pCLDN6-Fluc-SV40-hRluc-Neo pla

pCLDN6-Fluc-SV40-hRluc-Neo plasmid

PVT91074

pCLDN6-Fluc-SV40-hRluc-Neo plasmid Information

Plasmid name: pCLDN6(human)-Fluc-SV40-hRluc-Neo
Alias: -
Gene lenght: 2253bp
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Fluc,Rluc
Promoter: CLDN6
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: cer region-F1
Reverse primer:
Copy number: High
Induction:

pCLDN6-Fluc-SV40-hRluc-Neo plasmid Description

pCLDN6-Fluc-SV40-hRluc-Neo is a plasmid suitable for promoter detection in mammalian cells.

pCLDN6-Fluc-SV40-hRluc-Neo plasmid Sequence

1 CATGCAAGCT GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC 61 CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCGGCCGCT TCGAGCAGAC ATGATAAGAT 121 ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG 181 AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA 241 ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GATGTGGGAG GTTTTTTTAA 301 GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGAATTTTA ACAAAATATT AACGCTTACA 361 ATTTCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATACGCG 421 GATCTGCGCA GCACCATGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT TAGGTACCTT 481 CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG 541 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG 601 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC 661 AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA 721 TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC 781 CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA 841 GCTTGATTCT TCTGACACAA CAGTCTCGAA CCAAAGGCTG GAGCCACCAT GGCTTCCAAG 901 GTGTACGACC CCGAGCAACG CAAACGCATG ATCACTGGGC CTCAGTGGTG GGCTCGCTGC 961 AAGCAAATGA ACGTGCTGGA CTCCTTCATC AACTACTATG ATTCCGAGAA GCACGCCGAG 1021 AACGCCGTGA TTTTTCTGCA TGGTAACGCT GCCTCCAGCT ACCTGTGGAG GCACGTCGTG 1081 CCTCACATCG AGCCCGTGGC TAGATGCATC ATCCCTGATC TGATCGGAAT GGGTAAGTCC 1141 GGCAAGAGCG GGAATGGCTC ATATCGCCTC CTGGATCACT ACAAGTACCT CACCGCTTGG 1201 TTCGAGCTGC TGAACCTTCC AAAGAAAATC ATCTTTGTGG GCCACGACTG GGGGGCTTGT 1261 CTGGCCTTTC ACTACTCCTA CGAGCACCAA GACAAGATCA AGGCCATCGT CCATGCTGAG 1321 AGTGTCGTGG ACGTGATCGA GTCCTGGGAC GAGTGGCCTG ACATCGAGGA GGATATCGCC 1381 CTGATCAAGA GCGAAGAGGG CGAGAAAATG GTGCTTGAGA ATAACTTCTT CGTCGAGACC 1441 ATGCTCCCAA GCAAGATCAT GCGGAAACTG GAGCCTGAGG AGTTCGCTGC CTACCTGGAG 1501 CCATTCAAGG AGAAGGGCGA GGTTAGACGG CCTACCCTCT CCTGGCCTCG CGAGATCCCT 1561 CTCGTTAAGG GAGGCAAGCC CGACGTCGTC CAGATTGTCC GCAACTACAA CGCCTACCTT 1621 CGGGCCAGCG ACGATCTGCC TAAGATGTTC ATCGAGTCCG ACCCTGGGTT CTTTTCCAAC 1681 GCTATTGTCG AGGGAGCTAA GAAGTTCCCT AACACCGAGT TCGTGAAGGT GAAGGGCCTC 1741 CACTTCAGCC AGGAGGACGC TCCAGATGAA ATGGGTAAGT ACATCAAGAG CTTCGTGGAG 1801 CGCGTGCTGA AGAACGAGCA GACCGGTGGT GGGAGCGGAG GTGGCGGATC