pCMV-USO1-3×FLAG-Neo plasmid

pCMV-USO1-3×FLAG-Neo plasmid

PVT90514

pCMV-USO1-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-USO1(human)-3×FLAG-Neo
Alias: NM_003715.4;P115; TAP; VDP
Gene lenght: 2886bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: T7
Reverse primer:
Copy number: High
Induction:

pCMV-USO1-3×FLAG-Neo plasmid Description

pCMV-USO1-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-USO1-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgaa tttcctccgc ggggtaatgg 781 ggggtcagag tgccggaccc cagcacacag aagccgagac gattcaaaag ctttgtgaca 841 gagtagcttc atctacttta ttggatgatc gaagaaatgc tgttcgtgct ctcaaatcat 901 tatctaagaa ataccgcttg gaagtgggta tacaagctat ggaacatctt attcatgttt 961 tacaaacaga tcgttcagat tctgaaataa taggttatgc tttggacaca ctatataata 1021 taatatctaa tgaagaagaa gaagaagtag aagaaaattc cacaagacag agtgaagatt 1081 tgggaagcca atttacagaa attttcatta agcagcagga aaatgtcact cttctgttat 1141 ctttattgga ggagtttgat ttccatgtcc gctggcctgg tgtgaagctt cttacttctc 1201 ttttaaaaca actagggcct caggtgcaac aaattatttt agtcagtcct atgggtgttt 1261 caagattgat ggacttacta gcggattcca gggaagttat acgtaatgat ggcgtcttac 1321 tactgcaggc actaacaaga agcaatggtg caatccagaa aattgttgct tttgaaaatg 1381 ctttcgagag actactggac attatttcag aggaggggaa cagtgatgga ggtatagtag 1441 ttgaagattg tttgattttg ctccaaaact tattaaaaaa caacaactcc aatcaaaatt 1501 tttttaaaga aggctcatat attcaacgta tgaaaccttg gtttgaagtt ggagatgaaa 1561 attctggctg gtctgcacag aaagtgacca atctacatct aatgctacag cttgttcgtg 1621 tattggtatc tcccaccaac cctcctggtg ctaccagtag ctgccagaag gctatgttcc 1681 agtgtgggtt attgcagcag ctttgtacta tcctaatggc tactggggtt cctgctgata 1741 tcctgactga gaccataaat actgtatcag aagttattcg aggctgccaa gtaaaccaag 1801 actactttgc atctgtaaat gcaccttcaa acccaccaag accggcaatt gtagtacttc 1861 tcatgtccat ggttaatgaa aggcagccat ttgttttgcg ctgtgctgtt ctctattgtt 1921 tccagtgttt cttgtataaa aaccaaaaag gacaaggaga aatcgtgtca acacttttac 1981 cttctaccat tgatgcaaca ggtaattcag tttcagctgg ccagttatta tgtggaggtt 2041 tgttttctac tgattcactt tcaaactggt gtgctgctgt ggcccttgcc catgcgttgc 2101 aagaaaatgc cacccagaaa gaacagttgc tcagggttca acttgctaca agtattggca 2161 accctccagt ttctttactt caacagtgca ccaatattct ttcacaggga agcaaaatac 2221 aaacaagagt tggattatta atgttgcttt gtacctggct aagcaattgt cccattgcag 2281 taacgcattt tcttcacaat tcagccaatg ttccatttct tacaggacaa attgcagaaa 2341 atcttggaga agaagagcag ttggtccaag gcttatgtgc ccttttgttg ggcatttcga 2401 tttatttcaa tgataactca cttgagagct acatgaaaga gaagctaaaa caactgattg 2461 agaagaggat tggcaaagag aatttcatag agaaactagg atttattagc aaacatgagt 2521 tgtattccag agcatctcag aaaccccagc caaactttcc cagtccagaa tacatgatat 2581 ttgatcatga gtttacgaag ctggtaaaag aacttgaagg tgttataact aaggctattt 2641 ataagtccag tgaagaagat aaaaaagaag aagaggtgaa aaaaacatta gaacagcatg 2701 acaatattgt gactcactac aaaaatatga ttcgagagca ggatctccaa cttgaggaat 2761 taaggcagca ggtttctaca ttaaaatgtc aaaatgaaca gctccagacg gcagtcacac 2821 agcaagtatc acagatccag cagcacaaag accagtataa tcttcttaaa atacagctag 2881 gaaaagacaa tcagcatcaa ggttcttaca gtgagggggc tcagatgaat ggcattcagc 2941 cagaagaaat tggtagattg cgagaagaga tagaagaatt aaaacgtaat caggaacttt 3001 tacaaagcca gctgactgaa aaggactcta tgattgaaaa tatgaaatct tcccaaacat 3061 ctggcacaaa tgaacagtct tcagcaatag tttcagctag agattctgaa caagttgcag 3121 aattaaaaca ggaactggca actttaaagt ctcagttaaa ctcacaatct gtggagatca 3181 ccaaactaca gacagaaaag caggaactgt tacagaaaac agaagcgttt gcaaaatcag 3241 ttgaggtaca aggagagacc gagactataa tagccaccaa aactactgat gtagaaggaa 3301 gactgtcagc attattacaa gagaccaaag agttaaagaa tgaaattaaa gctctgtctg 3361 aggaaagaac tgccattaaa gagcagctgg attcatctaa tagtaccatt gccattttac 3421 aaactgagaa agacaaacta gagttggaaa ttacagattc taaaaaagaa caagatgatc 3481 tcttggtgct cttggccgat caagatcaga aaatactgtc attgaagaat aaactcaagg 3541 atcttggtca tccagttgaa gaagaggatg aacttgaatc tggagaccaa gaggatgagg 3601 atgatgaaag tgaagatcct ggcaaggatc tagatcatat cGGATCCGAC TACAAAGACC 3661 ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGTAGTGAG 3721 GGCCCGATAT CTCGACAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT 3781 TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA 3841 TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC 3901 TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC 3961 TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT 4021 CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG 4081 GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC 4141 CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA 4201 CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG 4261 GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC 4321 CCCGCCTGGA AACGGGGGAG GCTAACTGAA ACACGGAAGG AGACAATACC GGAAGGAACC 4381 CGCGCTATGA CGGCAATAAA AAGACAGAAT AAAACGCACG