pLH02 plasmid

pLH02 plasmid

PVT91184

pLH02 plasmid Information

Plasmid name: pLH02
Alias:
Gene lenght:
Host: Wide host
Use(s): Gene editing
Fragment type: CRISPR
Species: Lactobacillus brevis ATCC367
Prokaryotic resistance: Chl
Screening marker:
Fluorescent labeling:
Promoter: Non
Replicon: p15A
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: p15A-R
Copy number: High
Induction:

pLH02 plasmid Description

pLH02 This is a plasmid suitable for gene editing in broad hosts.

pLH02 plasmid Sequence

1 GCGTGTTGCT TTGATTGATA GCCAAAAAGC AGCAGTTGAT AAAGCAATTA CTGATATTGC 61 TGAAAAATTG TAATTTATAA ATAAAAATCA CCTTTTAGAG GTGGTTTTTT TATTTATAAA 121 TTATTCGTTT GATTTCGCTT TCGATAGAAC AATCAAAGCG AGAATAAGGA AGATAAATCC 181 CATAAGGGCG GGAGCAGAAT GTCCGAGACT AATGTAAATT TGTCCACCAA TTAAAGGACC 241 GATAACGCGC TCGAGCCTGA TAGAAACAGA AGCCACTGGA GCACGTTTAA ACAATTTAAA 301 TCTACCGTTC GTATAATGTA TGCTATACGA AGTTATGACA ATGTCTTAGG CGTTAAGGTC 361 GTTTTAGCCG ATGGTCGCGA AGTTAAGTAA GGTACCATGC AGTTTAAATT CGGTCCTCGG 421 GATATGATAA GATTAATAGT TTTAGCTATT AATCTTTTTT TATTTTTATT TAAGAATGGC 481 TTAATAAAGC GGTTACTTTG GATTTTTGTG AGCTTGGACT AGAAAAAAAC TTCACAAAAT 541 GCTATACTAG GTAGATAAAA ATTTAGGAGG CATATCAAAT GAACTTTAAT AAAATTGATT 601 TAGACAATTG GAAGAGAAAA GAGATATTTA ATCATTATTT GAACCAACAA ACGACTTTTA 661 GTATAACCAC AGAAATTGAT ATTAGTGTTT TATACCGAAA CATAAAACAA GAAGGATATA 721 AATTTTACCC TGCATTTATT TTCTTAGTGA CAAGGGTGAT AAACTCAAAT ACAGCTTTTA 781 GAACTGGTTA CAATAGCGAC GGAGAGTTAG GTTATTGGGA TAAGTTAGAG CCACTTTATA 841 CAATTTTTGA TGGTGTATCT AAAACATTCT CTGGTATTTG GACTCCTGTA AAGAATGACT 901 TCAAAGAGTT TTATGATTTA TACCTTTCTG ATGTAGAGAA ATATAATGGT TCGGGGAAAT 961 TGTTTCCCAA AACACCTATA CCTGAAAATG CTTTTTCTCT TTCTATTATT CCATGGACTT 1021 CATTTACTGG GTTTAACTTA AATATCAATA ATAATAGTAA TTACCTTCTA CCCATTATTA 1081 CAGCAGGAAA ATTCATTAAT AAAGGTAATT CAATATATTT ACCGCTATCT TTACAGGTAC 1141 ATCATTCTGT TTGTGATGGT TATCATGCAG GATTGTTTAT GAACTCTATT CAGGAATTGT 1201 CAGATAGGCC TAATGACTGG CTTTTATAAT ATGAGATAAT GCCGACTGTA