pU6-Negative-sgRNA-control-Cas

pU6-Negative-sgRNA-control-Cas9-Puro plasmid

PVT90664

pU6-Negative-sgRNA-control-Cas9-Puro plasmid Information

Plasmid name: pU6-Negative-sgRNA-control-Cas9-Puro
Alias: -
Gene lenght:
Host: Mammalian cells
Use(s): Gene editing
Fragment type: CRISPR
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pU6-Negative-sgRNA-control-Cas9-Puro plasmid Description

pU6-Negative-sgRNA-control-Cas9-Puro is a plasmid suitable for gene editing in mammalian cells.

pU6-Negative-sgRNA-control-Cas9-Puro plasmid Sequence

1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag 61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga 121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat 181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt GTGGAAAGGA 241 CGAAACACCg gCGCTTCCGC GGCCCGTTCA Agttttagag ctaGAAAtag caagttaaaa 301 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcTTT TTTgttttag 361 agctagaaat agcaagttaa aataaggcta gtccgtTTTT agcgcgtgcg ccaattctgc 421 agacaaatgg ctctagaggt acccgttaca taacttacgg taaatggccc gcctggctga 481 ccgcccaacg acccccgccc attgacgtca atagtaacgc caatagggac tttccattga 541 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 601 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattGtgcc 661 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 721 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc 781 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg 841 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg 901 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg 961 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg 1021 acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg 1081 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa 1141 ttagctgagc aagaggtaag ggtttaaggg atggttggtt ggtggggtat taatgtttaa 1201 ttacctggag cacctgcctg aaatcacttt ttttcaggtt GGaccggtgc caccATGGAC 1261 TATAAGGACC ACGACGGAGA CTACAAGGAT CATGATATTG ATTACAAAGA CGATGACGAT 1321 AAGATGGCCC CAAAGAAGAA GCGGAAGGTC GGTATCCACG GAGTCCCAGC AGCCGACAAG 1381 AAGTACAGCA TCGGCCTGGA CATCGGCACC AACTCTGTGG GCTGGGCCGT GATCACCGAC 1441 GAGTACAAGG TGCCCAGCAA GAAATTCAAG GTGCTGGGCA ACACCGACCG GCACAGCATC 1501 AAGAAGAACC TGATCGGAGC CCTGCTGTTC GACAGCGGCG AAACAGCCGA GGCCACCCGG 1561 CTGAAGAGAA CCGCCAGAAG AAGATACACC AGACGGAAGA ACCGGATCTG CTATCTGCAA 1621 GAGATCTTCA GCAACGAGAT GGCCAAGGTG GACGACAGCT TCTTCCACAG ACTGGAAGAG 1681 TCCTTCCTGG TGGAAGAGGA TAAGAAGCAC GAGCGGCACC CCATCTTCGG CAACATCGTG 1741 GACGAGGTGG CCTACCACGA GAAGTACCCC ACCATCTACC ACCTGAGAAA GAAACTGGTG 1801 GACAGCACCG ACAAGGCCGA CCTGCGGCTG ATCTATCTGG CCCTGGCCCA CATGATCAAG 1861 TTCCGGGGCC ACTTCCTGAT CGAGGGCGAC CTGAACCCCG ACAACAGCGA CGTGGACAAG 1921 CTGTTCATCC AGCTGGTGCA GACCTACAAC CAGCTGTTCG AGGAAAACCC CATCAACGCC 1981 AGCGGCGTGG ACGCCAAGGC CATCCTGTCT GCCAGACTGA GCAAGAGCAG ACGGCTGGAA 2041 AATCTGATCG CCCAGCTGCC CGGCGAGAAG AAGAATGGCC TGTTCGGAAA CCTGATTGCC 2101 CTGAGCCTGG GCCTGACCCC CAACTTCAAG AGCAACTTCG ACCTGGCCGA GGATGCCAAA 2161 CTGCAGCTGA GCAAGGACAC CTACGACGAC GACCTGGACA ACCTGCTGGC CCAGATCGGC 2221 GACCAGTACG CCGACCTGTT TCTGGCCGCC AAGAACCTGT CCGACGCCAT CCTGCTGAGC 2281 GACATCCTGA GAGTGAACAC CGAGATCACC AAGGCCCCCC TGAGCGCCTC TATGATCAAG 2341 AGATACGACG AGCACCACCA GGACCTGACC CTGCTGAAAG CTCTCGTGCG GCAGCAGCTG 2401 CCTGAGAAGT ACAAAGAGAT TTTCTTCGAC CAGAGCAAGA ACGGCTACGC CGGCTACATT 2461 GACGGCGGAG CCAGCCAGGA AGAGTTCTAC AAGTTCATCA AGCCCATCCT GGAAAAGATG 2521 GACGGCACCG AGGAACTGCT CGTGAAGCTG AACAGAGAGG ACCTGCTGCG GAAGCAGCGG 2581 ACCTTCGACA ACGGCAGCAT CCCCCACCAG ATCCACCTGG GAGAGCTGCA CGCCATTCTG 2641 CGGCGGCAGG AAGATTTTTA CCCATTCCTG