pMIGR1-MSCV-hCD19-BBz plasmid

pMIGR1-MSCV-hCD19-BBz plasmid

PVT93027

pMIGR1-MSCV-hCD19-BBz plasmid Information

Plasmid name: pMIGR1-MSCV-hCD19-BBz
Alias:
Gene lenght: 1754bp
Host: Mammalian cells,Retrovirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Non
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: PLXSN-F
Reverse primer:
Copy number: High
Induction:

pMIGR1-MSCV-hCD19-BBz plasmid Description

pMIGR1-MSCV-hCD19-BBz is a plasmid suitable for use in mammalian cells, and can also be used for retrovirus packaging.

pMIGR1-MSCV-hCD19-BBz plasmid Sequence

1 AGCCCTTTGT ACACCCTAAG CCTCCGCCTC CTCTTCCTCC ATCCGCCCCG TCTCTCCCCC 61 TTGAACCTCC TCGTTCGACC CCGCCTCGAT CCTCCCTTTA TCCAGCCCTC ACTCCTTCTC 121 TAGGCGCCGC GCCGgaattc accggtgcca ccatggcctc accgttgacc cgctttctgt 181 cgctgaacct gctgctgctg ggtgagtcga ttatcctggg gagtggagaa gctatggccc 241 tgcccgtcac cgctctgctg ctgccccttg ctctgcttct tcatgcagca aggccggaca 301 tccagatgac ccaaaccacc tcatccctct ctgcctctct tggagacagg gtgaccattt 361 cttgtcgcgc cagccaggac atcagcaagt atctgaactg gtatcagcag aagccggacg 421 gaaccgtgaa gctcctgatc taccatacct ctcgcctgca tagcggcgtg ccctcacgct 481 tctctggaag cggatcagga accgattatt ctctcactat ttcaaatctt gagcaggaag 541 atattgccac ctatttctgc cagcagggta ataccctgcc ctacaccttc ggaggaggga 601 ccaagctcga aatcaccggt ggaggaggca gcggcggtgg agggtctggt ggaggtggtt 661 ctgaggtgaa gctgcaagaa tcaggccctg gacttgtggc cccttcacag tccctgagcg 721 tgacttgcac cgtgtccgga gtctccctgc ccgactacgg agtgtcatgg atcagacaac 781 ctccacggaa aggactggaa tggctcggtg tcatctgggg tagcgaaact acttactaca 841 attcagccct caaaagcagg ctgactatta tcaaggacaa cagcaagtcc caagtctttc 901 ttaagatgaa ctcactccag actgacgaca ccgcaatcta ctattgtgct aagcactact 961 actacggagg atcctacgct atggattact ggggacaagg tacttccgtc actgtctctt 1021 caGACTACAA GGATGACGAT GACAAGGATT ACAAAGACGA CGATGATAAG GACTATAAGG 1081 ATGATGACGA CAAAactact accaagccag tgctgcgaac tccctcacct gtgcacccta 1141 ccgggacatc tcagccccag agaccagaag attgtcggcc ccgtggctca gtgaagggga 1201 ccggattgga cttcgcctgt gatatttaca tctgggcacc cttggccgga atctgcgtgg 1261 cccttctgct gtccttgatc atcactctca tctgctacca cTCTGTGCTC AAATGGATCA 1321 GGAAAAAATT CCCCCACATA TTCAAGCAAC CATTTAAGAA GACCACTGGA GCAGCTCAAG 1381 AGGAAGATGC TTGTAGCTGC CGATGTCCAC AGGAAGAAGA AGGAGGAGGA GGAGGCTATG 1441 AGCTGagagc aaaattcagc aggagtgcag agactgctgc caacctgcag gaccccaacc 1501 agctctacaa tgaActcaat ctagggcgaa gagaggaata tgacgtcttg gagaagaagc 1561 gggctcgCga tccagagatg ggaggcaaac agcagaggag gaggaacccc caggaaggcg 1621 tatacaatgc actgcagaaa gacaagatgg cagaagccta cagtgagatc ggcacaaaag 1681 gcgagaggcg gagaggcaag gggcacgatg gcctttacca gggtctcagc actgccacca 1741 aggacaccta tgatgccctg caCatgcaga ccctggcccc tcgctaaGtc gacctgcagg 1801 catgcaagct tcaggtagcc ggctaacgtt aacaaccggt acctctagaa ctatagctag 1861 catgcgcaaa tttaaagcgc tgaTATCGAT AAAATAAAAG ATTTTATTTA GTCTCCAGAA 1921 AAAGGGGGGA ATGAAAGACC CCACCTGTAG GTTTGGCAAG CTAGCTTAAG TAACGCCATT 1981 TTGCAAGGCA TGGAAAATAC ATAACTGAGA ATAGAGAAGT TCAGATCAAG GTTAGGAACA 2041 GAGAGACAGC AGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT 2101 CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCCGCCC TCAGCAGTTT CTAGAGAACC 2161 ATCAGATGTT TCCAGGGTGC CCCAAGGACC TGAAATGACC CTGTGCCTTA TTTGAACTAA 2221 CCAATCAGTT CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA 2281 GCCCACAACC CCTCACTCGG CGCGCCAGTC CTCCGATAGA CTGCGTCGCC CGGGTACCCG 2341 TGTATCCAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG GTCTCGCTGT TCCTTGGGAG 2401 GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT TTCATGGGTA ACAGTTTCTT 2461 GAAGTTGGAG AACAACATTC TGAGGGTAGG AGTCGAATAT TAAGTAATCC TGACTCAATT 2521 AGCCACTGTT TTGAATCCAC ATACTCCAAT ACTCCTGAAA TAGTTCATTA TGGACAGCGC 2581 AGAAAGAGCT GGGGAGAATT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA 2641 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT 2701 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA 2761 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC 2821 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG 2881 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC 2941 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC 3001 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA 3061 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC 3121 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT 3181 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG 3241 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC 3301 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA 3361 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT 3421 AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT 3481 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG 3541 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG 3601 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA 3661 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA 3721 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG 3781 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA 3841 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG 3901 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT 3961 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT 4021 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC 4081 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA 4141 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT 4201 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC 4261 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA 4321 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA 4381 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA 4441 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT 4501 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC 4561 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA 4621 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 4681 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC 4741 TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT 4801 CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG 4861 GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG 4921 GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA 4981 GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG 5041 CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG 5101 CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA 5161 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCGCAAG GAATGGTGCA TGCAAGGAGA 5221 TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC 5281 TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC 5341 CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGCGAT 5401 TAGTCCAATT TGTTAAAGAC AGGATATCAG TGGTCCAGGC TCTAGTTTTG ACTCAACAAT 5461 ATCACCAGCT GAAGCCTATA GAGTACGAGC CATAGATAAA ATAAAAGATT TTATTTAGTC 5521 TCCAGAAAAA GGGGGGAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA 5581 CGCCATTTTG CAAGGCATGG AAAATACATA ACTGAGAATA GAGAAGTTCA GATCAAGGTT 5641 AGGAACAGAG AGACAGCAGA ATATGGGCCA AACAGGATAT CTGTGGTAAG CAGTTCCTGC 5701 CCCGGCTCAG GGCCAAGAAC AGATGGTCCC CAGATGCGGT CCCGCCCTCA GCAGTTTCTA 5761 GAGAACCATC AGATGTTTCC AGGGTGCCCC AAGGACCTGA AATGACCCTG TGCCTTATTT 5821 GAACTAACCA ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC CCGAGCTCAA 5881 TAAAAGAGCC CACAACCCCT CACTCGGCGC GCCAGTCCTC CGATAGACTG CGTCGCCCGG 5941 GTACCCGTAT TCCCAATAAA GCCTCTTGCT GTTTGCATCC GAATCGTGGA CTCGCTGATC 6001 CTTGGGAGGG TCTCCTCAGA TTGATTGACT GCCCACCTCG GGGGTCTTTC ATTTGGAGGT 6061 TCCACCGAGA TTTGGAGACC CCTGCCCAGG GACCACCGAC CCCCCCGCCG GGAGGTAAGC 6121 TGGCCAGCGG TCGTTTCGTG TCTGTCTCTG TCTTTGTGCG TGTTTGTGCC GGCATCTAAT 6181 GTTTGCGCCT GCGTCTGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG 6241 GAACTGACGA GTTCTGAACA CCCGGCCGCA ACCCTGGGAG ACGTCCCAGG GACTTTGGGG 6301 GCCGTTTTTG TGGCCCGACC TGAGGAAGGG AGTCGATGTG GAATCCGACC CCGTCAGGAT 6361 ATGTGGTTCT GGTAGGAGAC GAGAACCTAA AACAGTTCCC GCCTCCGTCT GAATTTTTGC 6421 TTTCGGTTTG GAACCGAAGC CGCGCGTCTT GTCTGCTGCA GCGCTGCAGC ATCGTTCTGT 6481 GTTGTCTCTG TCTGACTGTG TTTCTGTATT TGTCTGAAAA TTAGGGCCAG ACTGTTACCA 6541 CTCCCTTAAG TTTGACCTTA GGTCACTGGA AAGATGTCGA GCGGATCGCT CACAACCAGT 6601 CGGTAGATGT CAAGAAGAGA CGTTGGGTTA CCTTCTGCTC TGCAGAATGG CCAACCTTTA 6661 ACGTCGGATG GCCGCGAGAC GGCACCTTTA ACCGAGACCT CATCACCCAG GTTAAGATCA 6721 AGGTCTTTTC ACCTGGCCCG CATGGACACC CAGACCAGGT CCCCTACATC GTGACCTGGG 6781 AAGCCTTGGC TTTTGACCCC CCTCCCTGGG TCA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.