CTTGGATCCT 1261 AAACGCAATT GATGATTGGT TCGGAAGGCA CGTTAGGAAT CATTACCGAA GTAATCGTTA 1321 AACTGTTGCC GATTCCGCTA GGGACCCATA ACTTCGTATA ATGTATGCTA TACGAACGGT 1381 ACAGACCGGG GTCGACCCTT CAATAGAGTT CTTAACGTTA ATCCGAAAAA AACTAACGTT 1441 AATATTAAAA AATAAGATCC GCTTGTGAAT TATGTATAAT TTGATTAGAC TAAAGAATAG 1501 GAGAAAGTAT GATGATATTT AAAAAACTTT CTCGTTAAGA TAGGTTGTTG GTGAGCATGT 1561 TATATACGGA TGTATCGGTT TCCTTAATGC AAAATTTTGT TGCTATCTTA TTAATTTTTC 1621 TATTATATAG ATATATTCAA AGAAAGATAA CATTTAAACG GATCATATTA GATATTTTAA 1681 TAGCGATTAT TTTTTCAATA TTATATCTGT TTATTTCAGA TGCGTCATTA CTTGTAATGG 1741 TATTAATGCG ATTAGGGTGG CATTTTCATC AACAAAAAGA AAATAAGATA AAAACGACTG 1801 ATACAGCTAA TTTAATTCTA ATTATCGTGA TCCAGTTATT GTTAGTTGCG GTTGGGACTA 1861 TTATTAGTCA GTTTACCATA TCGATTATCA AAAGTGATTT CAGCCAAAAT ATATTGAACA 1921 ATAGTGCAAC AGATATAACT TTATTAGGTA TTTTCTTTGC TGTTTTATTT GACGGCTTGT 1981 TCTTTATATT ATTGAAGAAT AAGCGGACTG AATTACAACA TTTAAATCAA GAAATCATTG 2041 AATTTTCGTT AGAAAAACAA TATTTTATAT TTATATTTAT TTTATTTATA GTAATAGAAA 2101 TTATTTTAGC AGTTGGGAAT CTTCAAGGAG TAACAGCCAC GATATTATTA ACCATTATCA 2161 TTATTTTTTG TGTCCTTATC GGGATGACTT TTTGGCAAGT GATGCTTTTT TTGAAGGCTT 2221 ATTCGATTCG CCAAGAAGCC AATGACCAAT TGGTCCGGAA TCAACAACTT CAAGATTATC 2281 TAGTCAATAT CGAACAGCAG TACACCGAAT TACGGCGATT TAAGCATGAT TATCAAAACA 2341 TCTTATTATC GTTGGAGAGT TTTGCCGAAA AGGGCGATCA GCAACAGTTT AAGGCGTATT 2401 ACCAAGAATT ATTAGCACAA CGGCCAATTC AAAGTGAAAT CCAAGGGGCA GTCATTGCAC 2461 AACTCGACTA CTTGAAAAAT GATCCTATTC GAGGATTAGT CATTCAAAAG TTTTTGGCAG 2521 CCAAACAGGC TGGTGTTACT TTAAAATTCG AAATGACCGA ACCAATCGAA TTAGCAACCG 2581 CTAATCTATT AACGGTTATT CGGATTATCG GTATTTTATT AGACAATGCG ATTGAACAAG 2641 CCGTTCAAGA AACCGATCAA TTGGTGAGTT GTGCTTTCTT ACAATCTGAT GGTTTAATCG 2701 AAATTACGAT TGAAAATACG GCCAGTCAAG TTAAGAATCT CCAAGCATTT TCAGAGTTAG 2761 GCTATTCAAC GAAAGGCGCT GGTCGGGGGA CTGGTTTAGC TAATGTGCAG GATTTGATTG 2821 CCAAACAAAC CAATTTATTC TTAGAAACAC AGATTGAAAA TAGAAAGTTA CGACAGACAT 2881 TGATGATTAC GGAGGAAACT TAATTTGTAT