AAGGACAACC GGGAAAAGAT CGAGAAGATC 2701 CTGACCTTCC GCATCCCCTA CTACGTGGGC CCTCTGGCCA GGGGAAACAG CAGATTCGCC 2761 TGGATGACCA GAAAGAGCGA GGAAACCATC ACCCCCTGGA ACTTCGAGGA AGTGGTGGAC 2821 AAGGGCGCTT CCGCCCAGAG CTTCATCGAG CGGATGACCA ACTTCGATAA GAACCTGCCC 2881 AACGAGAAGG TGCTGCCCAA GCACAGCCTG CTGTACGAGT ACTTCACCGT GTATAACGAG 2941 CTGACCAAAG TGAAATACGT GACCGAGGGA ATGAGAAAGC CCGCCTTCCT GAGCGGCGAG 3001 CAGAAAAAGG CCATCGTGGA CCTGCTGTTC AAGACCAACC GGAAAGTGAC CGTGAAGCAG 3061 CTGAAAGAGG ACTACTTCAA GAAAATCGAG TGCTTCGACT CCGTGGAAAT CTCCGGCGTG 3121 GAAGATCGGT TCAACGCCTC CCTGGGCACA TACCACGATC TGCTGAAAAT TATCAAGGAC 3181 AAGGACTTCC TGGACAATGA GGAAAACGAG GACATTCTGG AAGATATCGT GCTGACCCTG 3241 ACACTGTTTG AGGACAGAGA GATGATCGAG GAACGGCTGA AAACCTATGC CCACCTGTTC 3301 GACGACAAAG TGATGAAGCA GCTGAAGCGG CGGAGATACA CCGGCTGGGG CAGGCTGAGC 3361 CGGAAGCTGA TCAACGGCAT CCGGGACAAG CAGTCCGGCA AGACAATCCT GGATTTCCTG 3421 AAGTCCGACG GCTTCGCCAA CAGAAACTTC ATGCAGCTGA TCCACGACGA CAGCCTGACC 3481 TTTAAAGAGG ACATCCAGAA AGCCCAGGTG TCCGGCCAGG GCGATAGCCT GCACGAGCAC 3541 ATTGCCAATC TGGCCGGCAG CCCCGCCATT AAGAAGGGCA TCCTGCAGAC AGTGAAGGTG 3601 GTGGACGAGC TCGTGAAAGT GATGGGCCGG CACAAGCCCG AGAACATCGT GATCGAAATG 3661 GCCAGAGAGA ACCAGACCAC CCAGAAGGGA CAGAAGAACA GCCGCGAGAG AATGAAGCGG 3721 ATCGAAGAGG GCATCAAAGA GCTGGGCAGC CAGATCCTGA AAGAACACCC CGTGGAAAAC 3781 ACCCAGCTGC AGAACGAGAA GCTGTACCTG TACTACCTGC AGAATGGGCG GGATATGTAC 3841 GTGGACCAGG AACTGGACAT CAACCGGCTG TCCGACTACG ATGTGGACCA TATCGTGCCT 3901 CAGAGCTTTC TGAAGGACGA CTCCATCGAC AACAAGGTGC TGACCAGAAG CGACAAGAAC 3961 CGGGGCAAGA GCGACAACGT GCCCTCCGAA GAGGTCGTGA AGAAGATGAA GAACTACTGG 4021 CGGCAGCTGC TGAACGCCAA GCTGATTACC CAGAGAAAGT TCGACAATCT GACCAAGGCC 4081 GAGAGAGGCG GCCTGAGCGA ACTGGATAAG GCCGGCTTCA TCAAGAGACA GCTGGTGGAA 4141 ACCCGGCAGA TCACAAAGCA CGTGGCACAG ATCCTGGACT CCCGGATGAA CACTAAGTAC 4201 GACGAGAATG ACAAGCTGAT CCGGGAAGTG AAAGTGATCA CCCTGAAGTC CAAGCTGGTG 4261 TCCGATTTCC GGAAGGATTT CCAGTTTTAC AAAGTGCGCG AGATCAACAA CTACCACCAC 4321 GCCCACGACG CCTACCTGAA CGCCGTCGTG GGAACCGCCC TGATCAAAAA GTACCCTAAG 4381 CTGGAAAGCG AGTTCGTGTA CGGCGACTAC AAGGTGTACG ACGTGCGGAA GATGATCGCC 4441 AAGAGCGAGC AGGAAATCGG CAAGGCTACC GCCAAGTACT TCTTCTACAG CAACATCATG 4501 AACTTTTTCA AGACCGAGAT TACCCTGGCC AACGGCGAGA TCCGGAAGCG GCCTCTGATC 4561 GAGACAAACG GCGAAACCGG GGAGATCGTG TGGGATAAGG GCCGGGATTT TGCCACCGTG 4621 CGGAAAGTGC TGAGCATGCC CCAAGTGAAT ATCGTGAAAA AGACCGAGGT GCAGACAGGC 4681 GGCTTCAGCA AAGAGTCTAT CCTGCCCAAG AGGAACAGCG ATAAGCTGAT CGCCAGAAAG 4741 AAGGACTGGG ACCCTAAGAA GTACGGCGGC TTCGACAGCC CCACCGTGGC CTATTCTGTG 4801 CTGGTGGTGG CCAAAGTGGA AAAGGGCAAG TCCAAGAAAC TGAAGAGTGT GAAAGAGCTG 4861 CTGGGGATCA CCATCATGGA AAGAAGCAGC TTCGAGAAGA ATCCCATCGA CTTTCTGGAA 4921 GCCAAGGGCT ACAAAGAAGT GAAAAAGGAC CTGATCATCA AGCTGCCTAA GTACTCCCTG 4981 TTCGAGCTGG AAAACGGCCG GAAGAGAATG CTGGCCTCTG CCGGCGAACT GCAGAAGGGA 5041 AACGAACTGG CCCTGCCCTC CAAATATGTG AACTTCCTGT ACCTGGCCAG CCACTATGAG 5101 AAGCTGAAGG GCTCCCCCGA GGATAATGAG CAGAAACAGC TGTTTGTGGA ACAGCACAAG 5161 CACTACCTGG ACGAGATCAT CGAGCAGATC AGCGAGTTCT CCAAGAGAGT GATCCTGGCC 5221 GACGCTAATC TGGACAAAGT GCTGTCCGCC TACAACAAGC ACCGGGATAA GCCCATCAGA 5281 GAGCAGGCCG AGAATATCAT CCACCTGTTT ACCCTGACCA ATCTGGGAGC CCCTGCCGCC 5341 TTCAAGTACT TTGACACCAC CATCGACCGG AAGAGGTACA CCAGCACCAA AGAGGTGCTG 5401 GACGCCACCC TGATCCACCA GAGCATCACC GGCCTGTACG AGACACGGAT CGACCTGTCT 5461 CAGCTGGGAG GCGACAAAAG GCCGGCGGCC ACGAAAAAGG CCGGCCAGGC AAAAAAGAAA 5521 AAGgaattcG GCAGTGGAGA GGGCAGAGGA AGTCTGCTAA CATGCGGTGA CGTCGAGGAG 5581 AATCCTGGCC CAATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC 5641 CCCAGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC 5701 GTCGATCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC 5761 GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG 5821 ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC 5881 GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC 5941 CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGAG TCTCGCCCGA CCACCAGGGC 6001 AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG 6061 CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC 6121 ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG 6181 CCCGGTGCCT GAgaattcta aCTAGAGCTC GCTGATCAGC CTCGACTGTG CCTTCTAGTT 6241 GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC 6301 CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT 6361 CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GAgAATAGCA 6421 GGCATGCTGG GGAgcggccg caggaacccc tagtgatgga gttggccact ccctctctgc 6481 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc 6541 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt 6601 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc 6661 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 6721 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 6781 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 6841 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc 6901 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 6961 cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg 7021 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 7081 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc 7141 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg 7201 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat 7261 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg 7321 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt 7381 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 7441 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 7501 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 7561 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 7621 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 7681 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 7741 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 7801 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 7861 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 7921 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 7981 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 8041 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 8101 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 8161 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtggaagccg 8221 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 8281 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 8341 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 8401 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 8461 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 8521 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 8581 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 8641 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 8701 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 8761 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 8821 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 8881 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 8941 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 9001 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 9061 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 9121 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.