pLV3-CMV-Kifc1-3×FLAG-CopGFP-P

pLV3-CMV-Kifc1-3×FLAG-CopGFP-Puro plasmid

PVT90491

pLV3-CMV-Kifc1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Kifc1(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_001195298.1;100042970; Gm4137; HSET; Kifc5a; KNSL2;Knsl2a; Tctex-7; Tctex-7A; Tctex7; Tctex7a
Gene lenght: 2022bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Kifc1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Kifc1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Kifc1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg acgtgcaggc gcagaggcca 2341 cctttgttgg aagtgaagag gaacgtagaa ctgaaggcag ccctggtgaa gtcctcctcc 2401 cgagtgcccc tgtcagcaag caggctcaag aggggtcctg accagatgga ggatgccttg 2461 gagcctgcaa agaaacggac acgagtcatg ggtgcagtga ccaaagttga cacatcccgt 2521 cccagaggac cactcctcag cacagtgtca cagacccagg gccacactgc agctcagaaa 2581 ggccctaaga agacaggacc tcgtgggtgc tctgctattg gtacagtgct gaggagccag 2641 aagccagttc ccgctgctcc tgcccagaag cctggcacat ccactgctcc tgtggtggta 2701 gggaagagag ctggcaaacg ccctgcctgg gacctgaagg gccagttgtg tgacctcaat 2761 gaagagttga aacgctatcg ggagaagact caaacgctgg aactggagaa ccggggtctt 2821 cgggagcaac tcagagaggt ccaggagcag gccacgaccc tggggacaga gcggaacacc 2881 ctggaagggg agctggccag tgtacgcagc cgagctgagc aggaccagca gaggctggag 2941 acgctgagtg cccgtgtctt ggagctggag gaatgtctgg gtaccaggga aaggctgctt 3001 caggagcttc agggagagcg gctgcaattg caggaggagc ggagcacact gagcacccag 3061 ctggaggagc aggagaggag gtttcaggcc acagaagcag ctctgtcaag cagccaagaa 3121 gaggtggtgt gtcttcggca gaagactgaa gcccaggtga ccttactggc tgagcaagga 3181 gaccggctct atgggttaga gatggagcgg cgacgactcc acaaccagct gcaggaactg 3241 aagggcaata tccgggtgtt ctgccgcgtg cgccctgtcc tcgaagggga atccactcca 3301 tctcctggct tcctcgtgtt tcctcctggc cctgctggac cctctgatcc cccgacgggc 3361 cttagcctct cacgatctga tgatcggcgc tccaccctga ctggggcccc ggcccccact 3421 gtccgccatg atttctcctt tgatcgggtg ttcccgccgg gaagcaagca ggaggaagtg 3481 tttgaggaga tcgccatgct tgtccagtca gcactggatg gctaccctgt gtgcattttt 3541 gcctatggac agacaggcag tggcaagacc ttcactatgg aaggagggcc taggggagac 3601 ccccaattgg aagggctgat ccctcgggcc atgcggcatc tgttctctgt ggcccaggag 3661 atgagcggcc agggctggac atacagtttt gtggcgagtt acgtagagat ctacaatgag 3721 accgttcgag acctgctagc tactgggccc cgcaagggac aagggggcga gtgtgagatc 3781 cgtcgggcaa gcccaggaag tgaggagctt actgtcacca atgcccgcta tgtccctgtt 3841 tcctgtgaga aagaggtgga ggccctgctc catttggctc atcagaaccg ggctgtggcc 3901 cacactgccc aaaataagag atcatcacgc agtcatagtg tgttccagct gcagatttct 3961 ggagagcatg cagctcgggg cctgcagtgt ggcgctccgc tcaaccttgt ggacctagct 4021 gggagtgagc ggctagaccc tggcttacac ctaggccctg gggagcgtga tcgtcttcgg 4081 gagacacagg ccattaacag cagtctgtct acactgggac tggtcataat ggccctgagc 4141 aataaggagt cccacgtgcc ttaccgaaac agcaagctca cctacttgct gcagaactct 4201 ctgggtggca gtgccaagat gcttatgttt gtgaatattt ctcctctgga agagaatgtc 4261 tccgagtctc tgaattcact acgctttgct tccaaggtga accagtgtgt cattggtact 4321 gctcaggcta ataagaaggg atccGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT 4381 GACATCGACT ACAAGGATGA CGATGACAAG tagcggccgc Gaaggatctg cgatcgctcc 4441 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 4501 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 4561 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 4621 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 4681 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 4741 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 4801 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 4861 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 4921 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 4981 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 5041 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 5101 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 5161 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 5221 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 5281 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 5341 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 5401 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 5461 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 5521 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 5581 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 5641 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 5701 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 5761 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc 5821 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 5881 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 5941 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 6001 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 6061 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 6121 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 6181 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 6241 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 6301 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 6361 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa 6421 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 6481 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 6541 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 6601 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 6661 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 6721 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 6781 gggaaATCAT CGTCCTTTCC Ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 6841 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 6901 ctgccggctc tgcggcctct tccgcgtctT cgccttcgcc ctcagacgag tcggatctcc 6961 ctttggGccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 7021 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaG 7081 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 7141 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 7201 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 7261 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 7321 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 7381 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 7441 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctaTC 7501 CCGCCCCTAA CTCCGCCCAt cccgccccta actccgccca gttccgccca ttctccgccc 7561 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 7621 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt 7681 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 7741 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 7801 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 7861 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 7921 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 7981 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 8041 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 8101 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 8161 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 8221 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 8281 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 8341 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 8401 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 8461 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 8521 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 8581 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 8641 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 8701 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 8761 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 8821 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 8881 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 8941 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 9001 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 9061 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 9121 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 9181 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 9241 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 9301 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 9361 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 9421 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 9481 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 9541 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 9601 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 9661 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 9721 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 9781 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 9841 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 9901 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 9961 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 10021 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 10081 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 10141 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 10201 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 10261 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.