CCCGTTTATT TATTAGAGGA TGATTTACAG 2941 CAACAAGCGA TTTATCAGCA AATTATCGCG AATACGATTA TGATTAACGA ATTTGCAATG 3001 ACTTTAACAT GCGCTGCCAG TGATACTGAG ACATTGTTGG CGGCAATTAA GGATCAGCAA 3061 CGAGGTTTAT TCTTTTTGGA TATGGAAATT GAGGATAACC GCCAAGCCGG TTTAGAAGTG 3121 GCAACTAAGA TTCGGCAGAT GATGCCGTTT GCGCAAATTG TCTTCATTAC AACCCACGAG 3181 GAACTGACAT TATTAACGTT AGAACGAAAA ATAGCGCCTT TAGATTACAT TCTCAAGGAC 3241 CAAACAATGG CTGAAATCAA AAGGCAATTG ATTGATGATC TATTGTTAGC TGAGAAGCAA 3301 AACGAGGCGG CAGCGTATCA CCGAGAAAAT TTATTTAGTT ATAAAATAGG TCCTCGCTTT 3361 TTCTCATTAC CATTAAAGGA AGTTGTTTAT TTATATACTG AAAAAGAAAA TCCGGGTCAT 3421 ATTAATTTGT TAGCCGTTAC CAGAAAGGTT ACTTTTCCAG GAAATTTAAA TGCGCTGGAA 3481 GCCCAATATC CAATGCTCTT TCGGTGTGAT AAAAGTTACT TAGTTAACCT ATCTAATATT 3541 GCCAATTATG ACAGTAAAAC ACGGAGTTTA AAATTTGTAG ATGGCAGTGA GGCAAAAGTC 3601 TCGTTCCGGA AATCACGGGA ACTAGTGGCC AAATTAAAAC AAATGATGTA GCGCCTGCAG 3661 CACGCCAAAT GATCCCAGTA AAAAGCCACC CGCATGGCGG GTGGCTTTTT ATTAGCCCTA 3721 GAAGGGCTTC CCACACGCAT TTCAGCGCCT TAGTGCCTTA GTTTGTGAAT CATAGGTGGT 3781 ATAGTCCCGA AATACCCGTC TAAGGAATTG TCAGATAGGC CTAATGACTG GCTTTTATAA 3841 TATGAGATAA TGCCGACTGT ACTTTTTACA GTCGGTTTTC TAATGTCACT AACCTGCCCC 3901 GTTAGTTGAA GAAGGTTTTT ATATTACAGC TCCAGATCTA CCGGTGGGCC CATATTAACG 3961 TTTAACCGAT AAAGTTGAAC GTTAATATTT TTTTTGCGCA GAAATGGTAA ATTGAAGCAT 4021 AATAGTCTTG TAAGGTATTT AGCTGGCTGG CGTAAAGTAT GCTTTATAAA ATAATATATA 4081 GGAGTATGAT TCCCATGTAT ATTACAGCTC CAGATCTACC GGTGGGCCCA TATTAACGTT 4141 TAACCGATAA AGTTGAACGT TAATATTTTT TTTGCGCAGA AATGGTAAAT TGAAGCATAA 4201 TAGTCTTGTA AGGTATTTAG CTGGCTGGCG TAAAGTATGC TTTATAAAAT AATATATAGG 4261 AGTATGATTC CCATGCATAT TAACGTTTAA CCGATAAAGT TGAACGTTAA TATTTTTTTT 4321 GCGCAGAAAT GGTAAATTGA AGCATAATAG TCTTGTAAGG TATTTAGCTG GCTGGCGTAA 4381 AGTATGCTTT ATAAAATAAT ATATAGGAGT ATGATTCCCA TGGACGTAGT CAAGGTACAC 4441 ACAAGCAGTC GTTTCCAGCC TAAAGCAGTG GTTGCTACTA ATTTAGCTGA ACTAGATGAC 4501 ATTAAATCTG ATCTGTTCAA GGAAGTTCAA TTGTTGGCCG AGAATGATCG ACTTAGCAAT 4561 GATGAGATTG ATCGGCTATA