pCMV-BRCA1-3×Myc-Neo plasmid

pCMV-BRCA1-3×Myc-Neo plasmid

PVT90675

pCMV-BRCA1-3×Myc-Neo plasmid Information

Plasmid name: pCMV-BRCA1(human)-3×Myc-Neo
Alias: NM_001407593.1;BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;PPP1R53; PSCP; RNF53
Gene lenght: 5589bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-BRCA1-3×Myc-Neo plasmid Description

pCMV-BRCA1-3×Myc-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-BRCA1-3×Myc-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatgga tttatctgct 721 cttcgcgttg aagaagtaca aaatgtcatt aatgctatgc agaaaatctt agagtgtccc 781 atctgtctgg agttgatcaa ggaacctgtc tccacaaagt gtgaccacat attttgcaaa 841 ttttgcatgc tgaaacttct caaccagaag aaagggcctt cacagtgtcc tttatgtaag 901 aatgatataa ccaaaaggag cctacaagaa agtacgagat ttagtcaact tgttgaagag 961 ctattgaaaa tcatttgtgc ttttcagctt gacacaggtt tggagtatgc aaacagctat 1021 aattttgcaa aaaaggaaaa taactctcct gaacatctaa aagatgaagt ttctatcatc 1081 caaagtatgg gctacagaaa ccgtgccaaa agacttctac agagtgaacc cgaaaatcct 1141 tccttgcagg aaaccagtct cagtgtccaa ctctctaacc ttggaactgt gagaactctg 1201 aggacaaagc agcggataca acctcaaaag acgtctgtct acattgaatt gggatctgat 1261 tcttctgaag ataccgttaa taaggcaact tattgcagtg tgggagatca agaattgtta 1321 caaatcaccc ctcaaggaac cagggatgaa atcagtttgg attctgcaaa aaaggctgct 1381 tgtgaatttt ctgagacgga tgtaacaaat actgaacatc atcaacccag taataatgat 1441 ttgaacacca ctgagaagcg tgcagctgag aggcatccag aaaagtatca gggtagttct 1501 gtttcaaact tgcatgtgga gccatgtggc acaaatactc atgccagctc attacagcat 1561 gagaacagca gtttattact cactaaagac agaatgaatg tagaaaaggc tgaattctgt 1621 aataaaagca aacagcctgg cttagcaagg agccaacata acagatgggc tggaagtaag 1681 gaaacatgta atgataggcg gactcccagc acagaaaaaa aggtagatct gaatgctgat 1741 cccctgtgtg agagaaaaga atggaataag cagaaactgc catgctcaga gaatcctaga 1801 gatactgaag atgttccttg gataacacta aatagcagca ttcagaaagt taatgagtgg 1861 ttttccagaa gtgatgaact gttaggttct gatgactcac atgatgggga gtctgaatca 1921 aatgccaaag tagctgatgt attggacgtt ctaaatgagg tagatgaata ttctggttct 1981 tcagagaaaa tagacttact ggccagtgat cctcatgagg ctttaatatg taaaagtgaa 2041 agagttcact ccaaatcagt agagagtaat attgaagaca aaatatttgg gaaaacctat 2101 cggaagaagg caagcctccc caacttaagc catgtaactg aaaatctaat tataggagca 2161 tttgttactg agccacagat aatacaagag cgtcccctca caaataaatt aaagcgtaaa 2221 aggagaccta catcaggcct tcatcctgag gattttatca agaaagcaga tttggcagtt 2281 caaaagactc ctgaaatgat aaatcaggga actaaccaaa cggagcagaa tggtcaagtg 2341 atgaatatta ctaatagtgg tcatgagaat aaaacaaaag gtgattctat tcagaatgag 2401 aaaaatccta acccaataga atcactcgaa aaagaatctg ctttcaaaac gaaagctgaa 2461 cctataagca gcagtataag caatatggaa ctcgaattaa atatccacaa ttcaaaagca 2521 cctaaaaaga ataggctgag gaggaagtct tctaccaggc atattcatgc gcttgaacta 2581 gtagtcagta gaaatctaag cccacctaat tgtactgaat tgcaaattga tagttgttct 2641 agcagtgaag agataaagaa aaaaaagtac aaccaaatgc cagtcaggca cagcagaaac 2701 ctacaactca tggaaggtaa agaacctgca actggagcca agaagagtaa caagccaaat 2761 gaacagacaa gtaaaagaca tgacagcgat actttcccag agctgaagtt aacaaatgca 2821 cctggttctt ttactaagtg ttcaaatacc agtgaactta aagaatttgt caatcctagc 2881 cttccaagag aagaaaaaga agagaaacta gaaacagtta aagtgtctaa taatgctgaa 2941 gaccccaaag atctcatgtt aagtggagaa agggttttgc aaactgaaag atctgtagag 3001 agtagcagta tttcattggt acctggtact gattatggca ctcaggaaag tatctcgtta 3061 ctggaagtta gcactctagg gaaggcaaaa acagaaccaa ataaatgtgt gagtcagtgt 3121 gcagcatttg aaaaccccaa gggactaatt catggttgtt ccaaagataa tagaaatgac 3181 acagaaggct ttaagtatcc attgggacat gaagttaacc acagtcggga aacaagcata 3241 gaaatggaag aaagtgaact tgatgctcag tatttgcaga atacattcaa ggtttcaaag 3301 cgccagtcat ttgctccgtt ttcaaatcca ggaaatgcag aagaggaatg tgcaacattc 3361 tctgcccact ctgggtcctt aaagaaacaa agtccaaaag tcacttttga atgtgaacaa 3421 aaggaagaaa atcaaggaaa gaatgagtct aatatcaagc ctgtacagac agttaatatc 3481 actgcaggct ttcctgtggt tggtcagaaa gataagccag ttgataatgc caaatgtagt 3541 atcaaaggag gctctaggtt ttgtctatca tctcagttca gaggcaacga aactggactc 3601 attactccaa ataaacatgg acttttacaa aacccatatc gtataccacc actttttccc 3661 atcaagtcat ttgttaaaac taaatgtaag aaaaatctgc tagaggaaaa ctttgaggaa 3721 cattcaatgt cacctgaaag agaaatggga aatgagaaca ttccaagtac agtgagcaca 3781 attagccgta ataacattag agaaaatgtt tttaaagaag ccagctcaag caatattaat 3841 gaagtaggtt ccagtactaa tgaagtgggc tccagtatta