pLV2-CMV-FBXW11-3×Myc-Puro pla

pLV2-CMV-FBXW11-3×Myc-Puro plasmid

PVT93493

pLV2-CMV-FBXW11-3×Myc-Puro plasmid Information

Plasmid name: pLV2-CMV-FBXW11(human)-3×Myc-Puro
Alias: NM_033645.3;BTRC2; BTRCP2; Fbw11; FBW1B; FBXW1B; Hos;NEDJED
Gene lenght: 1527bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-FBXW11-3×Myc-Puro plasmid Description

pLV2-CMV-FBXW11-3×Myc-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-CMV-FBXW11-3×Myc-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatggagcc cgactcggtg attgaggaca agaccatcga 2881 gctcatgata agtaatggaa catcatctgt gatcgtctcc agaaagaggc catcagaagg 2941 aaactatcaa aaagaaaaag acttgtgtat taaatatttt gaccagtggt ctgaatcaga 3001 tcaagtggaa tttgtggaac atcttatttc acgaatgtgt cattatcagc atggacatat 3061 taactcttac ctgaagccca tgttgcagcg ggactttatt accgctttac cagagcaagg 3121 cttagatcac atagcagaaa acattctttc gtacctggat gccaggtctc tgtgtgcagc 3181 agagctggta tgtaaagaat ggcagcgagt gatctcagaa ggaatgcttt ggaagaagct 3241 gattgaacga atggtacgca ctgatcccct atggaaagga ctttcagaaa gaagagggtg 3301 ggatcagtac ctgtttaaaa acagacccac agatggccct ccaaattcat tttataggtc 3361 attataccca aagattatcc aggatataga gactatagaa tctaactggc ggtgtggacg 3421 acacaacttg cagaggattc agtgccgctc tgaaaatagt aaaggtgtct actgtttaca 3481 gtacgatgat gaaaaaatta tcagtggcct acgagataat tctattaaga tatgggataa 3541 aaccagcctg gaatgtttga aagtgttaac aggacacaca ggctctgtcc tctgtctgca 3601 gtatgatgag cgtgtcattg taactggctc ttcagattct acggtgagag tgtgggatgt 3661 gaacacgggt gaagttctta acacattgat ccaccacaat gaggctgtat tgcacttacg 3721 cttcagcaat ggactgatgg tgacctgttc caaggaccgc tccattgctg tgtgggacat 3781 ggcttctgcg accgacatca ctttacgccg tgtcctggtt ggccaccggg ctgccgtcaa 3841 tgtagtagac tttgacgaca agtacatcgt gtctgcctct ggtgacagga ccatcaaagt 3901 ctggagcacg agcacctgtg aatttgttcg tactctcaat gggcacaagc ggggcattgc 3961 ctgtctccag tacagggatc gcctggttgt tagtggatca tcagataata ccattaggct 4021 ctgggatatt gaatgtggtg cctgtttaag agtcctagag ggacatgaag aattggtccg 4081 atgcatccgg tttgataaca agaggattgt cagtggggcc tatgatggga aaattaaagt 4141 ttgggacttg caagctgctc ttgaccctcg agccccagca agcacattgt gtttgcgcac 4201 attggtggaa cattctggac gtgtgtttcg gctccagttt gatgagtttc agatcatcag 4261 cagctcccat gatgacacta ttttgatttg ggatttctta aatgtgcctc ccagtgccca 4321 gaatgagacc cgttctccct ccagaacata cacttacatc tctagaggat ccGAACAAAA 4381 ACTAATATCA GAGGAAGATT TGGAACAGAA ACTAATCAGT GAAGAAGATT TAGAGCAAAA 4441 GTTAATTTCC GAGGAGGACT TATAGTGAtc tagataattc taccgggtag gggaggcgct 4501 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac 4561 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt 4621 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc 4681 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt 4741 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata 4801 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg 4861 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt 4921 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct 4981 ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc 5041 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 5101 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 5161 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 5221 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 5281 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 5341 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 5401 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 5461 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 5521 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 5581 atgacccgca agcccggtgc ctgaccgcgt ctggaacaat caacctctgg attacaaaat 5641 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 5701 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 5761 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 5821 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 5881 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 5941 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 6001 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct 6061 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 6121 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg 6181 gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa 6241 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa 6301 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg 6361 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg 6421 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc 6481 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt 6541 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga 6601 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa 6661 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat 6721 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac 6781 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata 6841 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga 6901 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 6961 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 7021 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 7081 ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgttttac 7141 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 7201 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 7261 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 7321 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 7381 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 7441 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 7501 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 7561 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 7621 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 7681 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 7741 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 7801 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 7861 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 7921 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 7981 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 8041 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 8101 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 8161 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 8221 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 8281 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 8341 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 8401 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 8461 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 8521 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 8581 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 8641 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 8701 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 8761 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 8821 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 8881 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 8941 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 9001 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 9061 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 9121 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 9181 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 9241 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 9301 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt 9361 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 9421 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 9481 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt 9541 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt 9601 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt ttttaaagaa 9661 attgtatttg ttaaatatgt actacaaact tagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.