pCMV-DDX17-Myc-6×His-Neo plasm

pCMV-DDX17-Myc-6×His-Neo plasmid

PVT90678

pCMV-DDX17-Myc-6×His-Neo plasmid Information

Plasmid name: pCMV-DDX17(human)-Myc-6×His-Neo
Alias: NM_001098504.2;P72; RH70
Gene lenght: 2193bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-DDX17-Myc-6×His-Neo plasmid Description

pCMV-DDX17-Myc-6×His-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-DDX17-Myc-6×His-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACGG GCCCTCTAGA CTCGAGCGGC CGCCACTGTG CTGGATATCT GCAGAATTCg 961 ccaccCTGCC CACCGGCTTT GTAGCCCCGA TTCTCTGTGT TTTGCTCCCG TCTCCGACGA 1021 GAGAGGCGGC GACGGTGGCG TCTGCGACGG GAGACAGCGC GTCGGAGCGA GAGAGCGCTG 1081 CGCCTGCCGC CGCCCCAACA GCGGAGGCGC CGCCGCCATC GGTCGTCACC AGACCGGAGC 1141 CGCAGGCCCT CCCGAGCCCG GCCATCCGTG CCCCGCTCCC AGATCTCTAT CCTTTTGGGA 1201 CCATGCGCGG AGGAGGCTTT GGGGACCGGG ACCGGGATCG TGACCGTGGA GGATTTGGAG 1261 CAAGAGGTGG TGGTGGCCTT CCCCCGAAGA AATTTGGTAA TCCTGGGGAG CGTTTGCGTA 1321 AAAAAAAGTG GGATTTGAGT GAGCTCCCCA AGTTTGAGAA AAATTTTTAT GTGGAACATC 1381 CGGAAGTAGC AAGGCTGACA CCATATGAGG TTGATGAGCT ACGCCGAAAG AAGGAGATTA 1441 CAGTGAGGGG GGGAGATGTT TGTCCTAAAC CCGTGTTTGC CTTCCATCAT GCTAACTTCC 1501 CACAATATGT AATGGATGTG TTGATGGATC AGCACTTTAC AGAACCAACT CCAATTCAGT 1561 GCCAGGGATT TCCGTTGGCT CTTAGTGGCC GGGATATGGT GGGCATTGCT CAGACTGGCT 1621 CTGGGAAGAC GTTGGCGTAT CTCCTGCCTG CAATTGTTCA TATTAACCAC CAGCCATACT 1681 TGGAAAGGGG AGATGGCCCA ATCTGTCTAG TTCTGGCTCC TACCAGAGAG CTTGCCCAGC 1741 AAGTACAGCA GGTGGCCGAT GACTATGGCA AATGTTCTAG ATTGAAGAGT ACTTGTATTT 1801 ATGGAGGTGC TCCTAAAGGT CCCCAGATTC GAGACTTGGA AAGAGGTGTT GAGATCTGCA 1861 TAGCCACTCC TGGACGTCTG ATAGATTTCC TGGAGTCAGG AAAGACAAAT CTTCGCCGAT 1921 GTACTTACCT TGTATTGGAC GAAGCTGACA GAATGCTTGA TATGGGGTTT GAACCCCAGA 1981 TCCGTAAAAT TGTTGACCAA ATCAGGCCTG ATAGGCAGAC ACTGATGTGG AGTGCAACCT 2041 GGCCAAAAGA AGTAAGACAG CTTGCAGAGG ATTTCCTTCG TGATTACACC CAGATCAACG 2101 TAGGCAATCT GGAGTTGAGT GCCAACCACA ACATCCTCCA GATAGTGGAT GTCTGCATGG 2161 AAAGTGAAAA AGACCACAAG TTGATCCAAC TAATGGAAGA AATAATGGCT GAAAAGGAAA 2221 ACAAAACAAT AATATTTGTG GAGACAAAGA GACGCTGTGA TGATCTGACT CGAAGGATGC 2281 GCAGAGATGG TTGGCCAGCT ATGTGTATCC ATGGAGACAA GAGTCAACCA GAAAGAGATT 2341 GGGTACTTAA TGAGTTCCGT TCTGGAAAGG CACCCATCCT TATTGCTACA GATGTAGCCT 2401 CCCGTGGGCT AGATGTGGAA GATGTCAAGT TTGTGATCAA CTATGACTAT CCAAACAGCT 2461 CAGAGGATTA TGTGCACCGT ATTGGCCGAA CAGCCCGTAG CACCAACAAG GGTACCGCCT 2521 ATACCTTCTT CACCCCAGGG AACCTAAAAC AGGCCAGAGA GCTTATCAAA GTGCTGGAAG 2581 AGGCCAATCA GGCTATCAAT CCAAAACTGA TGCAGCTTGT GGACCACAGA GGAGGCGGCG 2641 GAGGCGGGGG TAAGGGTGGT CGTTCTCGTT ACCGGACCAC TTCTTCAGCC AACAATCCCA 2701 ATCTGATGTA TCAGGATGAG TGTGACCGAA GGCTTCGAGG AGTCAAGGAT GGTGGCCGGA 2761 GAGACTCTGC AAGCTATCGG GATCGTAGTG AAACCGATAG AGCTGGTTAT GCTAATGGCA 2821 GTGGCTATGG AAGTCCAAAT TCTGCCTTTG GAGCACAAGC AGGCCAATAC ACCTATGGTC 2881 AAGGCACCTA TGGGGCAGCT GCTTATGGCA CCAGTAGCTA TACAGCTCAA GAATATGGTG 2941 CTGGCACTTA TGGAGCTAGT AGCACCACCT CAACTGGGAG AAGTTCACAG AGCTCTAGCC 3001 AGCAGTTTAG TGGGATAGGC CGGTCTGGGC AGCAGCCACA GCCACTGATG TCACAACAGT 3061 TTGCACAGCC TCCGGGAGCT ACCAATATGA TAGGTTACAT GGGGCAGACT GCCTACCAAT 3121 ACCCTCCTCC TCCTCCCCCT CCTCCTCCTT CACGTAAAAA GCTTGGGCCC GAACAAAAAC 3181 TCATCTCAGA AGAGGATCTG AATAGCGCCG TCGACCATCA TCATCATCAT CATTGAGTTT 3241 AAACGGTCTC CAGCTTAAGT TTAAACCGCT GATCAGCCTC GACTGTGCCT TCTAGTTGCC 3301 AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA 3361 CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA 3421 TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC 3481 ATGCTGGGGA TGCGGTGGGC TCTATGGCTT CTGAGGCGGA AAGAACCAGC TGGGGCTCTA 3541 GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 3601 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT 3661 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG 3721 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT 3781 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 3841 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 3901 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 3961 AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC 4021 CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG 4081 GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA 4141 GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC 4201 CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC 4261 CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG 4321 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG 4381 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA 4441 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT 4501 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT 4561 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG 4621 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT 4681 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC 4741 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC 4801 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA 4861 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG 4921 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT 4981 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG 5041 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC 5101 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA 5161 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG 5221 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC 5281 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG 5341 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT 5401 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC 5461 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG 5521 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC 5581 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA 5641 TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT 5701 TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC 5761 TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA 5821 AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA 5881 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG 5941 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG 6001 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT 6061 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT 6121 TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC 6181 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT 6241 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT 6301 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC 6361 TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA 6421 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT 6481 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT 6541 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 6601 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA 6661 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC 6721 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT 6781 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC 6841 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT 6901 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA 6961 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG 7021 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT 7081 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC 7141 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT 7201 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC 7261 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC 7321 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA 7381 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC 7441 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA 7501 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT 7561 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 7621 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 7681 GACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.