CAGCATCAGC GACGAGCTTG TTGCTTGGTC ACCCAATTTG 4621 GAGGAGGAAG AATAAATGGC TAATGAAGTA GCAGCAACAC AACGCTCATT AGATGCAGAT 4681 GTGCAAGATT CAATTAATCA AATGATGAAC CAAGAGAACG GATTGAAGTT GCCAGCAAAC 4741 TATGCCGTAG GAAACGCCCT CAAGTCGGCA TTCTTTGCAC TAAAGGGAAA TAACGATGGG 4801 GACTTGATTC AAGTGGCTGC TCACATGCCT GAAATGAAGA CTTCAATCGC CAATGCCCTC 4861 ATGGATATGG TGGTTCAAGG ATTAACACCG GCCAAGACCC AAGTGTACTT TATCAGATAT 4921 GACAATCAGG TTAAAATGCA GCGTTCCTAC TTTGGGACAC AGGCGGCGCT AAAGCGACTA 4981 TCTGAGGTTC ACGACTGCTG GGCAAATGTA GTTCATGAAG GTGACGGACT AGAAATCGGT 5041 GCCGAAGATG ATCGTTTGGT CGTAAGGGAT TGGAAGCCGA CGTTGGAGGG ACTCGATAAG 5101 GAAATTAAGT ACGTTTATGC CGTCATTGAG ATGGCTGACG GAACTCACCA GCATACTATC 5161 ATGACCTTCA AACAAATCAA AAACAGCTGG TCACAGACAC GGTCTAAAGG GGCTGTGCAG 5221 AACAAGTTCA GTGATGAGAT GGCCAAACGG ACGGTGCTTA ACCGGGCCGC TAAGAACATT 5281 TTAAACACTT CTGATGATTC AGATTTGGTT GTTGGAGCCA TCAACAATAC TACTTCCAAC 5341 GAGTATGACG ATGATCAAGC AGCCAAAGAT GTGACGCCTA AGAAGGTTAC TGATTTGATC 5401 GGTAATGCGG ACACAGAGGA ACCAACGCCT CCTGAATCTA CTGAACAAAC AGAAACAGTT 5461 GAACCAGCAG AACAAGAAGA ACCGGTTGAT ACAAATACGG ACGAAAGTAG CTCTGGCGAG 5521 ATTCGTTCTA TCGAAAATAT GAAGCCGGGT GAAAAGCAAG ACAAGGACAC TGTCAACGAT 5581 ATTTTGGATG GCCTTGAAGA ATCTGAAAAT CATAAGGGGG ATGGCGACGA TGCAAGCAGC 5641 ACCGAAGAAG GACAGGGTGA ACTCTTCCCA CCAGACGTTC ATTCTAAATT CTGATAATTA 5701 TTACAGTCAG AAGGCCAATA GAAACTTCAT GAGTCCAACT TGGTTTAAGA AGTTCGTTGC 5761 CTGTGAGGCA GAGGCATTAG CTGAATTGAG AGGTGAGTGG GCACCCGATG AGGATAAGAC 5821 CGCTTTATTA GTTGGTAATG ACCTGCACAG CTATTTTGAG TCGTCTGAGG CCCATGAACG 5881 GTTCTTAGAT GCCAACAAGG AAGTGATGTT ATCCAGTCGT GGTAAGACTA AAGGCCAGCT 5941 AAAGAGTGAG TACAAGCAGA CCGACATCAT GATTGACTCG CTCAAAAACG ACAAGACGTT 6001 CACACAGTTA TATCAAGGTG AAAAGGAATC AATCGTTACT GGCGTAATTT CTGGCGTTAA 6061 ATGGATGGGC AAGTTGGACT GTTTGAACTT AGATCGTGGG TATTTCATCG ATCTAAAGAC 6121 AACACAGGAA CTGTCTAAGC GGTTTTGGGA TAGCCGTACC CGTCAGTGGA TACCATTCGT 6181 TCTGAAATAT GACTACCAGC TTCAAATGGC TGTCTATCGC GAGCTAGTCA AGCAGCAGTA 6241 