pLV3-CMV-TIMELESS-3×FLAG-Hyg p

pLV3-CMV-TIMELESS-3×FLAG-Hyg plasmid

PVT90745

pLV3-CMV-TIMELESS-3×FLAG-Hyg plasmid Information

Plasmid name: pLV3-CMV-TIMELESS(human)-3×FLAG-Hyg
Alias: NM_003920.5;FASPS4; hTIM; TIM; TIM1
Gene lenght: 3624bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TIMELESS-3×FLAG-Hyg plasmid Description

pLV3-CMV-TIMELESS-3×FLAG-Hyg It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-TIMELESS-3×FLAG-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agaGCCACCA TGGACTTGCA CATGATGAAC TGTGAACTTC TAGCCACATG TAGTGCCCTT 2341 GGGTACTTGG AGGGAGACAC TTACCATAAG GAACCAGATT GCTTAGAGAG CGTGAAGGAT 2401 CTGATCCGCT ATTTGAGGCA TGAGGATGAG ACACGAGATG TGCGGCAGCA GCTGGGGGCA 2461 GCCCAGATCC TACAGAGCGA CCTTCTGCCC ATCCTCACCC AGCACCACCA GGACAAGCCT 2521 CTCTTTGATG CTGTTATCAG ACTGATGGTG AACTTGACAC AACCAGCCTT GCTCTGTTTT 2581 GGCAATCTGC CTAAGGAGCC CAGCTTTCGG CACCATTTTT TGCAGGTGCT AACTTATTTG 2641 CAGGCCTACA AAGAGGCCTT TGCCAGTGAG AAGGCTTTTG GAGTCCTCAG TGAAACCTTG 2701 TATGAGCTGC TGCAGCTGGG CTGGGAGGAA CGGCAGGAGG AAGACAACTT GCTGATTGAA 2761 CGGATCCTAC TGCTGGTCAG AAATATTCTC CATGTCCCAG CTGACCTTGA TCAGGAGAAG 2821 AAGATTGATG ATGACGCCAG TGCCCATGAC CAGCTCCTCT GGGCGATTCA CCTCAGCGGC 2881 CTGGATGACC TGCTCCTCTT TCTGGCCAGC TCGTCTGCTG AGGAGCAATG GAGCCTACAT 2941 GTGCTAGAGA TTGTCTCCCT TATGTTTCGT GACCAGAACC CCGAGCAGCT GGCGGGAGTA 3001 GGGCAGGGAC GCTTAGCTCA GGAGCGGAGT GCAGATTTTG CAGAACTGGA GGTGTTGCGC 3061 CAGCGAGAGA TGGCAGAAAA GAAGACTCGA GCCCTCCAGC GAGGCAACAG GCATTCTCGA 3121 TTTGGGGGCT CCTATATTGT CCAGGGGTTG AAATCCATTG GGGAGAGGGA CCTCATCTTT 3181 CACAAAGGCC TTCACAACCT ACGAAACTAC AGTTCAGATT TGGGAAAGCA GCCGAAAAAG 3241 GTGCCTAAAC GTCGCCAGGC CGCCCGAGAG CTGTCCATTC AGCGCCGTTC TGCCCTCAAT 3301 GTGAGGCTCT TCCTCAGAGA CTTCTGCTCT GAGTTCCTGG AGAACTGTTA CAACCGGCTC 3361 ATGGGATCAG TAAAGGATCA CCTGCTTCGG GAGAAAGCTC AGCAGCATGA TGAGACCTAT 3421 TATATGTGGG CCTTGGCTTT CTTCATGGCC TTCAACCGAG CTGCCTCCTT CCGGCCAGGC 3481 CTGGTTTCTG AGACCCTCAG TGTCCGTACC TTCCACTTCA TTGAGCAGAA CCTCACCAAC 3541 TACTATGAGA TGATGCTGAC TGACCGCAAG GAAGCTGCCT CCTGGGCACG CCGGATGCAC 3601 TTGGCTCTGA AGGCCTATCA GGAGCTGCTG GCAACAGTGA ATGAGATGGA CATATCTCCA 3661 GATGAGGCTG TGAGGGAGAG CAGCCGCATC ATCAAGAACA ATATTTTCTA TGTGATGGAG 3721 TACCGAGAAC TATTCCTGGC ACTTTTTCGA AAGTTTGATG AGAGATGCCA GCCCCGCTCT 3781 TTCCTTCGTG ACCTGGTGGA GACCACCCAC CTCTTCCTCA AAATGTTGGA GCGATTCTGT 3841 CGGAGCCGTG GGAACCTGGT GGTGCAGAAC AAACAAAAGA AGAGAAGGAA GAAGAAGAAG 3901 AAGGTCCTAG ACCAGGCCAT TGTTTCTGGT AATGTCCCAT CTAGCCCAGA AGAAGTGGAG 3961 GCTGTGTGGC CAGCCCTGGC TGAGCAGCTA CAGTGCTGTG CCCAGAATTC TGAGCTCAGC 4021 ATGGACTCCG TGGTTCCCTT TGATGCGGCC TCAGAGGTGC CAGTGGAGGA GCAGCGGGCA 4081 GAAGCTATGG TACGGATCCA AGACTGTCTC CTGGCTGGCC AGGCCCCACA GGCCCTGACT 4141 CTCCTGAGGT CTGCTCGGGA GGTGTGGCCT GAAGGAGATG TGTTTGGCTC TCAAGACATT 4201 TCTCCAGAGG AAGAGATCCA GTTGCTGAAA CAAATCCTCT CTGCTCCACT TCCCCGGCAG 4261 CAGGGCCCAG AGGAACGTGG GGCAGAGGAA GAAGAAGAGG AGGAGGAGGA GGAAGAGGAG 4321 GAGTTGCAAG TGGTCCAGGT GTCGGAGAAA GAATTTAATT TTCTGGACTA CCTGAAACGC 4381 TTTGCATGTT CAACTGTCGT TCGAGCCTAT GTGCTGCTAC TAAGGAGCTA CCAGCAGAAT 4441 AGTGCCCACA CTAACCATTG CATTGTGAAG ATGCTGCACC GGCTGGCCCA TGACCTCAAA 4501 ATGGAAGCCC TACTTTTTCA GCTGTCAGTC TTCTGCCTCT TCAATCGTCT GCTTAGTGAC 4561 CCTGCTGCTG GAGCCTACAA AGAGCTAGTG ACTTTTGCCA AATACATCCT GGGCAAATTT 4621 TTTGCACTGG CTGCAGTCAA CCAAAAAGCC TTTGTGGAGC TGTTGTTCTG GAAGAACACA 4681 GCTGTGGTTC GAGAGATGAC TGAGGGCTAT GGCTCCCTGG ATGACAGGTC TTCCAGTCGC 4741 AGAGCACCTA CATGGAGCCC CGAAGAAGAG GCTCATCTTC GGGAGCTGTA CCTCGCCAAT 4801 AAGGACGTGG AAGGGCAGGA TGTGGTGGAA GCCATCTTGG CCCACCTGAA TACTGTTCCT 4861 CGAACACGCA AGCAGATCAT CCACCATCTG GTACAGATGG GACTGGCTGA CAGTGTCAAG 4921 GACTTCCAAA GGAAAGGAAC CCATATTGTA CTGTGGACGG GGGATCAGGA GTTGGAGCTG 4981 CAGCGGCTTT TTGAGGAATT CCGGGACTCA GATGATGTCC TGGGTCATAT CATGAAGAAT 5041 ATCACAGCCA AACGCTCACG GGCCCGAATA GTGGATAAAC TCTTGGCTCT GGGGCTGGTG 5101 GCTGAGCGGC GGGAGCTGTA CAAGAAACGG CAGAAAAAGT TGGCATCCTC CATCTTGCCA 5161 AATGGAGCGG AGTCCCTGAA AGATTTTTGC CAGGAAGATC TGGAAGAAGA GGAAAACCTG 5221 CCTGAGGAAG ACAGCGAAGA GGAAGAAGAA GGGGGCTCAG AAGCAGAACA AGTCCAGGGT 5281 AGCTTAGTCC TTTCAAATGA AAACCTTGGT CAAAGCCTGC ATCAGGAAGG CTTTTCTATC 5341 CCGCTCCTAT GGCTCCAGAA CTGCCTGATC CGAGCAGCTG ATGATCGGGA AGAGGATGGC 5401 TGCTCCCAGG CCGTTCCATT GGTGCCACTC ACAGAGGAAA ATGAGGAAGC CATGGAAAAC 5461 GAACAGTTTC AGCAGCTGTT GCGCAAGCTA GGGGTTCGGC CCCCTGCCTC TGGGCAGGAA 5521 ACCTTCTGGC GAATTCCAGC CAAGCTGAGT CCTACCCAGC TCCGGAGGGC AGCAGCTTCT 5581 TTGAGTCAAC CAGAGGAGGA ACAGAAGCTG CAGCCAGAGC TGCAGCCTAA AGTCCCTGGA 5641 GAGCAAGGCT CTGATGAGGA GCACTGTAAA GAGCACCGAG CACAAGCCCT GAGGGCCCTC 5701 TTGCTAGCCC ACAAGAAGAA AGCGGGCCTG GCATCCCCAG AGGAGGAAGA CGCTGTTGGT 5761 AAAGAGCCGC TGAAGGCAGC ACCCAAGAAA CGACAATTGC TGGACAGCGA CGAGGAACAG 5821 GAAGAAGATG AGGGCAGGAA CAGAGCACCA GAGTTGGGAG CTCCAGGAAT CCAAAAGAAG 5881 AAACGATACC AGATTGAGGA TGATGAGGAT GACaccggtG ACTACAAAGA CCATGACGGT 5941 GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGTAGcg gccgcaagga 6001 tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga 6061 agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact 6121 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata 6181 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc 6241 tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc 6301 atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt 6361 ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt 6421 ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct 6481 acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta 6541 cgtcgacatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa 6601 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag 6661 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta 6721 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct 6781 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt 6841 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc 6901 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc 6961 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca 7021 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct 7081 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga 7141 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag 7201 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg 7261 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg 7321 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt 7381 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg 7441 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac 7501 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag 7561 ggcaaaggaa taggtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg 7621 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 7681 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 7741 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 7801 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 7861 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 7921 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc 7981 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 8041 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 8101 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 8161 ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact 8221 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc 8281 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct 8341 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt 8401 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt 8461 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa 8521 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat 8581 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 8641 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac 8701 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 8761 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 8821 cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg 8881 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 8941 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9001 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9061 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 9121 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 9181 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 9241 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 9301 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 9361 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 9421 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 9481 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 9541 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 9601 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 9661 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 9721 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 9781 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 9841 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 9901 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 9961 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 10021 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 10081 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 10141 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 10201 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 10261 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 10321 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 10381 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 10441 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 10501 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 10561 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 10621 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 10681 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 10741 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 10801 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 10861 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 10921 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 10981 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 11041 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga 11101 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 11161 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg 11221 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat 11281 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg 11341 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 11401 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 11461 taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.