caaaaggaac tggtgcagga gagtttcagg gacaattact ggggacaatt 6541 gcatttatgg cacctgaggt actaagaggt caacagtatg gaaggagctg tgatgtatgg 6601 agtgttggct gtgctattat agaaatggct tgtgcaaaac caccatggaa tgcagaaaaa 6661 cactccaatc atcttgcttt gatatttaag attgctagtg caactactgc tccatcgatc 6721 ccttcacatt tgtctcctgg tttacgagat gtggctcttc gttgtttaga acttcaacct 6781 caggacagac ctccatcaag agagctactg aagcatccag tctttcgtac tacatggGGA 6841 TCCTACCCAT ACGACGTACC TGATTATGCA ACTTATCCTT ATGATGTACC TGATTATGCT 6901 AGCTATCCTT ATGATGTCCC AGACTACGCA TAGGCGGCCG CGAAGGATCT GCGATCGCTC 6961 CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT TGGGGGGAGG 7021 GGTCGGCAAT TGAACGGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG AAAGTGATGT 7081 CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA GTGCAGTAGT 7141 CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGCTGA AGCTTCGAGG 7201 GGCTCGCATC TCTCCTTCAC GCGCCCGCCG CCCTACCTGA GGCCGCCATC CACGCCGGTT 7261 GAGTCGCGTT CTGCCGCCTC CCGCCTGTGG TGCCTCCTGA ACTGCGTCCG CCGTCTAGGT 7321 AAGTTTAAAG CTCAGGTCGA GACCGGGCCT TTGTCCGGCG CTCCCTTGGA GCCTACCTAG 7381 ACTCAGCCGG CTCTCCACGC TTTGCCTGAC CCTGCTTGCT CAACTCTACG TCTTTGTTTC 7441 GTTTTCTGTT CTGCGCCGTT ACAGATCCAA GCTGTGACCG GCGCCTACGC TAGATGACCG 7501 AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCCGGGCC GTACGCACCC 7561 TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGACCCG GACCGCCACA 7621 TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA 7681 AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG 7741 AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC 7801 TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT 7861 GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG 7921 TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT 7981 CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG 8041 AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGAGTCGACA 8101 ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC 8161 CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA 8221 TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT 8281 GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG 8341 GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA 8401 TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT 8461 TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG 8521 CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA 8581 ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC 8641 GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCCT GGTACCTTTA 8701 AGACCAATGA CTTACAAGGC AGCTGTAGAT CTTAGCCACT TTTTAAAAGA AAAGGGGGGA 8761 CTGGAAGGGC TAATTCACTC CCAACGAAAA TAAGATCTGC TTTTTGCTTG TACTGGGTCT 8821 CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT 8881 AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC 8941 TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTAGT 9001 AGTTCATGTC ATCTTATTAT TCAGTATTTA TAACTTGCAA AGAAATGAAT ATCAGAGAGT 9061 GAGAGGAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT 9121 TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT 9181 GTATCTTATC ATGTCTGGCT CTAGCTATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT 9241 CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT 9301 CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGACTTT TGCAGAGACG 9361 GCCCAAATTC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA 9421 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 9481 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT 9541 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT 9601 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT 9661 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 9721 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 9781 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 9841 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 9901 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 9961 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 10021 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 10081 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 10141 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 10201 CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 10261 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG 10321 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 10381 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 10441 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 10501 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 10561 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 10621 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 10681 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 10741 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 10801 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG 10861 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT 10921 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 10981 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 11041 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA 11101 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC 11161 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT 11221 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 11281 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 11341 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA 11401 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 11461 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 11521 AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC 11581 GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA 11641 TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC 11701 GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG 11761 AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA 11821 GAAAATACCG CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT 11881 CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT 11941 TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGCCA 12001 AGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.