CGGAATCGAG TGTGAACCCT ACATTGTTGC AGTAACAAAG CAAAGTCCGC CGGATAAGGC 6301 GGTTATCACG ATTCCTCATG AGTATATGGA GGATGCCTTG CAACGAATAG ACGAGCAGCT 6361 ACCCCATTTT GAGGATGTAA TTGCTGGTGT ACAGGCCCCT GTTCCATGTG GTAGCTGTGC 6421 TTATTGCCGT GAACATAAGC AGTTGGAAAA CATCATCAGC GTTGATGACT TACTAGATAG 6481 TTAGGAATTC GGTACCCCGG GTTCGAAGGC GCCAAGCTTC AAATTACAGC ACGTGTTGCT 6541 TTGATTGATA GCCAAAAAGC AGCAGTTGAT AAAGCAATTA CTGATATTGC TGAAAAATTG 6601 TAATTTATAA ATAAAAATCA CCTTTTAGAG GTGGTTTTTT TATTTATAAA TTATTCGTTT 6661 GATTTCGCTT TCGATAGAAC AATCAAAGCG AGAATAAGGA AGATAAATCC CATAAGGGCG 6721 GGAGCAGAAT GTCCGAGACT AATTCATGGA TCGATTTTTT ATTAAAACGT CTCAAAATCG 6781 TTTCTGAGAC GTTTTAGCGT TTATTTCGTT TAGTTATCGG CATAATCGTT AAAACAGGCG 6841 TTATCGTAGC GTAAAAGCCC TTGAGCGTAG CGTGGCTTTG CAGCGAAGAT GTTGTCTGTT 6901 AGATTATGAA AGCCGATGAC TGAATGAAAT AATAAGCGCA GCGTCCTTCT ATTTCGGTTG 6961 GAGGAGGCTC AAGGGAGTTT GAGGGAATGA AATTCCCTCA TGGGTTTGAT TTTAAAAATT 7021 GCTTGCAATT TTGCCGAGCG GTAGCGCTGG AAAATTTTTG AAAAAAATTT GGAATTTGGA 7081 AAAAAATGGG GGGAAAGGAA GCGAATTTTG CTTCCGTACT ACGACCCCCC ATTAAGTGCC 7141 ACCCCCCATT AAGTGCCGAG TGCCAATTTT TGTGCCAAAA ACGCTCTATC CCAACTGGCT 7201 CAAGGGTTTG AGGGGTTTTT CAATCGCCAA CGAATCGCCA ACGTTTTCGC CAACGTTTTT 7261 TATAAATCTA TATTTAAGTA GCTTTATTGT TGTTTTTATG ATTACAAAGT GATACACTAA 7321 TTTTATAAAA TTATTTGATT GGAGTTTTTT AAATGGTGAT TTCAGAATCG AAAAAAAGAG 7381 TTATGATTTC TCTGACAAAA GAGCAAGATA AAAAATTAAC AGATATGGCG AAACAAAAAG 7441 GTTTTTCAAA ATCTGCGGTT GCGGCGTTAG CTATAGAAGA ATATGCAAGA AAGGAATCAG 7501 AACAAAAAAA ATAAGCGAAA GCTCGCGTTT TTAGAAGGAT ACGAGTTTTC GCTACTTGTT 7561 TTTGATAAGG TAATTATATC ATGGCTATTA AAAATACTAA AGCTAGAAAT TTTGGATTTT 7621 TATTATATCC TGACTCAATT CCTAATGATT GGAAAGAAAA ATTAGAGAGT TTGGGCGTAT 7681 CTATGGCTGT CAGTCCTTTA CACGATATGG ACGAAAAAAA AGATAAAGAT ACATGGAATA 7741 GTAGTGATGT TATACGAAAT GGAAAGCACT ATAAAAAACC ACACTATCAC GTTATATATA 7801 TTGCACGAAA TCCTGTAACA ATAGAAAGCG TTAGGAACAA GATTAAGCGA AAATTGGGGA 7861 ATAGTTCAGT TGCTCATGTT GAGATACTTG ATTATATCAA AGGTTCATAT