pLV2-CMV-EGFP-CPSF3-3×FLAG-Pur

pLV2-CMV-EGFP-CPSF3-3×FLAG-Puro plasmid

PVT96259

pLV2-CMV-EGFP-CPSF3-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-CMV-EGFP-CPSF3(human)-3×FLAG-Puro
Alias: NM_016207.4;CPSF-73; CPSF73; NEDMHS; NEDMHSN
Gene lenght: 2052bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-EGFP-CPSF3-3×FLAG-Puro plasmid Description

pLV2-CMV-EGFP-CPSF3-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-EGFP-CPSF3-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt 2881 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 2941 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 3001 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 3061 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 3121 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 3181 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 3241 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 3301 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 3361 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 3421 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 3481 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 3541 catggacgag ctgtacaagt ccggactcag atctcgagGC GGCGGAGGGA GTGGAGGAGG 3601 GGGAAGCgga ggcggaggga gtggatccat gtctgcgatt cctgctgagg agagcgacca 3661 gctgctgatc cgaccccttg gagctgggca agaagtagga agatcatgta ttattctcga 3721 gttcaaagga agaaaaataa tgctcgactg tgggatccac cctggcctag aaggaatgga 3781 tgctcttcct tatattgatt taattgaccc agctgagatt gatctcctat taattagtca 3841 tttccatttg gatcactgtg gagctctgcc ctggtttcta cagaagacaa gtttcaaagg 3901 aagaacattt atgactcatg ccacaaaagc tatttataga tggcttcttt ctgattatgt 3961 caaagttagt aacatatcag cagacgacat gctgtatacc gagacagatt tggaagaaag 4021 catggacaaa attgaaacta tcaactttca tgaagttaag gaagttgcgg gaatcaagtt 4081 ttggtgttac catgcaggtc acgtcctagg agccgccatg ttcatgattg agatcgcagg 4141 cgtgaagctt ttgtacactg gtgatttctc aagacaagaa gataggcact taatggcagc 4201 tgaaattcct aatattaagc ctgatattct tatcattgaa tctacttatg ggacccatat 4261 ccatgagaaa cgtgaagagc gagaagcaag attctgtaac actgtccacg atattgtaaa 4321 cagaggaggc aggggtctca ttcctgtctt tgctcttgga agggctcagg agctgctctt 4381 gattctagat gagtactggc agaatcaccc agaactacat gacattccaa tatactatgc 4441 atcatctttg gccaagaagt gtatggcagt gtaccagaca tatgtaaatg ccatgaatga 4501 caaaatccgc aaacagatca acatcaataa tccctttgtt ttcaaacaca ttagtaacct 4561 caagagcatg gatcattttg atgacattgg tcccagtgtt gtaatggcct ccccaggcat 4621 gatgcaaagt ggcttatcca gagaattatt tgaaagctgg tgtactgata agaggaatgg 4681 tgtcattata gcgggatact gtgtagaagg gacacttgcc aagcacatca tgtctgaacc 4741 tgaagaaatc actactatgt ctggacagaa gttaccactg aaaatgtctg ttgattacat 4801 ttctttctca gctcacacgg attaccagca aaccagtgaa tttattcgtg ctttgaaacc 4861 gcctcatgtg attttagtcc atggagaaca gaatgaaatg gccagattga aagcagcact 4921 gattcgagaa tatgaagata acgatgaagt tcacatagag gttcataatc ctcggaatac 4981 agaagcagtg accttaaact tcagaggaga aaaactagcc aaggttatgg gatttttagc 5041 agacaaaaaa ccagaacaag gccagcgggt ctcaggaata cttgttaaaa gaaactttaa 5101 ttatcacata ctttctcctt gcgacctgtc caattatact gacctggcca tgagcacggt 5161 gaagcagacc caagccattc catatactgg tccctttaat ttgctctgtt accagctgca 5221 gaaattgaca ggtgatgtgg aagaattaga aattcaagaa aaacctgctc tgaaagtgtt 5281 caaaaatatt actgtaatac aagaaccagg catggtggta ttagaatggc tggcaaaccc 5341 ttctaatgat atgtatgcag atacagtaac aactgtgata ttggaagttc agtcaaatcc 5401 caaaataaga aaaggtgcag tacagaaggt ttctaaaaaa ttagaaatgc acgtttacag 5461 caagaggttg gagatcatgc tccaggacat atttggagaa gactgtgtaa gtgtaaagga 5521 tgactctatt cttagcgtca cagtggacgg gaaaactgcc aaccttaact tggagacacg 5581 gactgtagaa tgtgaagagg gaagtgaaga cgatgaatcc ctccgagaaa tggtggagct 5641 ggctgcacag agactgtacg aggccctgac gccagttcac GGATCCGACT ACAAAGACCA 5701 TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGTAGtctag 5761 ataattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc 5821 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc 5881 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc 5941 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat 6001 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg 6061 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag 6121 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc 6181 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct 6241 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag 6301 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 6361 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 6421 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 6481 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 6541 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 6601 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 6661 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 6721 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 6781 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 6841 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg 6901 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 6961 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 7021 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 7081 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 7141 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 7201 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 7261 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 7321 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 7381 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 7441 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat 7501 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct 7561 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc 7621 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 7681 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt 7741 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg 7801 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca 7861 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg 7921 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca 7981 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct 8041 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg 8101 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct 8161 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 8221 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 8281 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 8341 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 8401 gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg gcgtaatcat 8461 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 8521 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 8581 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 8641 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 8701 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 8761 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 8821 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 8881 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 8941 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 9001 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 9061 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 9121 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 9181 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 9241 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 9301 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 9361 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 9421 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 9481 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 9541 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 9601 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 9661 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 9721 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 9781 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 9841 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 9901 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 9961 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 10021 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 10081 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 10141 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 10201 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 10261 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 10321 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 10381 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 10441 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 10501 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 10561 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg 10621 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc 10681 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 10741 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat 10801 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc 10861 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt 10921 actacaaact tagtagtttt taaagaaatt gtatttgtta aatatgtact acaaacttag 10981 tagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.