GGTGTTGGGT CGTTTGTTCA 4441 TAAACGCGGG GTTCGGTCCC AGGGCTGGCA CTCTGTCGAT ACCCCACCGA GACCCCATTG 4501 GGGCCAATAC GCCCGCGTTT CTTCCTTTTC CCCACCCCAC CCCCCAAGTT CGGGTGAAGG 4561 CCCAGGGCTC GCAGCCAACG TCGGGGCGGC AGGCCCTGCC ATAGCAGATC TGCGCAGCTG 4621 GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT 4681 GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT 4741 CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGCAT 4801 CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG 4861 TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA 4921 GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC 4981 GGTCTATTCT TTTGATTTAT AAGGGATTTT GGGGATTTCG GCCTATTGGT TAAAAAATGA 5041 GCTGATTTAA CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT 5101 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 5161 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 5221 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 5281 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 5341 GAGGCCGCCT CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 5401 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 5461 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 5521 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 5581 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 5641 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 5701 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 5761 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 5821 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 5881 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 5941 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 6001 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 6061 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 6121 GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 6181 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 6241 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GCGAAATGAC 6301 CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA 6361 AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA 6421 TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA 6481 ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG 6541 TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA 6601 GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT 6661 TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG 6721 CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG 6781 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC 6841 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC 6901 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 6961 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 7021 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 7081 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 7141 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 7201 CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 7261 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 7321 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 7381 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 7441 TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 7501 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 7561 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 7621 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 7681 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 7741 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA 7801 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT 7861 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG 7921 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA 7981 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA 8041 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG 8101 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC 8161 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC 8221 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA 8281 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC 8341 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG 8401 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC 8461 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG 8521 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC 8581 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT 8641 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 8701 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 8761 AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGTC GACTCTCAGT 8821 ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATCTGC TCCCTGCTTG TGTGTTGGAG 8881 GTCGCTGAGT AGTGCGCGAG CAAAATTTAA GCTACAACAA GGCAAGGCTT GACCGACAAT 8941 TGCATGAAGA ATCTGCTTAG G

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.