pLV3-CMV-SPDEF-3×FLAG-CopGFP-P

pLV3-CMV-SPDEF-3×FLAG-CopGFP-Puro plasmid

PVT90583

pLV3-CMV-SPDEF-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SPDEF(human)-3×FLAG-CopGFP-Puro
Alias: NM_012391.3;bA375E1.3; PDEF
Gene lenght: 1005bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SPDEF-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-SPDEF-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SPDEF-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg gcagcgccag cccgggtctg 2341 agcagcgtat cccccagcca cctcctgctg ccccccgaca cggtgtcgcg gacaggcttg 2401 gagaaggcgg cagcgggggc agtgggtctc gagagacggg actggagtcc cagtccaccc 2461 gccacgcccg agcagggcct gtccgccttc tacctctcct actttgacat gctgtaccct 2521 gaggacagca gctgggcagc caaggcccct ggggccagca gtcgggagga gccacctgag 2581 gagcctgagc agtgcccggt cattgacagc caagccccag cgggcagcct ggacttggtg 2641 cccggcgggc tgaccttgga ggagcactcg ctggagcagg tgcagtccat ggtggtgggc 2701 gaagtgctca aggacatcga gacggcctgc aagctgctca acatcaccgc agatcccatg 2761 gactggagcc ccagcaatgt gcagaagtgg ctcctgtgga cagagcacca ataccggctg 2821 ccccccatgg gcaaggcctt ccaggagctg gcgggcaagg agctgtgcgc catgtcggag 2881 gagcagttcc gccagcgctc gcccctgggt ggggatgtgc tgcacgccca cctggacatc 2941 tggaagtcag cggcctggat gaaagagcgg acttcacctg gggcgattca ctactgtgcc 3001 tcgaccagtg aggagagctg gaccgacagc gaggtggact catcatgctc cgggcagccc 3061 atccacctgt ggcagttcct caaggagttg ctactcaagc cccacagcta tggccgcttc 3121 attaggtggc tcaacaagga gaagggcatc ttcaaaattg aggactcagc ccaggtggcc 3181 cggctgtggg gcatccgcaa gaaccgtccc gccatgaact acgacaagct gagccgctcc 3241 atccgccagt attacaagaa gggcatcatc cggaagccag acatctccca gcgcctcgtc 3301 taccagttcg tgcaccccat cggatccGAC TACAAAGACC ATGACGGTGA TTATAAAGAT 3361 CATGACATCG ACTACAAGGA TGACGATGAC AAGtagcggc cgcGaaggat ctgcgatcgc 3421 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 3481 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 3541 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 3601 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga 3661 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 3721 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 3781 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 3841 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 3901 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc 3961 accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc 4021 accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc 4081 cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg 4141 agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac 4201 cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag 4261 gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc 4321 gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc 4381 atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc 4441 agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc 4501 cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc 4561 gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag 4621 cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc 4681 gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta 4741 acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc 4801 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 4861 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 4921 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 4981 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 5041 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 5101 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 5161 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 5221 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 5281 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 5341 acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt 5401 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 5461 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 5521 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 5581 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 5641 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 5701 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 5761 tcggggaaAT CATCGTCCTT TCCTtggctg ctcgcctgtg ttgccacctg gattctgcgc 5821 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 5881 ctgctgccgg ctctgcggcc tcttccgcgt ctTcgccttc gccctcagac gagtcggatc 5941 tccctttggG ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 6001 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 6061 aaGataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 6121 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 6181 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 6241 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 6301 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 6361 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 6421 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 6481 aTCCCGCCCC TAACTCCGCC CAtcccgccc ctaactccgc ccagttccgc ccattctccg 6541 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 6601 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt 6661 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 6721 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 6781 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 6841 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 6901 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 6961 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 7021 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 7081 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 7141 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 7201 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 7261 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 7321 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 7381 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 7441 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 7501 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 7561 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 7621 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 7681 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 7741 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 7801 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 7861 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 7921 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 7981 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 8041 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 8101 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 8161 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 8221 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 8281 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 8341 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 8401 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 8461 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 8521 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 8581 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 8641 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 8701 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 8761 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 8821 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 8881 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 8941 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 9001 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 9061 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 9121 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 9181 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 9241 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.