pLV2-MDH1-HA-Puro plasmid

pLV2-MDH1-HA-Puro plasmid

PVT90665

pLV2-MDH1-HA-Puro plasmid Information

Plasmid name: pLV2-MDH1(human)-HA-Puro
Alias: NM_005917.4;DEE88; EIEE88; HEL-S-32; KAR; MDH-s; MDHA;MGC:1375; MOR2
Gene lenght: 1002bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-MDH1-HA-Puro plasmid Description

pLV2-MDH1-HA-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-MDH1-HA-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatgtctga accaatcaga gtccttgtga ctggagcagc 2881 tggtcaaatt gcatattcac tgctgtacag tattggaaat ggatctgtct ttggtaaaga 2941 tcagcctata attcttgtgc tgttggatat cacccccatg atgggtgtcc tggacggtgt 3001 cctaatggaa ctgcaagact gtgcccttcc cctcctgaaa gatgtcatcg caacagataa 3061 agaagacgtt gccttcaaag acctggatgt ggccattctt gtgggctcca tgccaagaag 3121 ggaaggcatg gagagaaaag atttactgaa agcaaatgtg aaaatcttca aatcccaggg 3181 tgcagcctta gataaatacg ccaagaagtc agttaaggtt attgttgtgg gtaatccagc 3241 caataccaac tgcctgactg cttccaagtc agctccatcc atccccaagg agaacttcag 3301 ttgcttgact cgtttggatc acaaccgagc taaagctcaa attgctctta aacttggtgt 3361 gactgctaat gatgtaaaga atgtcattat ctggggaaac cattcctcga ctcagtatcc 3421 agatgtcaac catgccaagg tgaaattgca aggaaaggaa gttggtgttt atgaagctct 3481 gaaagatgac agctggctca agggagaatt tgtcacgact gtgcagcagc gtggcgctgc 3541 tgtcatcaag gctcgaaaac tatccagtgc catgtctgct gcaaaagcca tctgtgacca 3601 cgtcagggac atctggtttg gaaccccaga gggagagttt gtgtccatgg gtgttatctc 3661 tgatggcaac tcctatggtg ttcctgatga tctgctctac tcattccctg ttgtaatcaa 3721 gaataagacc tggaagtttg ttgaaggtct ccctattaat gatttctcac gtgagaagat 3781 ggatcttact gcaaaggaac tgacagaaga aaaagaaagt gcttttgaat ttctttcctc 3841 tgccggatcc TACCCATACG ACGTACCAGA TTACGCTtag tctagataat tctaccgggt 3901 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac 3961 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca 4021 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa 4081 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct 4141 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg 4201 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg 4261 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg 4321 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc 4381 atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg 4441 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc 4501 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag 4561 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 4621 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 4681 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 4741 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 4801 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 4861 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 4921 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 4981 cgcacctggt gcatgacccg caagcccggt gcctgaccgc gtctggaaca atcaacctct 5041 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 5101 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 5161 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 5221 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 5281 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 5341 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 5401 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc 5461 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 5521 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 5581 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga 5641 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt 5701 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct 5761 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg 5821 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac 5881 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat 5941 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag 6001 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac 6061 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc 6121 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt 6181 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc 6241 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat 6301 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 6361 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 6421 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 6481 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg 6541 ctagcgtttt accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc 6601 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 6661 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 6721 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 6781 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 6841 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 6901 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 6961 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 7021 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 7081 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 7141 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 7201 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 7261 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 7321 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 7381 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 7441 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 7501 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 7561 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 7621 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 7681 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 7741 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 7801 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 7861 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 7921 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 7981 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 8041 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 8101 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 8161 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 8221 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 8281 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 8341 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 8401 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 8461 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 8521 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 8581 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 8641 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 8701 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc 8761 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 8821 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 8881 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc 8941 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc 9001 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta 9061 gtttttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.