pLV3-CMV-FANCI-3×FLAG-CopGFP-P

pLV3-CMV-FANCI-3×FLAG-CopGFP-Puro plasmid

PVT90588

pLV3-CMV-FANCI-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-FANCI(human)-3×FLAG-CopGFP-Puro
Alias: NM_001113378.2;KIAA1794
Gene lenght: 3984bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-FANCI-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-FANCI-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-FANCI-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg accagaagat tttatctcta 2341 gcagcagaaa aaacagcaga caaactgcaa gaatttcttc aaaccctgag agaaggtgat 2401 ttgactaatc tccttcagaa tcaagcagtg aaaggaaaag ttgctggagc actcctgaga 2461 gccatcttca aaggttcccc ctgctctgag gaagctggaa cacttaggag acgtaagata 2521 tacacttgtt gtatccagtt ggtggaatcg ggggatttgc agaaagaaat agcgtctgag 2581 atcataggat tactgatgct ggaggctcac cattttccag gaccattatt ggttgaatta 2641 gccaatgagt ttattagtgc tgtcagagaa ggcagcctag tgaatggaaa atctttggag 2701 ttactaccta tcattctcac tgccctggct acgaaaaagg aaaatctggc ttatggaaaa 2761 ggtgtactga gtggggaaga atgtaagaaa cagttgatta acaccctgtg ttctggcagg 2821 tgggatcagc aatatgtaat ccaactcacc tccatgttca aggatgtccc tctgactgca 2881 gaagaggtgg aatttgtggt ggaaaaagca ttgagcatgt tctccaagat gaatcttcaa 2941 gaaataccac ctttggtcta tcagcttctg gttctctcct ccaagggaag cagaaagagt 3001 gttttggaag gaatcatagc cttcttcagt gcactagata agcagcacaa tgaggaacag 3061 agtggtgacg agctattgga tgttgtcact gtgccatcag gtgaacttcg tcatgtggaa 3121 ggcaccatta ttctacacat tgtgtttgcc atcaaattgg actatgaact aggcagagaa 3181 ctcgtgaaac acttaaaggt aggacagcaa ggagattcca ataataactt aagtcccttc 3241 agcattgctc ttcttctgtc tgtaacaaga atacaaagat ttcaggacca ggtgcttgat 3301 cttttaaaga cttcggttgt aaagagcttt aaggatcttc aactcctcca aggctcaaaa 3361 tttcttcaga atctagttcc tcatagatct tatgtttcaa ccatgatctt ggaagtagtg 3421 aagaatagcg ttcatagctg ggaccatgtt actcagggcc tcgtagaact tggtttcatt 3481 ttgatggatt catatgggcc aaagaaggtt cttgatggaa aaactattga aaccagccca 3541 agtctttcta gaatgccaaa ccagcatgca tgtaagctcg gagctaatat cctgttggaa 3601 acttttaaga tccatgagat gatcagacaa gaaattttgg agcaggtcct caacagggtt 3661 gttaccagag catcttctcc catcagtcat ttcttagacc tgctttcaaa tatcgtcatg 3721 tatgcaccct tagttcttca aagttgttct tctaaagtca cagaagcttt tgactatttg 3781 tcctttctgc cccttcagac tgtacaaagg ctgcttaagg cagtgcagcc ccttctcaaa 3841 gtcagcatgt caatgagaga ctgcttgata cttgtccttc ggaaagctat gtttgccaac 3901 cagcttgatg cccgaaaatc tgcagttgct gggtttttgc tgctcctgaa gaactttaaa 3961 gttttaggca gcctgtcatc ctctcagtgc agtcagtctc tcagtgtcag tcaggttcat 4021 gtggatgttc acagccatta caattctgtc gccaatgaaa ctttttgcct tgagatcatg 4081 gatagtttga ggagatgctt aagccagcaa gctgatgttc gactcatgct ttatgagggg 4141 ttttatgatg ttcttcgaag gaactctcag ctggctaatt cagtcatgca aactctgctc 4201 tcacagttaa aacagttcta tgagccaaaa cctgatctgc tgcctcctct gaaattagaa 4261 gcttgtattc tgacccaagg agataagatc tctctacaag aaccactgga ttatctgctg 4321 tgttgtattc agcattgttt ggcctggtat aagaatacag tcataccctt acagcaggga 4381 gaggaggaag aggaggagga agaggcattc tacgaagacc tagatgatat attggagtcc 4441 attactaata gaatgattaa gagtgagctg gaagactttg aactggataa atcagcagat 4501 ttttctcaga gcaccagtat tggcataaaa aataatatct gtgcttttct tgtgatggga 4561 gtttgtgagg ttttaataga atacaatttc tccataagta gtttcagtaa gaataggttt 4621 gaggacattc tgagcttatt tatgtgttac aaaaaactct ctgacattct taatgaaaaa 4681 gcgggtaaag ccaaaactaa aatggccaac aagacaagtg atagtctttt gtccatgaaa 4741 tttgtgtcca gtcttctcac tgctcttttc agggatagta tccaaagcca ccaagaaagc 4801 ctttctgttc tcaggtccag caatgagttt atgcgctatg cagtgaatgt agctctgcag 4861 aaagtacagc agctaaagga aacagggcat gtgagtggcc ctgatggcca aaacccagaa 4921 aagatctttc agaacctctg tgacataact cgagtcttgc tatggagata cacttcaatt 4981 cctacttcag tggaagagtc gggaaagaaa gagaaaggaa agagcatctc actgctgtgc 5041 ttggagggtt tacagaaaat attcagtgct gtgcaacagt tctatcagcc caagattcag 5101 cagtttctca gagctctgga tgtcacagat aaggaaggag aagagagaga agatgcagat 5161 gtcagtgtca ctcagagaac agcattccag atccggcaat ttcagaggtc cttgttgaat 5221 ttacttagca gtcaagagga agattttaat agcaaagaag ccctcctgct agtcacggtt 5281 cttaccagtt tgtccaagtt actggagccc tcctctcctc agtttgtgca gatgttatcc 5341 tggacatcaa agatttgcaa ggaaaacagc cgggaggatg ccttgttttg caagagcttg 5401 atgaacttgc tcttcagcct gcatgtttcg tataagagtc ctgtcattct gctgcgtgac 5461 ttgtcccagg atatccacgg gcatctggga gatatagacc aggatgtaga ggtggagaaa 5521 acaaaccact ttgcaatagt gaatttgaga acggctgccc ccactgtctg tttacttgtt 5581 ctgagtcagg ccgagaaggt tctagaagaa gtggactggc taatcaccaa gcttaaggga 5641 caagtgagcc aagaaacctt atcagaagag gcctcttctc aggcaaccct accaaatcag 5701 cctgttgaga aagctatcat catgcaactg ggaactctgc ttacattttt ccacgagctg 5761 gtgcagacag ctctgccatc aggcagctgt gtggacacct tgttaaagga cttgtgcaaa 5821 atgtacacca cacttacagc ccttgtcaga tattatctcc aggtgtgtca gagctccgga 5881 ggaattccaa aaaatatgga aaagctggtg aagctgtctg gttctcatct gacccccctg 5941 tgttattctt tcatttctta cgtacagaat aagagtaaga gcctgaacta tacgggagag 6001 aaaaaggaga aacctgctgc cgttgccaca gccatggcca gagttcttcg ggaaaccaag 6061 ccaatcccta acctcatctt tgccatagaa cagtatgaaa aatttctcat ccacctttct 6121 aagaagtcca aggtgaacct gatgcagcac atgaagctca gcacctcacg agacttcaag 6181 atcaaaggaa acatcctaga catggttctt cgagaggatg gtgaagatga aaatgaagag 6241 ggcactgcat cagagcatgg gggacagaac aaagaaccag ccaagaagaa aaggaaaaaa 6301 ggatccGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT 6361 GACGATGACA AGtagcggcc gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca 6421 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt 6481 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt 6541 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt 6601 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca 6661 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct 6721 