pIG-641 JE-TRAC-BCMA(BB)28z-P2

pIG-641 JE-TRAC-BCMA(BB)28z-P2A plasmid

PVT90981

pIG-641 JE-TRAC-BCMA(BB)28z-P2A plasmid Information

Plasmid name: pIG-641 JE-TRAC-BCMA(BB)28z-P2A
Alias: Plasmid𭜏
Gene lenght: 2103bp
Host: Mammalian cells,Adeno associated virus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Non
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: Forward left ITR
Reverse primer:
Copy number: High
Induction:

pIG-641 JE-TRAC-BCMA(BB)28z-P2A plasmid Description

pIG-641 JE-TRAC-BCMA(BB)28z-P2A A plasmid suitable for protein expression in adeno-associated virus.

pIG-641 JE-TRAC-BCMA(BB)28z-P2A plasmid Sequence

1 AGGACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 61 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 121 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 181 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 241 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 301 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 361 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 421 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 481 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 541 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 601 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 661 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 721 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 781 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 841 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 901 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 961 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 1021 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 1081 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 1141 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 1201 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 1261 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 1321 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 1381 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 1441 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 1501 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 1561 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 1621 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 1681 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 1741 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 1801 GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA 1861 GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC 1921 ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG 1981 CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT 2041 CAGAGCAGAT TGTACTGAGA GTGCACCATA AAATTGTAAA CGTTAATATT TTGTTAAAAT 2101 TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGACCGAA ATCGGCAAAA 2161 TCCCTTATAA ATCAAAAGAA TAGCCCGAGA TAGAGTTGAG TGTTGTTCCA GTTTGGAACA 2221 AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG 2281 GCGATGGCCC ACTACGTGAA CCATCACCCA AATCAAGTTT TTTGGGGTCG AGGTGCCGTA 2341 AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG 2401 CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAAG GCGCTGGCAA 2461 GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG 2521 GCGCGTACTA TGGTTGCTTT GACGTATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG 2581 AAAATACCGC ATCAGGCGCC CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC 2641 CCGGGCAAAG CCCGGGCGTC GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC 2701 GCGCAGAGAG GGAGTGGCCA ACTCCATCAC TAGGGGTTCC TGCGGCCGCT CGGTCCGCTA 2761 GCGATGTAAG GAGCTGCTGT GACTTGCTCA AGGCCTTATA TCGAGTAAAC