pCMV-CHRND-P2A-CHRNG-T2A-mCher

pCMV-CHRND-P2A-CHRNG-T2A-mCherry-Neo plasmid

PVT90632

pCMV-CHRND-P2A-CHRNG-T2A-mCherry-Neo plasmid Information

Plasmid name: pCMV-CHRND(human)-P2A-CHRNG(human)-T2A-mCherry-Neo
Alias: NM_000751.3；ACHRD; CMS2A; CMS3A; CMS3B; CMS3C; FCCMS;SCCMS/NM_005199.5；ACHRG
Gene lenght: 1551，1551bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer:
Reverse primer: BGH-R
Copy number: Low
Induction:

pCMV-CHRND-P2A-CHRNG-T2A-mCherry-Neo plasmid Description

pCMV-CHRND-P2A-CHRNG-T2A-mCherry-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-CHRND-P2A-CHRNG-T2A-mCherry-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CACTAGTCCA GTGTGGTGGA ATTCGCCACC atggaggggc cagtgctgac actggggctg 961 ctggctgccc tggcggtgtg tggcagctgg gggctgaacg aggaggagcg gctgatccgg 1021 cacctgtttc aagagaaggg ctacaacaag gagctccggc ccgtggcaca caaagaggag 1081 agtgtggacg ttgccctggc cctcacactc tccaacctca tctccctgaa agaagttgag 1141 gagaccctca ctaccaatgt gtggatagag cacggctgga cagacaaccg gctgaagtgg 1201 aatgctgaag aatttggaaa catcagtgtc ctgcgcctcc ccccggacat ggtgtggctc 1261 ccagagattg tgctggagaa caacaatgac ggctccttcc agatctccta ctcctgcaac 1321 gtgcttgtct accactacgg cttcgtgtac tggctgccac ctgccatctt ccgctcctcc 1381 tgccccatct ctgtcaccta tttccccttc gactggcaga actgctccct caagttcagt 1441 tccctcaagt atacggccaa agagatcacc ctgagcctga aacaggatgc caaggagaac 1501 cgcacctacc ccgtggagtg gatcatcatt gatcctgaag gcttcacaga gaacggggag 1561 tgggagatag tccaccggcc ggccagggtc aacgtggacc ccagagcccc tctggacagc 1621 cccagccgcc aggacatcac cttctacctc atcatccgcc gcaagcccct cttctacatc 1681 atcaacatcc tggtgccctg cgtgctcatc tccttcatgg tcaacctggt cttctaccta 1741 ccggctgaca gtggtgagaa gacatcagtg gccatctcgg tgctcctggc tcagtctgtc 1801 ttcctgctgc tcatctccaa gcgtctgcct gccacatcca tggccatccc ccttatcggc 1861 aagttcctgc tcttcggcat ggtgctggtc accatggttg tggtgatctg tgtcatcgtg 1921 ctcaacatcc acttccgaac acccagcacc catgtgctgt ctgagggggt caagaagctc 1981 ttcctggaga ccctgccgga gctcctgcac atgtcccgcc cagcagagga tggacccagc 2041 cctggggccc tggtgcggag gagcagctcc ctgggataca tctccaaggc cgaggagtac 2101 ttcctgctca agtcccgcag tgacctcatg ttcgagaagc agtcagagcg gcatgggctg 2161 gccaggcgcc tcaccactgc acgccggccc ccagcaagct ctgagcaggc ccagcaggaa 2221 ctcttcaatg agctgaagcc agctgtggat ggggcaaact tcattgttaa ccacatgagg 2281 gaccagaaca attacaatga ggagaaagac agctggaacc gagtggcccg cacagtggac 2341 cgcctctgcc tgtttgtggt gacgcctgtc atggtggtgg gcacagcctg gatcttcctg 2401 cagggcgttt acaaccagcc accaccccag ccttttcctg gggaccccta ctcctacaac 2461 gtgcaggaca agcgcttcat cggaagcgga gctactaact tcagcctgct gaagcaggct 2521 ggagacgtgg aggagaaccc tggacctatg catgggggcc aggggccgct gctcctcctg 2581 ctgctgctgg ctgtctgcct gggggcccag ggccggaacc aggaggagcg cctgctcgca 2641 gacctgatgc aaaactacga ccccaacctg cggcccgcgg aacgagactc ggatgtggtc 2701 aatgtcagcc tgaagctaac cctcaccaac ctcatctccc tgaacgagcg agaggaagcc 2761 ctcaccacca atgtctggat agagatgcag tggtgcgact atcgcctgcg ctgggatccg 2821 cgagactacg aaggcctgtg ggtgctgagg gtgccgtcca ccatggtgtg gcggccggat 2881 atcgtgctgg agaacaacgt ggacggtgtc ttcgaggtgg ccctctactg caatgtgctc 2941 gtgtcccctg acggctgtat ctactggctg ccgcctgcca tcttccgttc cgcctgctct 3001 atctcagtca cctacttccc cttcgactgg cagaactgct cccttatctt ccagtcccag 3061 acttacagca ccaatgagat tgatctgcag ctgagtcagg aagatggcca gaccatcgag 3121 tggattttca ttgaccctga ggccttcaca gagaatgggg agtgggccat ccagcaccga 3181 ccagccaaga tgctcctgga cccagcggcg ccagcccagg aagcaggcca ccagaaggtg 3241 gtgttctacc tgctcatcca gcgcaagccc ctcttctacg tcatcaacat catcgccccc 3301 tgtgtgctca tctcctctgt cgccatcctc atccacttcc ttcctgccaa ggctgggggc 3361 cagaagtgta ccgtcgccat caacgtgctc ctggcccaga ctgtcttcct cttccttgtg 3421 gccaagaagg tgcctgaaac ctcccaggcg gtgccactca tcagcaagta cctgaccttc 3481 ctcctggtgg tgaccatcct cattgtcgtg aatgctgtgg ttgtgctcaa tgtctccttg 3541 cggtctccac acacacactc catggcccga ggggtccgca aggtgttcct gaggctcttg 3601 ccccagctgc tgaggatgca cgttcgcccg ctggccccgg cagctgtgca ggacacccag 3661 tcccggctac agaatggctc ctcgggatgg tcgatcacaa ctggggagga ggtggccctc 3721 tgcctgcctc gcagtgaact cctcttccag cagtggcagc ggcaagggct ggtggcggca 3781 gcgctggaga agctagagaa aggcccggag ttagggctga gccagttctg tggcagcctg 3841 aagcaggctg ccccagccat ccaggcctgt gtggaagcct gcaacctcat tgcctgtgcc 3901 cggcaccagc agagtcactt tgacaatggg aatgaggagt ggttcctggt gggccgagtg 3961 ctggaccgcg tctgcttcct ggccatgctc tcgctcttca tctgtggcac agctggcatc 4021 ttcctcatgg cccactacaa ccgggtgccg gccctgccat tccctggaga tccacgcccc 4081 tacctgccct caccagacga gggcagagga agtcttctaa catgcggtga cgtggaggag 4141 aatcccggcc ctatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 4201 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 4261 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 4321 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 4381 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 4441 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 4501 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 