pLV3-CMV-SKIC2-3×FLAG-Puro pla

pLV3-CMV-SKIC2-3×FLAG-Puro plasmid

PVT96961

pLV3-CMV-SKIC2-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-SKIC2(human)-3×FLAG-Puro
Alias: NM_006929.5;170A; DDX13; HLP; SKI2; SKI2W; SKIV2;SKIV2L; SKIV2L1; THES2
Gene lenght: 3738bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SKIC2-3×FLAG-Puro plasmid Description

pLV3-CMV-SKIC2-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SKIC2-3×FLAG-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catgatggag acagagcgac ttgtgctacc ccctccagat 2341 cccctggacc taccccttcg ggccgtggag ctcggatgca cggggcactg ggagctgctg 2401 aacttgcctg gagctccaga gagtagcctt ccccatggcc tccctccttg tgccccagat 2461 ctgcagcaag aagcagaaca gttgtttctg tcatccccag cctggctgcc tctgcatggt 2521 gtggagcact cagcccgaaa atggcagagg aagacggatc cctggtctct tttggctgtc 2581 ctgggagccc cagtcccatc cgacctacag gcccaaagac acccaaccac aggccagata 2641 ctgggttaca aagaggtctt gctggagaac acaaatctct cggctacaac ctccttgtct 2701 cttcgccggc ctccagggcc agcctcccag tccttatggg gaaatccaac tcagtatccc 2761 ttctggccag gggggatgga tgaacccacc ataacagatc tgaacacacg ggaggaggct 2821 gaggaggaga tagactttga gaaagatctt cttactattc cacctggttt caagaaaggc 2881 atggactttg caccaaaaga ttgtccaact ccagctcctg gactactaag ccttagctgt 2941 atgttggagc ctctggattt gggtgggggt gacgaggatg agaatgaggc agtgggacag 3001 ccaggaggtc ccagagggga cactgtttca gcctctccct gcagtgctcc cctggcccga 3061 gcaagcagct tggaagacct agtgttgaag gaagcgtcca cagctgtatc caccccagag 3121 gccccagagc ctccatctca ggagcagtgg gccatccctg tggacgccac ctcccctgtt 3181 ggtgatttct atcgcctcat tccccagcca gccttccagt gggcatttga gccagatgtg 3241 tttcagaaac aggccatcct gcacttggaa cggcatgact ctgtctttgt cgcagctcac 3301 acatctgcag gaaaaacagt tgtggctgaa tatgccattg ccctggccca gaaacacatg 3361 acacgcacca tctacacttc gcccatcaag gccctgagca accagaagtt ccgggacttc 3421 cgaaacacat tcggggatgt ggggctgctc accggggatg tacagctgca tccggaggcc 3481 tcctgcctca tcatgaccac agagatcctt cgctccatgc tgtacagtgg ctcagatgtt 3541 attcgggacc tggagtgggt catctttgat gaggttcact atatcaacga tgtcgagcgt 3601 ggggtcgtgt gggaggaggt gcttatcatg ctacctgacc acgtttctat catccttctg 3661 agtgccaccg tccccaacgc ccttgagttt gctgactgga ttgggcggct gaagcgtcgt 3721 cagatctatg tgattagcac tgtaacccgc cccgtgcccc tggagcacta tcttttcaca 3781 gggaacagct ccaagaccca gggggagctc tttttgttgc tggactcccg aggagccttc 3841 catacaaaag ggtactatgc agctgtggag gccaagaagg agagaatgag caaacacgcc 3901 cagacctttg gggccaagca gcccacacat caggggggcc ctgcacagga ccgcggagtg 3961 tacctgtccc tcctggcctc cctccgcaca cgtgcccagt tgcccgtggt ggtgttcacc 4021 ttctcccggg gccgctgtga tgagcaggcc tcaggcctca cctcccttga cctcaccacc 4081 agttcggaga agagcgagat ccacctcttc ctgcagcgct gccttgctcg cctccgtggc 4141 tctgaccgcc agctgcccca ggtcctgcac atgtcagagc tcctgaatcg cggcctgggt 4201 gtgcaccata gcggcatcct gcccatcctc aaggagatcg tggagatgct cttcagccgt 4261 ggcctggtca aggtcttgtt tgccacagag acctttgcca tgggagtaaa catgcctgct 4321 cgtacagtag tgtttgactc catgcgcaaa cacgatggct ccaccttccg ggacctgctc 4381 cctggggagt atgtgcagat ggcaggccgg gcagggcgga ggggcctgga ccccacaggc 4441 accgttatcc tgctctgcaa gggccgagtg cccgagatgg cagacctgca ccgcatgatg 4501 atggggaagc cgtcccagct gcagtcccag ttccgcctca cgtacactat gatcctcaac 4561 ttgctgcgag tggatgccct cagggtggag gacatgatga agaggagctt ctctgagttt 4621 ccctcccgca aagacagcaa ggcccatgaa caggccctgg ctgaactgac caagaggctg 4681 ggagctttgg aggagcctga catgactggc caactggtcg acctgcctga atattacagc 4741 tggggggagg aactgacaga gacccagcac atgatccagc gacgcatcat ggagtctgtg 4801 aacgggctga agtctctctc agcaggaagg gtggtggttg tgaagaatca ggagcatcac 4861 aacgcattgg gagtgatcct acaggtctcc tcgaactcca ccagcagagt attcacaacc 4921 ctggtcttgt gtgataagcc cttgtcccag gacccacagg acagggggcc agccactgca 4981 gaggtgccct atccagatga cctcgtggga ttcaagctgt tcctgcctga agggccttgt 5041 gaccacaccg tggtcaagct ccagccagga gatatggctg ccatcaccac caaggtgctc 5101 cgggtgaatg gggagaagat cttggaggac ttcagcaaga ggcagcagcc aaaattcaag 5161 aaggatcctc cccttgcagc cgtgaccact gctgtccagg aactgctgcg tctggctcag 5221 gcccacccag ccggacctcc caccctcgac cctgtcaatg acctgcagct caaagatatg 5281 tcagttgtag agggtgggct ccgggcccgg aagctggagg agctgatcca gggggctcag 5341 tgtgtacaca gcccccgttt tcctgcccag tacctgaagc tgcgggagcg aatgcagata 5401 cagaaggaga tggagcggct gcgcttccta ctgtcggatc agtcattgct gctgcttcct 5461 gagtaccatc agcgagtaga ggtgctccga accctgggtt atgtggacga ggcgggcact 5521 gtgaagctgg cagggcgggt ggcttgtgcc atgagcagcc atgagttgct cctcactgag 5581 ctcatgtttg acaatgcact gagcaccctg cggcctgagg agattgctgc cttgctctct 5641 ggcctggtct gccagagccc tggggacgct ggggatcagc tcccaaacac cctcaagcag 5701 ggaatagaac gtgtccgggc tgtggccaag cggattggtg aggtccaggt ggcttgtggc 5761 ctgaaccaga cggtggagga atttgtgggg gagctgaatt ttgggctggt tgaggttgta 5821 tatgagtggg cccggggcat gcccttctcc gagttggcag ggctctcagg gacccctgag 5881 ggcctggtgg tccgctgcat tcagcgcctg gctgagatgt gtcgctcact gcggggggca 5941 gcccgcctgg taggagagcc tgtgctgggt gccaagatgg agacagcggc taccttgcta 6001 cggcgggaca tcgtatttgc ggccagcctc tacacccagG GATCCGACTA CAAAGACCAT 6061 GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA GTAGCGGCCG 6121 CGAAGGATCT GCGATCGCTC CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC 6181 CCCGAGAAGT TGGGGGGAGG GGTCGGCAAT TGAACGGGTG CCTAGAGAAG GTGGCGCGGG 6241 GTAAACTGGG AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA 