pCMV-HA-FBXO11-Neo plasmid

pCMV-HA-FBXO11-Neo plasmid

PVT90704

pCMV-HA-FBXO11-Neo plasmid Information

Plasmid name: pCMV-HA-FBXO11(human)-Neo
Alias: NM_001190274.2;FBX11; IDDFBA; PRMT9; UBR6; UG063H01; VIT1
Gene lenght: 2781bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-HA-FBXO11-Neo plasmid Description

pCMV-HA-FBXO11-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-HA-FBXO11-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG 661 AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC GGGACCGATC CAGCCTCCGG 721 ACTCTAGAGC TAGCGTTTAA ACTTAAGCTT GGTACCGAGC TCGGATCCGC CACCATGTAT 781 CCTTACGACG TGCCTGACTA CGCCGGTGGA GGCGGTAGCa actccgtccg agccgccaac 841 cggagaccca ggcgagtgtc gcggccgcgc ccggtgcagc aacagcagca gcagcccccg 901 cagcagccgc cgccgcagcc gccccagcag cagccgcccc agcagcagcc tccgccgccg 961 ccgcagcagc agcagcagca gcagcctccg ccgccgccac cgccgcctcc gccgctgcct 1021 caggagcgga acaacgtcgg cgagcgggat gatgatgtgc ctgcagatat ggttgcagaa 1081 gaatcaggtc ctggtgcaca aaatagtcca taccaacttc gtagaaaaac tcttttgccg 1141 aaaagaacag cgtgtcccac aaagaacagt atggagggcg cctcaacttc aactacagaa 1201 aactttggtc atcgtgcaaa acgtgcaaga gtgtctggaa aatcacaaga tctatcagca 1261 gcacctgctg aacagtatct tcaggagaaa ctgccagatg aagtggttct aaaaatcttc 1321 tcttacttgc tggaacagga tctttgtaga gcagcttgtg tatgtaaacg cttcagtgaa 1381 cttgctaatg atccaatttt gtggaaacga ttatatatgg aagtatttga atatactcgc 1441 cctatgatgc atcctgaacc tggaaaattc taccagatta atccagaaga gtatgaacat 1501 ccaaatccct ggaaagagag tttccagcag ttgtataaag gtgcacatgt aaagccagga 1561 tttgctgaac atttctacag taaccctgca agatataaag gaagagaaaa tatgttgtat 1621 tatgatacta ttgaagatgc ccttggtggg gtacaagagg ctcattttga tggacttatc 1681 tttgttcatt ctggaatata tactgatgaa tggatatata ttgaatctcc aatcaccatg 1741 attggtgcag cacctgggaa agtggcagac aaagttataa ttgaaaacac tagagattca 1801 accttcgttt ttatggaagg ctctgaagat gcttatgttg gatatatgac aataaggttt 1861 aaccctgatg acaaatctgc acaacaccac aatgcacacc actgcttaga gattacagta 1921 aattgtagcc ctattattga tcactgtatc atccgaagta catgtacagt tggttctgca 1981 gtatgtgtta gtggtcaagg agcatgtccc accatcaagc actgtaacat cagtgactgt 2041 gaaaatgttg gactatatat aacagatcat gcacagggaa tatatgagga taatgaaatt 2101 tccaataatg cgttagctgg gatttgggtt aaaaatcatg gaaacccaat tattagacgg 2161 aatcatattc atcatggacg tgatgttggt gtgttcacat ttgatcatgg catgggttac 2221 tttgaaagtt gcaatataca cagaaatagg atagcaggct ttgaagtaaa agcctatgct 2281 aaccctacag tggttcgatg tgaaattcac catgggcaga ctggaggaat atatgtccat 2341 gaaaaaggaa gaggacaatt catagagaat aaaatctatg caaacaactt tgcaggtgta 2401 tggattacct caaatagtga cccaacaata aggggaaatt ctatatttaa tggaaatcaa 2461 ggaggagttt acatctttgg tgatggacga ggccttattg aaggaaatga catttatggc 2521 aatgcattag caggaattca aattaggaca aacagttgtc caattgttcg gcataacaaa 2581 attcatgatg gccagcatgg tgggatttat gtgcatgaaa agggacaagg agtaatagaa 2641 gagaatgaag tttatagtaa cactctagct ggagtctggg tgacaactgg cagcactcca 2701 gtactgagaa gaaaccggat acacagtggc aagcaggttg gtgtttattt ttatgacaat 2761 ggacatggag tgctagaaga caatgatatc tataatcata tgtattcagg ggttcagata 2821 aggactggaa gcaaccccaa aattagacgc aacaaaatct ggggaggaca gaatggtgga 2881 attctagttt ataattctgg tctaggctgt atagaagaca atgaaatatt tgacaatgca 2941 atggctggag tctggattaa gacagatagt aatcctacac taagaagaaa taaaatccat 3001 gatggaagag atggtggcat ctgtatattt aatgggggtc gaggtctcct tgaagaaaat 3061 gatattttca ggaatgctca agcaggtgtt ctcatcagca ctaatagtca tccaatctta 3121 aggaaaaaca gaatatttga tggatttgcc gcaggtattg aaattacaaa tcacgcaact 3181 gcaacactag aaggcaatca gatttttaac aaccggtttg gaggcttatt tttagcatct 3241 ggtgttaatg tgacaatgaa agataacaaa ataatgaaca atcaagatgc catagaaaag 3301 gctgttagta gaggccaatg tttatataaa atatcaagtt ataccagcta tcccatgcat 3361 gatttctaca gatgtcatac ttgtaacacc acagatcgaa atgccatatg tgtgaactgc 3421 attaagaagt gccatcaggg acatgatgta gagtttatta gacatgatag gtttttctgt 3481 gactgtggtg ctggaacact gtctaatcct tgtacattag ctggtgagcc tacacatgat 3541 acagatacac tatatgactc tgctccacct atagaatcta atacattgca gcacaactga 3601 CTCGAGTCTA GAGGGCCCCT TAAGGGTTCG ATCCCTACCG GTTAGTAATG AGTTTGATAT 3661 CTCGACAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT 3721 GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT 3781 TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG 3841 GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC 3901 CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC 3961 CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT 4021 CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG 4081 CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG 4141 GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG 4201 CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCCTGGA 4261 AACGGGGGAG GCTAACTGAA ACACGGAAGG AGACAATACC GGAAGGAACC CGCGCTATGA 4321 CGGCAATAAA AAGACAGAAT AAAACGCACG GGTGTTGGGT