pCMV-HSP90AB1-GST-Neo plasmid

pCMV-HSP90AB1-GST-Neo plasmid

PVT91092

pCMV-HSP90AB1-GST-Neo plasmid Information

Plasmid name: pCMV-HSP90AB1(human)-GST-Neo
Alias: NM_007355.4;D6S182; HSP84; HSP90B; HSPC2; HSPCB;
Gene lenght: 2172bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-HSP90AB1-GST-Neo plasmid Description

pCMV-HSP90AB1-GST-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-HSP90AB1-GST-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CACTAGTCCA GTGTGGTGGA ATTCGCCACC atgcctgagg aagtgcacca tggagaggag 961 gaggtggaga cttttgcctt tcaggcagaa attgcccaac tcatgtccct catcatcaat 1021 accttctatt ccaacaagga gattttcctt cgggagttga tctctaatgc ttctgatgcc 1081 ttggacaaga ttcgctatga gagcctgaca gacccttcga agttggacag tggtaaagag 1141 ctgaaaattg acatcatccc caaccctcag gaacgtaccc tgactttggt agacacaggc 1201 attggcatga ccaaagctga tctcataaat aatttgggaa ccattgccaa gtctggtact 1261 aaagcattca tggaggctct tcaggctggt gcagacatct ccatgattgg gcagtttggt 1321 gttggctttt attctgccta cttggtggca gagaaagtgg ttgtgatcac aaagcacaac 1381 gatgatgaac agtatgcttg ggagtcttct gctggaggtt ccttcactgt gcgtgctgac 1441 catggtgagc ccattggcag gggtaccaaa gtgatcctcc atcttaaaga agatcagaca 1501 gagtacctag aagagaggcg ggtcaaagaa gtagtgaaga agcattctca gttcataggc 1561 tatcccatca ccctttattt ggagaaggaa cgagagaagg aaattagtga tgatgaggca 1621 gaggaagaga aaggtgagaa agaagaggaa gataaagatg atgaagaaaa acccaagatc 1681 gaagatgtgg gttcagatga ggaggatgac agcggtaagg ataagaagaa gaaaactaag 1741 aagatcaaag agaaatacat tgatcaggaa gaactaaaca agaccaagcc tatttggacc 1801 agaaaccctg atgacatcac ccaagaggag tatggagaat tctacaagag cctcactaat 1861 gactgggaag accacttggc agtcaagcac ttttctgtag aaggtcagtt ggaattcagg 1921 gcattgctat ttattcctcg tcgggctccc tttgaccttt ttgagaacaa gaagaaaaag 1981 aacaacatca aactctatgt ccgccgtgtg ttcatcatgg acagctgtga tgagttgata 2041 ccagagtatc tcaattttat ccgtggtgtg gttgactctg aggatctgcc cctgaacatc 2101 tcccgagaaa tgctccagca gagcaaaatc ttgaaagtca ttcgcaaaaa cattgttaag 2161 aagtgccttg agctcttctc tgagctggca gaagacaagg agaattacaa gaaattctat 2221 gaggcattct ctaaaaatct caagcttgga atccacgaag actccactaa ccgccgccgc 2281 ctgtctgagc tgctgcgcta tcatacctcc cagtctggag atgagatgac atctctgtca 2341 gagtatgttt ctcgcatgaa ggagacacag aagtccatct attacatcac tggtgagagc 2401 aaagagcagg tggccaactc agcttttgtg gagcgagtgc ggaaacgggg cttcgaggtg 2461 gtatatatga ccgagcccat tgacgagtac tgtgtgcagc agctcaagga atttgatggg 2521 aagagcctgg tctcagttac caaggagggt ctggagctgc ctgaggatga ggaggagaag 2581 aagaagatgg aagagagcaa ggcaaagttt gagaacctct gcaagctcat gaaagaaatc 2641 ttagataaga aggttgagaa ggtgacaatc tccaatagac ttgtgtcttc accttgctgc 2701 attgtgacca gcacctacgg ctggacagcc aatatggagc ggatcatgaa agcccaggca 2761 cttcgggaca actccaccat gggctatatg atggccaaaa agcacctgga gatcaaccct 2821 gaccacccca ttgtggagac gctgcggcag aaggctgagg ccgacaagaa tgataaggca 2881 gttaaggacc tggtggtgct gctgtttgaa accgccctgc tatcttctgg cttttccctt 2941 gaggatcccc agacccactc caaccgcatc tatcgcatga tcaagctagg tctaggtatt 3001 gatgaagatg aagtggcagc agaggaaccc aatgctgcag ttcctgatga gatcccccct 3061 ctcgagggcg atgaggatgc gtctcgcatg gaagaagtcg atggtggagg cggatcagga 3121 ggcggtggat ctGATATCat gtcccctata ctaggttatt ggaaaattaa gggccttgtg 3181 caacccactc gacttctttt ggaatatctt gaagaaaaat atgaagagca tttgtatgag 3241 cgcgatgaag gtgataaatg gcgaaacaaa aagtttgaat tgggtttgga gtttcccaat 3301 cttccttatt atattgatgg tgatgttaaa ttaacacagt ctatggccat catacgttat 3361 atagctgaca agcacaacat gttgggtggt tgtccaaaag agcgtgcaga gatttcaatg 3421 cttgaaggag cggttttgga tattagatac ggtgtttcga gaattgcata tagtaaagac 3481 tttgaaactc tcaaagttga ttttcttagc aagctacctg aaatgctgaa aatgttcgaa 3541 gatcgtttat gtcataaaac atatttaaat ggtgatcatg taacccatcc tgacttcatg 3601 ttgtatgacg ctcttgatgt tgttttatac atggacccaa tgtgcctgga tgcgttccca 3661 aaattagttt gttttaaaaa acgtattgaa gctatcccac aaattgataa gtacttgaaa 3721 tccagcaagt atatagcatg gcctttgcag ggctggcaag ccacgtttgg tggtggcgac 3781 catcctccaa aatgaGCGGC CGCTCGAGTC TAGAGGGCCC GTTTAAACCC GCTGATCAGC 3841 CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT 3901 GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA 3961 TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA 4021 GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC 4081 GGAAAGAACC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG 4141 CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC 4201 CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC 4261 TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA 4321 AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG 4381 CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC 4441 ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA 4501 TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG 4561 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 4621 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 4681 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 4741 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 4801 ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 4861 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 4921 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 4981 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 5041 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 5101 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 5161 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 5221 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 5281 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 5341 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 5401 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 5461 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 5521 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 5581 GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 5641 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 5701 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 5761 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 5821 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 5881 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 5941 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 6001 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 6061 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 6121 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 6181 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 6241 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 6301 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 6361 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 6421 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 6481 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 6541 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 6601 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 6661 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 6721 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 6781 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 6841 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 6901 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 6961 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 7021 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 7081 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 7141 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 7201 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 7261 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 7321 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 7381 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 7441 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 7501 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 7561 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 7621 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 7681 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 7741 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 7801 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 7861 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 7921 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 7981 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 8041 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 8101 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 8161 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 8221 TTCCCCGAAA AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGT

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.