atgaaatagg ttccagtgat 3901 gaaaacattc aagcagaact aggtagaaac agagggccaa aattgaatgc tatgcttaga 3961 ttaggggttt tgcaacctga ggtctataaa caaagtcttc ctggaagtaa ttgtaagcat 4021 cctgaaataa aaaagcaaga atatgaagaa gtagttcaga ctgttaatac agatttctct 4081 ccatatctga tttcagataa cttagaacag cctatgggaa gtagtcatgc atctcaggtt 4141 tgttctgaga cacctgatga cctgttagat gatggtgaaa taaaggaaga tactagtttt 4201 gctgaaaatg acattaagga aagttctgct gtttttagca aaagcgtcca gaaaggagag 4261 cttagcagga gtcctagccc tttcacccat acacatttgg ctcagggtta ccgaagaggg 4321 gccaagaaat tagagtcctc agaagagaac ttatctagtg aggatgaaga gcttccctgc 4381 ttccaacact tgttatttgg taaagtaaac aatatacctt ctcagtctac taggcatagc 4441 accgttgcta ccgagtgtct gtctaagaac acagaggaga atttattatc attgaagaat 4501 agcttaaatg actgcagtaa ccaggtaata ttggcaaagg catctcagga acatcacctt 4561 agtgaggaaa caaaatgttc tgctagcttg ttttcttcac agtgcagtga attggaagac 4621 ttgactgcaa atacaaacac ccaggatcct ttcttgattg gttcttccaa acaaatgagg 4681 catcagtctg aaagccaggg agttggtctg agtgacaagg aattggtttc agatgatgaa 4741 gaaagaggaa cgggcttgga agaaaataat caagaagagc aaagcatgga ttcaaactta 4801 ggtgaagcag catctgggtg tgagagtgaa acaagcgtct ctgaagactg ctcagggcta 4861 tcctctcaga gtgacatttt aaccactcag cagagggata ccatgcaaca taacctgata 4921 aagctccagc aggaaatggc tgaactagaa gctgtgttag aacagcatgg gagccagcct 4981 tctaacagct acccttccat cataagtgac tcttctgccc ttgaggacct gcgaaatcca 5041 gaacaaagca catcagaaaa agcagtatta acttcacaga aaagtagtga ataccctata 5101 agccagaatc cagaaggcct ttctgctgac aagtttgagg tgtctgcaga tagttctacc 5161 agtaaaaata aagaaccagg agtggaaagg tcatcccctt ctaaatgccc atcattagat 5221 gataggtggt acatgcacag ttgctctggg agtcttcaga atagaaacta cccatctcaa 5281 gaggagctca ttaaggttgt tgatgtggag gagcaacagc tggaagagtc tgggccacac 5341 gatttgacgg aaacatctta cttgccaagg caagatctag agggaacccc ttacctggaa 5401 tctggaatca gcctcttctc tgatgaccct gaatctgatc cttctgaaga cagagcccca 5461 gagtcagctc gtgttggcaa cataccatct tcaacctctg cattgaaagt tccccaattg 5521 aaagttgcag aatctgccca gagtccagct gctgctcata ctactgatac tgctgggtat 5581 aatgcaatgg aagaaagtgt gagcagggag aagccagaat tgacagcttc aacagaaagg 5641 gtcaacaaaa gaatgtccat ggtggtgtct ggcctgaccc cagaagaatt tatgctcgtg 5701 tacaagtttg ccagaaaaca ccacatcact ttaactaatc taattactga agagactact 5761 catgttgtta tgaaaacaga tgctgagttt gtgtgtgaac ggacactgaa atattttcta 5821 ggaattgcgg gaggaaaatg ggtagttagc tatttctggg tgacccagtc tattaaagaa 5881 agaaaaatgc tgaatgagca tgattttgaa gtcagaggag atgtggtcaa tggaagaaac 5941 caccaaggtc caaagcgagc aagagaatcc caggacagaa agatcttcag ggggctagaa 6001 atctgttgct atgggccctt caccaacatg cccacagatc aactggaatg gatggtacag 6061 ctgtgtggtg cttctgtggt gaaggagctt tcatcattca cccttggcac aggtgtccac 6121 ccaattgtgg ttgtgcagcc agatgcctgg acagaggaca atggcttcca tgcaattggg 6181 cagatgtgtg aggcacctgt ggtgacccga gagtgggtgt tggacagtgt agcactctac 6241 cagtgccagg agctggacac ctacctgata ccccagatcc cccacagcca ctacCTCGAG 6301 GAACAAAAAC TAATATCAGA GGAAGATTTG GAACAGAAAC TAATCAGTGA AGAAGATTTA 6361 GAGCAAAAGT TAATTTCCGA GGAGGACTTA TAGTGAGGGC CCGATATCTC GACAATCAAC 6421 CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA 6481 CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT 6541 TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG 6601 TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG 6661 GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA 6721 CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA 6781 CTGACAATTC CGTGGTGTTG TCGGGGAAGC TGACGTCCTT TCCATGGCTG CTCGCCTGTG 6841 TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG 6901 CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC 6961 GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCCTGGAAAC GGGGGAGGCT 7021 AACTGAAACA CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG 7081 ACAGAATAAA ACGCACGGGT GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG 7141 GCTGGCACTC TGTCGATACC CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT 7201 CCTTTTCCCC ACCCCACCCC CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG 7261 GGGCGGCAGG CCCTGCCATA GCAGATCTGC GCAGCTGGGG CTCTAGGGGG TATCCCCACG 7321 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 7381 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 7441 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGCATCCC TTTAGGGTTC CGATTTAGTG 7501 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 7561 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 7621 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 7681 GGATTTTGGG GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 7741 CGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC 7801 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC 7861 AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT 7921 CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC 7981 CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT 8041 ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG 8101 AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT 8161 TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA 8221 TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA 8281 GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA 8341 CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA 8401 CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT 8461 CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG 8521 GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA 8581 GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA 8641 TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA 8701 GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG 8761 CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC 8821 GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT 8881 GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA 8941 GTTCTTCTGA GCGGGACTCT GGGGTTCGCG AAATGACCGA CCAAGCGACG CCCAACCTGC 9001 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 9061 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 9121 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 9181 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 9241 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 9301 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 9361 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 9421 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 9481 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 9541 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 9601 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 9661 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 9721 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 9781 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 9841 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC 9901 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 9961 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 10021 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 10081 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA 10141 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 10201 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 10261 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 10321 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 10381 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 10441 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 10501 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 10561 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 10621 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 10681 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 10741 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 10801 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 10861 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 10921 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 10981 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 11041 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 11101 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 11161 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 11221 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 11281 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 11341 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 11401 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT 11461 CGGGAGATCT CCCGATCCCC TATGGTCGAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA 11521 GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT GCGCGAGCAA 11581 AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC TGCTTAGG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.