pLV2-CMV-AGR2-3×FLAG-Puro plas

pLV2-CMV-AGR2-3×FLAG-Puro plasmid

PVT96164

pLV2-CMV-AGR2-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-CMV-AGR2(human)-3×FLAG-Puro
Alias: NM_006408.4;AG-2; AG2; GOB-4; HAG-2; HEL-S-116; HPC8;PDIA17; RIFTD; XAG-2
Gene lenght: 525bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-AGR2-3×FLAG-Puro plasmid Description

pLV2-CMV-AGR2-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-AGR2-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatggagaa aattccagtg tcagcattct tgctccttgt 2881 ggccctctcc tacactctgg ccagagatac cacagtcaaa cctggagcca aaaaggacac 2941 aaaggactct cgacccaaac tgccccagac cctctccaga ggttggggtg accaactcat 3001 ctggactcag acatatgaag aagctctata taaatccaag acaagcaaca aacccttgat 3061 gattattcat cacttggatg agtgcccaca cagtcaagct ttaaagaaag tgtttgctga 3121 aaataaagaa atccagaaat tggcagagca gtttgtcctc ctcaatctgg tttatgaaac 3181 aactgacaaa cacctttctc ctgatggcca gtatgtcccc aggattatgt ttgttgaccc 3241 atctctgaca gttagagccg atatcactgg aagatattca aatcgtctct atgcttacga 3301 acctgcagat acagctctgt tgcttgacaa catgaagaaa gctctcaagt tgctgaagac 3361 tgaattggga tccGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA 3421 CAAGGATGAC GATGACAAGt agtctagata attctaccgg gtaggggagg cgcttttccc 3481 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg 3541 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg 3601 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca 3661 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat 3721 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct 3781 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag 3841 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac 3901 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac 3961 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 4021 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 4081 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 4141 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 4201 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 4261 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 4321 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 4381 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 4441 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 4501 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 4561 cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca aaatttgtga 4621 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 4681 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 4741 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 4801 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 4861 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 4921 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 4981 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg 5041 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 5101 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc 5161 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga 5221 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa 5281 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac 5341 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt 5401 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc 5461 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg 5521 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac 5581 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta 5641 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag 5701 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca 5761 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca 5821 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 5881 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 5941 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 6001 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 6061 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga 6121 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6181 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 6241 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6301 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6361 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6421 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6481 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6541 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6601 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6661 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6721 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6781 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6841 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 6901 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 6961 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7021 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7081 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7141 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 7201 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7261 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7321 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7381 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7441 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7501 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7561 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7621 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7681 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7741 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7801 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7861 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 7921 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 7981 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8041 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8101 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8161 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8221 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8281 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct 8341 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 8401 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac 8461 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact 8521 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt 8581 taaagaaatt gtatttgtta aatatgtact acaaacttag tagtttttaa agaaattgta 8641 tttgttaaat atgtactaca aacttagtag t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.