pLV3-CMV-CSF2-3×FLAG-mCherry-P

pLV3-CMV-CSF2-3×FLAG-mCherry-Puro plasmid

PVT87833

pLV3-CMV-CSF2-3×FLAG-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-CSF2(human)-3×FLAG-mCherry-Puro
Alias: NM_000758.4;CSF; GMCSF
Gene lenght: 432bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV3-CMV-MCS-3×FLAG-mCherry-Puro
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CSF2-3×FLAG-mCherry-Puro plasmid Description

pLV3-CMV-CSF2-3×FLAG-mCherry-Puro This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a strong CMV promoter that can initiate the transcription of the human CSF2 gene, promoting the C-terminal fusion expression with a 3×FLAG tag, facilitating detection, localization, and purification of the expressed fusion protein using FLAG antibodies. Additionally, the plasmid contains HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, facilitating stable integration of the gene into host cells. The plasmid carries an mCherry red fluorescent protein for detecting the expression and location of the target gene. The plasmid carries a Puro resistance gene for selecting host cells expressing the target gene.

pLV3-CMV-CSF2-3×FLAG-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatgtggct gcagagcctg ctgctcttgg gcactgtggc 2341 ctgcagcatc tctgcacccg cccgctcgcc cagccccagc acgcagccct gggagcatgt 2401 gaatgccatc caggaggccc ggcgtctcct gaacctgagt agagacactg ctgctgagat 2461 gaatgaaaca gtagaagtca tctcagaaat gtttgacctc caggagccga cctgcctaca 2521 gacccgcctg gagctgtaca agcagggcct gcggggcagc ctcaccaagc tcaagggccc 2581 cttgaccatg atggccagcc actacaagca gcactgccct ccaaccccgg aaacttcctg 2641 tgcaacccag attatcacct ttgaaagttt caaagagaac ctgaaggact ttctgcttgt 2701 catccccttt gactgctggg agccagtcca ggagggatcc GACTACAAAG ACCATGACGG 2761 TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT GACAAGtagc ggccgcGaag 2821 gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga 2881 gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa 2941 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta 3001 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca 3061 gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg 3121 ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc 3181 gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc 3241 ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact 3301 ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc 3361 tacgctagac gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga 3421 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga 3481 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa 3541 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa 3601 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg 3661 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga 3721 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc 3781 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat 3841 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg 3901 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc 3961 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat 4021 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta 4081 caagggatct cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc 4141 cggccctatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag 4201 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga 4261 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg 4321 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac 4381 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt 4441 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc 4501 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg 4561 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc 4621 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt 4681 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg 4741 tgcctgaaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 4801 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 4861 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 4921 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 4981 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 5041 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 5101 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg 5161 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 5221 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 5281 gcgtctccgc cttcgccctc agacgagtcg gatctccctt tggccgcctc cccgcctggt 5341 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 5401 ggggggactg gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac 5461 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 5521 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 5581 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 5641 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 5701 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 5761 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 5821 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc 5881 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 5941 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc 6001 agagacggcc caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 6061 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 6121 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 6181 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 6241 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 6301 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 6361 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 6421 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 6481 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 6541 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 6601 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 6661 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 6721 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 6781 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 6841 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 6901 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 6961 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 7021 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 7081 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 7141 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 7201 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 7261 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 7321 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 7381 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 7441 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 7501 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 7561 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 7621 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 7681 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 7741 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 7801 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 7861 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 7921 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 7981 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 8041 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 8101 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 8161 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa 8221 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc 8281 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga 8341 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg 8401 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc 8461 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa 8521 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca 8581 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc 8641 agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.