4561 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 4621 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 4681 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 4741 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 4801 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 4861 TAGCTCGAGT CTAGAGGGCC CGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAGT 4921 TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT 4981 CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT 5041 TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC 5101 AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC 5161 TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 5221 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 5281 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 5341 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 5401 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 5461 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 5521 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 5581 ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA 5641 AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA 5701 CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 5761 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA 5821 GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG 5881 CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT 5941 TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT 6001 GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG 6061 TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG 6121 TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG 6181 CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC 6241 CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG 6301 AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA 6361 TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC 6421 AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG 6481 ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG 6541 CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA 6601 TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG 6661 ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT 6721 GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT 6781 TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA 6841 AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT 6901 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT 6961 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG 7021 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT 7081 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT 7141 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA 7201 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT 7261 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT 7321 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC 7381 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA 7441 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 7501 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 7561 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 7621 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 7681 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 7741 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 7801 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 7861 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 7921 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 7981 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 8041 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT 8101 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 8161 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 8221 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 8281 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT 8341 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC 8401 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG 8461 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG 8521 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT 8581 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT 8641 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT 8701 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT 8761 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT 8821 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT 8881 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC 8941 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA 9001 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA 9061 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA 9121 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT 9181 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 9241 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 9301 TGACGTCGAC GGATCGGGAG ATCTCCCGAT CCCCTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.