GGTAGTGCTG 2821 GGGCTTAGAC GCAGGTGTTC TGATTTATAG TTCAAAACCT CTATCAATGA GAGAGCAATC 2881 TCCTGGTAAT GTGATAGATT TCCCAACTTA ATGCCAACAT ACCATAAACC TCCCATTCTG 2941 CTAATGCCCA GCCTAAGTTG GGGAGACCAC TCCAGATTCC AAGATGTACA GTTTGCTTTG 3001 CTGGGCCTTT TTCCCATGCC TGCCTTTACT CTGCCAGAGT TATATTGCTG GGGTTTTGAA 3061 GAAGATCCTA TTAAATAAAA GAATAAGCAG TATTATTAAG TAGCCCTGCA TTTCAGGTTT 3121 CCTTGAGTGG CAGGCCAGGC CTGGCCGTGA ACGTTCACTG AAATCATGGC CTCTTGGCCA 3181 AGATTGATAG CTTGTGCCTG TCCCTGAGTC CCAGTCCATC ACGAGCAGCT GGTTTCTAAG 3241 ATGCTATTTC CCGTATAAAG CATGAGACCG TGACTTGCCA GCCCCACAGA GCCCCGCCCT 3301 TGTCCATCAC TGGCATCTGG ACTCCAGCCT GGGTTGGGGC AAAGAGGGAA ATGAGATCAT 3361 GTCCTAACCC TGGAATTGGA TCCTCTTGTC TTACAGATGG ATCTGGAGCA ACAAACTTCT 3421 CACTACTCAA ACAAGCAGGT GACGTGGAGG AGAATCCCGG CCCCATGAAA TGGAAAGCAC 3481 TCTTTACCGC CGCAATCCTT CAAGCACAGT TGCCAATTAC CGAGGCTGAC ATTGTACTGA 3541 CGCAAAGTCC CCCTAGCTTG GCGATGAGTC TCGGGAAGCG AGCGACGATT AGTTGCCGAG 3601 CTTCTGAAAG TGTCACAATC CTTGGCTCCC ACCTGATCCA TTGGTACCAA CAAAAACCTG 3661 GGCAGCCCCC GACGCTTCTC ATTCAGTTGG CGTCTAACGT GCAAACAGGA GTACCGGCCA 3721 GATTTTCAGG CTCAGGCTCT CGCACCGACT TTACTCTGAC CATCGACCCT GTTGAGGAGG 3781 ATGATGTAGC AGTTTACTAC TGTCTTCAGA GCAGAACCAT TCCTCGCACA TTCGGCGGTG 3841 GAACGAAGTT GGAAATCAAG GGCTCAACAA GTGGGAGTGG GAAGCCCGGC AGCGGGGAGG 3901 GTTCTACTAA AGGCCAAATA CAGTTGGTTC AATCCGGGCC TGAACTGAAA AAGCCGGGAG 3961 AGACCGTGAA AATTTCTTGC AAGGCTAGCG GGTACACTTT TACGGATTAC TCTATTAACT 4021 GGGTTAAGAG GGCACCGGGC AAAGGGCTGA AATGGATGGG CTGGATAAAC ACCGAGACTC 4081 GGGAGCCTGC ATATGCTTAT GATTTCAGAG GTAGATTTGC GTTCTCTTTG GAAACCTCAG 4141 CTTCAACGGC CTACTTGCAG ATTAATAACT TGAAGTACGA AGACACCGCC ACTTACTTCT 4201 GCGCTCTCGA TTACTCATAC GCTATGGATT ACTGGGGCCA GGGCACGTCC GTGACCGTGT 4261 CCAGCGAACA GAAACTTATA TCAGAGGAAG ACCTTGCAAT TGAAGTTATG TATCCTCCTC 4321 CTTACCTAGA CAATGAGAAG AGCAATGGAA CCATTATCCA TGTGAAAGGG AAACACCTTT 4381 GTCCAAGTCC CCTATTTCCC GGACCTTCTA AGCCCTTTTG GGTGCTGGTG GTGGTTGGTG 4441 GAGTCCTGGC TTGCTATAGC TTGCTAGTAA CAGTGGCCTT TATTATTTTC TGGGTGAGGA 4501 GTAAGAGGAG CAGGCTCCTG CACAGTGACT ACATGAACAT GACTCCCCGC CGCCCCGGGC 4561 CCACCCGCAA GCATTACCAG CCCTATGCCC CACCACGCGA CTTCGCAGCC TATCGCTCCA 4621 GAGTGAAGTT CAGCAGGAGC GCAGACGCCC CCGCGTACCA GCAGGGCCAG AACCAGCTCT 4681 ATAACGAGCT CAATCTAGGA CGAAGAGAGG AGTACGATGT TTTGGACAAG AGACGTGGCC 4741 GGGACCCTGA GATGGGGGGA AAGCCGAGAA GGAAGAACCC TCAGGAAGGC CTGTACAATG 4801 AACTGCAGAA AGATAAGATG GCGGAGGCCT ACAGTGAGAT TGGGATGAAA GGCGAGCGCC 4861 GGAGGGGCAA GGGGCACGAT GGCCTTTACC AGGGTCTCAG TACAGCCACC AAGGACACCT 4921 ACGACGCCCT TCACATGCAG GCCCTGCCCC CTCGCGGAAG CGGAGCTACT AACTTCAGCC 4981 TGCTGAAGCA GGCTGGAGAC GTGGAGGAGA ACCCTGGACC CAATATCCAG AACCCTGACC 5041 CTGCCGTGTA CCAGCTGAGA GACTCTAAAT CCAGTGACAA GTCTGTCTGC CTATTCACCG 5101 ATTTTGATTC TCAAACAAAT GTGTCACAAA GTAAGGATTC TGATGTGTAT ATCACAGACA 5161 AAACTGTGCT AGACATGAGG TCTATGGACT TCAAGAGCAA CAGTGCTGTG GCCTGGAGCA 5221 ACAAATCTGA CTTTGCATGT GCAAACGCCT TCAACAACAG CATTATTCCA GAAGACACCT 5281 TCTTCCCCAG CCCAGGTAAG GGCAGCTTTG GTGCCTTCGC AGGCTGTTTC CTTGCTTCAG 5341 GAATGGCCAG GTTCTGCCCA GAGCTCTGGT CAATGATGTC TAAAACTCCT CTGATTGGTG 5401 GTCTCGGCCT TATCCATTGC CACCAAAACC CTCTTTTTAC TAAGAAACAG TGAGCCTTGT 5461 TCTGGCAGTC CAGAGAATGA CACGGGAAAA AAGCAGATGA AGAGAAGGTG GCAGGAGAGG 5521 GCACGTGGCC CAGCCTCAGT CTCTCCAACT GAGTTCCTGC CTGCCTGCCT TTGCTCAGAC 5581 TGTTTGCCCC TTACTGCTCT TCTAGGCCTC ATTCTAAGCC CCTTCTCCAA GTTGACGCGT 5641 GCGGCCGCAG GAACCCCTAG TGATGGAGTT GGCCACTCCC TCTCTGCGCG CTCGCTCGCT 5701 CACTGAGGCC GGGCGACCAA AGGTCGCCCG ACGCCCGGGC GGCCTCAGTG AGCGAGCGAG 5761 CGCGCAGCTG CCTGC

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.