AGGTGGCGGA 1861 GGCTCCGGAG GGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 1921 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 1981 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 2041 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 2101 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 2161 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 2221 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 2281 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 2341 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 2401 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 2461 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC 2521 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 2581 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 2641 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 2701 CGCCCAACCT GCCATCACGA TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT 2761 GTTGGTTTTT TGTGTGAATC GATAGCGATA ATCTTTGCGC TTGCGTTTTC CCTTGTCCAG 2821 ATAGCCCAGT AGCTGACATT CATCCGGGGT CAGCACCGTT TCTGCGGACT GGCTTTCTAC 2881 GTAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT 2941 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA 3001 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 3061 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 3121 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC 3181 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTC 3241 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT 3301 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 3361 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 3421 ACTGCGGCCA ACTTACTTCT GACAACTATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG 3481 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC 3541 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA 3601 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 3661 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 3721 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT 3781 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA 3841 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ATTCGAAATG 3901 ACCGACCAAG CGACGCCCAA CCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 3961 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 4021 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 4081 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 4141 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 4201 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 4261 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 4321 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 4381 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 4441 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG 4501 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 4561 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 4621 GAAAAAAAGG ATTTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 4681 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 4741 TCCTTTTATA GTCCGGAAAT ACAGGAACGC ACGCTGGATG GCCCTTCGCT GGGATGGTGA 4801 AACCATGAAA AATGGCAGCT TCAGTGGATT AAGTGGGGGT AATGTGGCCT GTACCCTCTG 4861 GTTGCATAGG TATTCATACG GTTAAAATTT ATCAGGCGCG ATTGCGGCAG TTTTTCGGGT 4921 GGTTTGTTGC CATTTTTACC TGTCTGCTGC CGTGATCGCG CTGAACGCGT TTTAGCGGTG 4981 CGTACAATTA AGGGATTATG GTAAATCCAC TTACTGTCTG CCCTCGTAGC CATCGAGATA 5041 AACCGCAGTA CTCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTc gagctcaagc 5101 ttcgaattcG ACGCAGACCC CCTTCCTTGC CCAGCCGGTG ATCTAGTCCA GCGTGCGTGG 5161 TGTGGGGAAA TCAAAAATTA GACACGAGTG CTCGCTTCGG CAGCACATAT AGAAATCAGA 5221 CACGAACGGC CGGACGCGGT GGCTCACGCC TTTAATCCCA TCAGTTTGGG AGTCTGAGGC 5281 AGGCGGATTA CGAGGTCAGG AGTTCGAGAG CAGCCTGACC AACGTGGTGA AACGCCGTCT 5341 CTACTCGAAA TACAAAAATT AGCCGGGCGT GGTGGCGCGC GCCTGCAATC CGAGCTACTC 5401 AGGAGGCTGA GGCAGGAGAA TCTCTTGAAC CCGGGAGGCA GAAGTTGCAG TGAGCCGAGA 5461 TCGCGCCACT ACACTCCAGC CTGGGCGACA GAGTGAGAGT CCGTCTCAAA TAAATAAATA 5521 AATAAATAAA TAAATAAATA AACAAACAAA CAAACAATAA AAAGAAATCA GACACACGAC 5581 CAATCCATGC AGAGATCACT GCAGCTTCCT TCCCCCCACC TCCCCTTGAG ATTGCGTCTC 5641 ACTCTTGCTC AGCCTGGAGT GCAGTGGTAC GATCACGGCT CTCTGGGCTC AGGTGAGCCT 5701 CCCACCTCAG CCTCCCAAGT AGCTGGGACC ACAGGCCCTG CCCCACCACG TCCGGCTAAT 5761 TTTTGTATTT TTTGTAGAGA CCAGGGTCTT ACTATGTTGC CCAGGTTAGC CTGGCCACTA 5821 CAGCTTTGTT AAGGGCCCGG GGGGTATAAC AATTAAACCT GAGGTCAGGA GCTCGAGACC 5881 AGCCTGGCCA ACTTGGTGAA CCCCCGTCTT TACTAAAAAT AAAAAAAAGA AAGAAGTTAG 5941 CCGGGCGTGG TAGTGGGCGC CTGTAATCTC AGCTACTCGG GAGACTGAGG CAGGAGAATT 6001 TCTGGAACCT GGGAGACGGA GGTTGCAGTC AGTCGAGATA GCGCCATTGC ACTCCAGACT 6061 GGGCGACAGA GAGGGCCTCC ATCTCAAAAA ACAAAACAGG CCGGGCGCGG TGGCTCACGC 6121 CTGTAATCCC AGCACATTGG GAGGCCGAGG CGGGCAGATC ACGAGGTCAG GAAATCGAGG 6181 CCATCCTGGC TAACACGGTG AAACCCCGTC TCTACTAAAA ATACAAAAAA ATTAGTCGGG 6241 GGTGGTGGCG GGCACCTGTA GTAGTCCCAG CTACTCTGGA GGCTGAGGCA GGTGAATGGC 6301 GTGAACCCGG AAGGCAGAGC TTGCAGTGAG CCAAGATCGC GCCACCACAC TCCAGCCTTG 6361 GCGAGAGAGC GAGACTCCAT CTCAAAAACA AACAACAACA ACAACAAAAA ACCAGCAACA 6421 TAAAAATAAT ATTATCTGGC CGGGCGCGGT GGCTTAAGCC GGTAATCCCA GCGCAGTGCG 6481 AGGCCAAGGC GGGCGGATCG CTTGAGTCCG GGAGTTCGAG ACGCCTGGGC AATATAACAA 6541 GACCCTGTCT ATACAAAACA AAACATAAAT TAGCTGGGCA CGGTGGCGTG TGCTGCCTGT 6601 AGTCCCAGCT ACTCAGGAGG CTGAGGCGGG AGGATCGCTT GAGCCCAGAA GGTCGAGACT 6661 GCAGTGAGCC GTGATCGCAC CACTGCACTC CAGCCTGGGT GACACAGTGA AAATATTTCT 6721 CTAAAATAAT ACCAATAGTA TGCCCTTTAA TTTGTACCTT AGGACGTAGT TACATAATAA 6781 AATGCAGACT TGTTATCTCC ATTTTGCAGA