6301 CCGTATATAA GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA 6361 ACACAGCTGA AGCTTCGAGG GGCTCGCATC TCTCCTTCAC GCGCCCGCCG CCCTACCTGA 6421 GGCCGCCATC CACGCCGGTT GAGTCGCGTT CTGCCGCCTC CCGCCTGTGG TGCCTCCTGA 6481 ACTGCGTCCG CCGTCTAGGT AAGTTTAAAG CTCAGGTCGA GACCGGGCCT TTGTCCGGCG 6541 CTCCCTTGGA GCCTACCTAG ACTCAGCCGG CTCTCCACGC TTTGCCTGAC CCTGCTTGCT 6601 CAACTCTACG TCTTTGTTTC GTTTTCTGTT CTGCGCCGTT ACAGATCCAA GCTGTGACCG 6661 GCGCCTACGC TAGATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT 6721 CCCCCGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC 6781 CGTCGACCCG GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG 6841 CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG 6901 GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC 6961 CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA 7021 CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG 7081 CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT 7141 GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT 7201 CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA 7261 GCCCGGTGCC TGAGTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG 7321 TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA 7381 TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT 7441 GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT 7501 TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC 7561 TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG 7621 CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC 7681 GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG 7741 CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT 7801 GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC 7861 CTCCCCGCCT GGTACCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT CTTAGCCACT 7921 TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAAA TAAGATCTGC 7981 TTTTTGCTTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT 8041 AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT 8101 GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT 8161 GGAAAATCTC TAGCAGTAGT AGTTCATGTC ATCTTATTAT TCAGTATTTA TAACTTGCAA 8221 AGAAATGAAT ATCAGAGAGT GAGAGGAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 8281 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 8341 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGCT CTAGCTATCC CGCCCCTAAC 8401 TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA 8461 GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG 8521 CCTAGACTTT TGCAGAGACG GCCCAAATTC GTAATCATGG TCATAGCTGT TTCCTGTGTG 8581 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC 8641 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT 8701 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG 8761 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT 8821 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 8881 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 8941 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 9001 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 9061 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 9121 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 9181 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 9241 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 9301 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 9361 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG 9421 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 9481 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 9541 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 9601 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 9661 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG 9721 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT 9781 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC 9841 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA 9901 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA 9961 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA 10021 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT 10081 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC 10141 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT 10201 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC 10261 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG 10321 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT 10381 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC 10441 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG 10501 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC 10561 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG 10621 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT 10681 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC 10741 ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA 10801 CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA 10861 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG 10921 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT 10981 ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC CATTCGCCAT TCAGGCTGCG 11041 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG 11101 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG 11161 TAAAACGACG GCCAGTGCCA AGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.