GAATATTTGA 7921 CTCATGAATC AAAGGACGCT ATTGCTAAGA ATAAACATAT ATACGACAAA AAAGATATTT 7981 TGAACATTAA TGATTTTGAT ATTGACCGCT ATATAACACT TGATGAAAGC CAAAAAAGAG 8041 AATTGAAGAA TTTACTTTTA GATATAGTGG ATGACTATAA TTTGGTAAAT ACAAAAGATT 8101 TAATGGCTTT TATTCGCCTT AGGGGAGCGG AGTTTGGAAT TTTAAATACG AATGATGTAA 8161 AAGATATTGT TTCAACAAAC TCTAGCGCCT TTAGATTATG GTTTGAGGGC AATTATCAGT 8221 GTGGATATAG AGCAAGTTAT GCAAAGGTTC TTGATGCTGA AACGGGGGAA ATAAAATGAC 8281 AAACAAAGAA AAAGAGTTAT TTGCTGAAAA TGAGGAATTA AAAAAAGAAA TTAAGGACTT 8341 AAAAGAGCGT ATTGAAAGAT ACAGAGAAAT GGAAGTTGAA TTAAGTACAA CAATAGATTT 8401 ATTGAGAGGA GGGATTATTG AATAAATAAA AGCCCCCCTG ACGAAAGTCG CACTGATTGG 8461 TGTATCATTT CGTTTTTCTT TTTGTGCGGA CCTGCAGATG CGATATCATG CGCATGCAAG 8521 CTTATCGATG ATAAGCTGTC AAACATGAGA ATTACAACTT ATATCGTATG GGGCTGACTT 8581 CAGGTGCTAC ATTTGAAGAG ATAAATTGCA CTGAAATCTA GAAATATTTT ATCTGATTAA 8641 TAAGATGATC TTCTTGAGAT CGTTTTGGTC TGCGCGTAAT CTCTTGCTCT GAAAACGAAA 8701 AAACCGCCTT GCAGGGCGGT TTTTCGAAGG TTCTCTGAGC TACCAACTCT TTGAACCGAG 8761 GTAACTGGCT TGGAGGAGCG CAGTCACCAA AACTTGTCCT TTCAGTTTAG CCTTAACCGG 8821 CGCATGACTT CAAGACTAAC TCCTCTAAAT CAATTACCAG TGGCTGCTGC CAGTGGTGCT 8881 TTTGCATGTC TTTCCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG 8941 GACTGAACGG GGGGTTCGTG CATACAGTCC AGCTTGGAGC GAACTGCCTA CCCGGAACTG 9001 AGTGTCAGGC GTGGAATGAG ACAAACGCGG CCATAACAGC GGAATGACAC CGGTAAACCG 9061 AAAGGCAGGA ACAGGAGAGC GCACGAGGGA GCCGCCAGGG GGAAACGCCT GGTATCTTTA 9121 TAGTCCTGTC GGGTTTCGCC ACCACTGATT TGAGCGTCAG ATTTCGTGAT GCTTGTCAGG 9181 GGGGCGGAGC CTATGGAAAA ACGGCTTTGC CGCGGCCCTC TCACTTCCCT GTTAAGTATC 9241 TTCCTGGCAT CTTCCAGGAA ATCTCCGCCC CGTTCGTAAG CCATTTCCGC TCGCCGCAGT 9301 CGAACGACCG AGCGTAGCGA GTCAGTGAGC GAGGAAGCGG AATATATCCT GTATCACATA 9361 TTCTGCTGAC GCACCGGTGC AGCCTTTTTT CTCCTGCCAC ATGAAGCACT TCACTGACAC 9421 CCTCATCAGT GCCAACATAG TAAGCCAGTA TACACTCCGC TAGCGCTGAT GTCCGGCGGT 9481 GCTTTTGCCG TTACGCACCA CCCCGTCAGT AGCTGAACAG GAGGGACA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.