GTAGGAAACG AAGGCTCGGG AAGGTTAGGT 6841 GAGTTCCCCA TGTCACCGCG CTGCTTAAGT GGTGAAGCGG AGTCTCAAGC TCTGTTCTAC 6901 CCTAAGCCTG GCGCCCTCAC CCTGGGTGAG TCTGGACGTC TGCCAGCCCG AGGCGGGCCC 6961 AGCGACCCCA GCCTCCAGCG CCCCCCTCTG ACAAGCCTGG GATTCGCACG CCCGCTGCCG 7021 TCAGGGACAG GGTCTCTGCC CCTCCTCCGG GGCGGTGGTC CAGTGACGTC ACCGCTTCTT 7081 TAAGACCCCC GCCTCCGCCC CTGTCCCGAC ACTCGGCCTA GGAATTTCCC TTATCTCCTT 7141 CGCAGGTGAG TCCGGGGCTA GTGTGCGTGT TGGAGAGACG CCTCTTGGGG CCGGGTGCAG 7201 GGTGGTGGCG GCGTGGGTAG GGGTGCGTGC GAACTCGGGC TGCGGCCACC TGGATGGGCG 7261 AGTCCCTGAG GGGGTCGGCC GGTCCGCGCC CAAGCCTCCG AGGTGATAGG GTCCTTCGGG 7321 GCTCCCAAGG GTTAAACGCC CCTCCCCCCT CCCCAGGGAC AAggatccCA CCATGGAAGA 7381 TGCCAAAAAC ATTAAGAAGG GCCCAGCGCC ATTCTACCCA CTCGAAGACG GGACCGCCGG 7441 CGAGCAGCTG CACAAAGCCA TGAAGCGCTA CGCCCTGGTG CCCGGCACCA TCGCCTTTAC 7501 CGACGCACAT ATCGAGGTGG ACATTACCTA CGCCGAGTAC TTCGAGATGA GCGTTCGGCT 7561 GGCAGAAGCT ATGAAGCGCT ATGGGCTGAA TACAAACCAT CGGATCGTGG TGTGCAGCGA 7621 GAATAGCTTG CAGTTCTTCA TGCCCGTGTT GGGTGCCCTG TTCATCGGTG TGGCTGTGGC 7681 CCCAGCTAAC GACATCTACA ACGAGCGCGA GCTGCTGAAC AGCATGGGCA TCAGCCAGCC 7741 CACCGTCGTA TTCGTGAGCA AGAAAGGGCT GCAAAAGATC CTCAACGTGC AAAAGAAGCT 7801 ACCGATCATA CAAAAGATCA TCATCATGGA TAGCAAGACC GACTACCAGG GCTTCCAAAG 7861 CATGTACACC TTCGTGACTT CCCATTTGCC ACCCGGCTTC AACGAGTACG ACTTCGTGCC 7921 CGAGAGCTTC GACCGGGACA AAACCATCGC CCTGATCATG AACAGTAGTG GCAGTACCGG 7981 ATTGCCCAAG GGCGTAGCCC TACCGCACCG CACCGCTTGT GTCCGATTCA GTCATGCCCG 8041 CGACCCCATC TTCGGCAACC AGATCATCCC CGACACCGCT ATCCTCAGCG TGGTGCCATT 8101 TCACCACGGC TTCGGCATGT TCACCACGCT GGGCTACTTG ATCTGCGGCT TTCGGGTCGT 8161 GCTCATGTAC CGCTTCGAGG AGGAGCTATT CTTGCGCAGC TTGCAAGACT ATAAGATTCA 8221 ATCTGCCCTG CTGGTGCCCA CACTATTTAG CTTCTTCGCT AAGAGCACTC TCATCGACAA 8281 GTACGACCTA AGCAACTTGC ACGAGATCGC CAGCGGCGGG GCGCCGCTCA GCAAGGAGGT 8341 AGGTGAGGCC GTGGCCAAAC GCTTCCACCT ACCAGGCATC CGCCAGGGCT ACGGCCTGAC 8401 AGAAACAACC AGCGCCATTC TGATCACCCC CGAAGGGGAC GACAAGCCTG GCGCAGTAGG 8461 CAAGGTGGTG CCCTTCTTCG AGGCTAAGGT GGTGGACTTG GACACCGGTA AGACACTGGG 8521 TGTGAACCAG CGCGGCGAGC TGTGCGTCCG TGGCCCCATG ATCATGAGCG GCTACGTTAA 8581 CAACCCCGAG GCTACAAACG CTCTCATCGA CAAGGACGGC TGGCTGCACA GCGGCGACAT 8641 CGCCTACTGG GACGAGGACG AGCACTTCTT CATCGTGGAC CGGCTGAAGA GCCTGATCAA 8701 ATACAAGGGC TACCAGGTAG CCCCAGCCGA ACTGGAGAGC ATCCTGCTGC AACACCCCAA 8761 CATCTTCGAC GCCGGGGTCG CCGGCCTGCC CGACGACGAT GCCGGCGAGC TGCCCGCCGC 8821 AGTCGTCGTG CTGGAACACG GTAAAACCAT GACCGAGAAG GAGATCGTGG ACTATGTGGC 8881 CAGCCAGGTT ACAACCGCCA AGAAGCTGCG CGGTGGTGTT GTGTTCGTGG ACGAGGTGCC 8941 TAAAGGACTG ACCGGCAAGT TGGACGCCCG CAAGATCCGC GAGATTCTCA TTAAGGCCAA 9001 GAAGGGCGGC AAGATCGCCG TGTAATTCTA GTTGTTTAAA CGAGCTCGCT AGCCTCGAGT 9061 CTAGAGTCGA CCTGCAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.