pCMV-3×HA-Prkaa1-EGFP-Neo plas

pCMV-3×HA-Prkaa1-EGFP-Neo plasmid

PVT91219

pCMV-3×HA-Prkaa1-EGFP-Neo plasmid Information

Plasmid name: pCMV-3×HA-Prkaa1(mouse)-EGFP-Neo
Alias: NM_001013367.3;AMPKalpha1; C130083N04Rik
Gene lenght: 1677bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×HA-Prkaa1-EGFP-Neo plasmid Description

pCMV-3×HA-Prkaa1-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×HA-Prkaa1-EGFP-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaagcc actgggatcc GCCACCatgT ACCCATACGA CGTACCTGAT TATGCAACTT 961 ATCCTTATGA TGTACCTGAT TATGCTAGCT ATCCTTATGA TGTCCCAGAC TACGCAatgc 1021 gcagactcag ttcctggaga aagatggcga cggccgagaa gcagaagcac gacgggcggg 1081 tgaagatcgg ccactacatc ctgggggaca cgcttggtgt cggcaccttc gggaaagtga 1141 aggtgggcaa gcacgagttg accggacata aagtggctgt gaagatactc aaccggcaga 1201 agattcggag ccttgacgtg gtgggaaaaa tccgccggga gattcagaac ctgaagctgt 1261 tcaggcaccc tcacatcatc aaactgtacc aggtcatcag tacaccatct gatattttca 1321 tggtgatgga atatgtctct ggaggagagc tatttgatta tatctgtaaa aatggaaggt 1381 tggacgaaaa ggaaagccgc cgtctgttcc agcagatcct ttccggtgtg gattattgtc 1441 acaggcatat ggtggtccac agagatttga aacctgagaa cgtcctgctt gatgcacaca 1501 tgaatgcaaa gatagccgac tttggtcttt caaacatgat gtcagatggt gaatttttaa 1561 gaacaagctg tggctcaccc aattatgccg caccagaagt catttcagga agattgtacg 1621 caggccccga ggtggacatc tggagcagcg gggtcattct ctatgctttg ctgtgtggaa 1681 ccctcccttt tgatgatgac catgtgccaa ctctttttaa gaagatatgt gatgggatct 1741 tttatacccc tcagtactta aacccttcag taatcagcct tttgaaacat atgctgcagg 1801 tggatcccat gaagagggcc gcaataaaag atatcaggga acacgagtgg tttaaacagg 1861 accttccgaa gtatctcttt cctgaggacc catcttatag ttcaaccatg atcgatgacg 1921 aagccttgaa agaagtgtgt gagaagttcg agtgttcgga ggaggaggtc ctcagctgcc 1981 tgtacaacag aaaccaccag gacccactag ccgtcgccta ccacctcatc atagacaaca 2041 ggagaataat gaatgaagcc aaagatttct acctagcaac cagcccacct gactctttcc 2101 tggacgacca ccatttaact cggcctcacc ctgaaagagt accgttcttg gttgccgaaa 2161 caccacgggc ccggcacacc ctggatgaat taaacccaca gaaatccaaa caccaaggtg 2221 tacggaaggc aaaatggcat ttgggaattc gaagtcaaag ccgacccaat gatatcatgg 2281 cagaagtttg tagagcaatc aagcagttgg attatgaatg gaaggttgta aacccctatt 2341 atttgcgtgt acgaaggaag aatcctgtga caagcacatt ttccaaaatg agtctacagc 2401 tataccaagt ggatagtagg acttacttgt tggatttccg tagtattgat gatgagatta 2461 cagaagccaa atcagggact gctactccac agagatcggg atccatcagc aactatcgat 2521 cttgccaaag gagtgactct gatgccgaag ctcaaggaaa gccctcagac gtctccctta 2581 cctcatctgt cacctccctc gactcctccc ctgtcgacgt agctccaaga ccaggaagtc 2641 atacaataga attttttgaa atgtgtgcaa atctaattaa aattcttgca cagGCctcga 2701 gtggcggtgg aggaagcggc ggaggtggat ctatggtgag caagggcgag gagctgttca 2761 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg 2821 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 2881 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc 2941 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 3001 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 3061 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 3121 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 3181 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc 3241 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 3301 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 3361 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 3421 tcactctcgg catggacgag ctgtacaagt agtctagagg gcccgtttaa acccgctgat 3481 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 3541 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 3601 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 3661 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg 3721 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat 3781 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 3841 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 3901 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc 3961 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 4021 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 4081 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg 4141 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa 4201 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 4261 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 4321 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 4381 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 4441 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg 4501 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt 4561 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca 4621 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 4681 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 4741 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc 4801 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 4861 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 4921 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 4981 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 5041 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 5101 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca 5161 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 5221 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 5281 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 5341 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact 5401 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc 5461 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg 5521 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca 5581 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 5641 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata 5701 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 5761 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 5821 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 5881 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 5941 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 6001 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 6061 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 6121 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 6181 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 6241 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 6301 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 6361 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 6421 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 6481 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 6541 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct 6601 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 6661 accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 6721 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 6781 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 6841 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 6901 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 6961 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 7021 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 7081 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 7141 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 7201 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 7261 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 7321 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 7381 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 7441 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 7501 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 7561 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 7621 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 7681 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 7741 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 7801 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 7861 acatttcccc gaaaagtgcc acctgacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.