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg 6781 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg 6841 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt 6901 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc 6961 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc 7021 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag 7081 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc 7141 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac 7201 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg 7261 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc 7321 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg 7381 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc 7441 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc 7501 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag 7561 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc 7621 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc 7681 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag 7741 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 7801 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 7861 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 7921 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 7981 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 8041 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 8101 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 8161 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 8221 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 8281 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 8341 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 8401 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 8461 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 8521 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 8581 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 8641 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 8701 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaATC ATCGTCCTTT 8761 CCTtggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 8821 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 8881 cttccgcgtc tTcgccttcg ccctcagacg agtcggatct ccctttggGc cgcctccccg 8941 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 9001 agaaaagggg ggactggaag ggctaattca ctcccaacga aGataagatc tgctttttgc 9061 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 9121 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 9181 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 9241 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 9301 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 9361 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 9421 caaactcatc aatgtatctt atcatgtctg gctctagcta TCCCGCCCCT AACTCCGCCC 9481 Atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 9541 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 9601 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt 9661 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 9721 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 9781 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 9841 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 9901 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 9961 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 10021 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 10081 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 10141 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 10201 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 10261 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 10321 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 10381 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 10441 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 10501 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 10561 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 10621 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 10681 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 10741 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 10801 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 10861 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 10921 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 10981 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 11041 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 11101 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 11161 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 11221 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 11281 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 11341 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 11401 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 11461 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 11521 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 11581 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 11641 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 11701 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 11761 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 11821 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 11881 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 11941 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 12001 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 12061 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 12121 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 12181 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 12241 acgacgttgt aaaacgacgg ccagtgccaa gctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.