CGTTTGTTCA TAAACGCGGG 4381 GTTCGGTCCC AGGGCTGGCA CTCTGTCGAT ACCCCACCGA GACCCCATTG GGGCCAATAC 4441 GCCCGCGTTT CTTCCTTTTC CCCACCCCAC CCCCCAAGTT CGGGTGAAGG CCCAGGGCTC 4501 GCAGCCAACG TCGGGGCGGC AGGCCCTGCC ATAGCAGATC TGCGCAGCTG GGGCTCTAGG 4561 GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 4621 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 4681 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGCAT CCCTTTAGGG 4741 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 4801 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 4861 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 4921 TTTGATTTAT AAGGGATTTT GGGGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 4981 CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC 5041 CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT 5101 GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT 5161 CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG 5221 CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT 5281 CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA 5341 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT 5401 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA 5461 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC 5521 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA 5581 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG 5641 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC 5701 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG 5761 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA 5821 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC 5881 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA 5941 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG 6001 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT 6061 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG 6121 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC 6181 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GCGAAATGAC CGACCAAGCG 6241 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC 6301 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG 6361 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT 6421 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC 6481 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG 6541 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC 6601 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA 6661 TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT 6721 TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC 6781 TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA 6841 AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA 6901 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG 6961 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG 7021 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT 7081 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT 7141 TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC 7201 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT 7261 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT 7321 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC 7381 TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA 7441 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT 7501 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT 7561 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 7621 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA 7681 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC 7741 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT 7801 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC 7861 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT 7921 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA 7981 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG 8041 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT 8101 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC 8161 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT 8221 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC 8281 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC 8341 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA 8401 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC 8461 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA 8521 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT 8581 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 8641 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 8701 GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGTC GACTCTCAGT ACAATCTGCT 8761 CTGATGCCGC ATAGTTAAGC CAGTATCTGC TCCCTGCTTG TGTGTTGGAG GTCGCTGAGT 8821 AGTGCGCGAG CAAAATTTAA GCTACAACAA GGCAAGGCTT GACCGACAAT TGCATGAAGA 8881 ATCTGCTTAG G

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.