pMSCV-Klf2-IRES-mCherry-Puro p

pMSCV-Klf2-IRES-mCherry-Puro plasmid

PVT90325

pMSCV-Klf2-IRES-mCherry-Puro plasmid Information

Plasmid name: pMSCV-Klf2(mouse)-IRES-mCherry-Puro
Alias: NM_008452.2;Lklf
Gene lenght: 1065bp
Host: Mammalian cells,Retrovirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: Non
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: PLXSN-F
Reverse primer:
Copy number: High
Induction:

pMSCV-Klf2-IRES-mCherry-Puro plasmid Description

pMSCV-Klf2-IRES-mCherry-Puro This is a plasmid suitable for protein expression in mammalian cells and can also be used for retrovirus packaging.

pMSCV-Klf2-IRES-mCherry-Puro plasmid Sequence

1 TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT 61 GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG AGAGACAGCA 121 GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA 181 ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA TCAGATGTTT 241 CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC CAATCAGTTC 301 GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG CCCACAACCC 361 CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT ATTCCCAATA 421 AAGCCTCTTG CTGTTTGCAT CCGAATCGTG GACTCGCTGA TCCTTGGGAG GGTCTCCTCA 481 GATTGATTGA CTGCCCACCT CGGGGGTCTT TCATTTGGAG GTTCCACCGA GATTTGGAGA 541 CCCCTGCCCA GGGACCACCG ACCCCCCCGC CGGGAGGTAA GCTGGCCAGC GGTCGTTTCG 601 TGTCTGTCTC TGTCTTTGTG CGTGTTTGTG CCGGCATCTA ATGTTTGCGC CTGCGTCTGT 661 ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC GAGTTCTGAA 721 CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTTGG GGGCCGTTTT TGTGGCCCGA 781 CCTGAGGAAG GGAGTCGATG TGGAATCCGA CCCCGTCAGG ATATGTGGTT CTGGTAGGAG 841 ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGTT TGGAACCGAA 901 GCCGCGCGTC TTGTCTGCTG CAGCGCTGCA GCATCGTTCT GTGTTGTCTC TGTCTGACTG 961 TGTTTCTGTA TTTGTCTGAA AATTAGGGCC AGACTGTTAC CACTCCCTTA AGTTTGACCT 1021 TAGGTCACTG GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT GTCAAGAAGA 1081 GACGTTGGGT TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA TGGCCGCGAG 1141 ACGGCACCTT TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT TCACCTGGCC 1201 CGCATGGACA CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG GCTTTTGACC 1261 CCCCTCCCTG GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT CCTCCATCCG 1321 CCCCGTCTCT CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC CTTTATCCAG 1381 CCCTCACTCC TTCTCTAGGC GCCGGAATTA GATCTCTCGA GGCCACCatg gcgctcagcg 1441 agcctatctt gccgtccttt gccactttcg ccagcccgtg cgagcgcggc ctccaggagc 1501 gctggccgcg aaatgaaccc gaggcgggcg gcacggatga ggacctaaac aacgtgttgg 1561 acttcatcct ctccatggga ttggacggtc tgggcgccga aaatcctccc gagcccccgc 1621 cgcagccccc gccgcctgcc ttctactacc cggagccggg tgctccgccg ccctacagca 1681 tccccgcggc cagcctggga acagagctgc tgcgccccga cctggacccg cctcaggggc 1741 cggctctgca cggccgcttc ctcctcgcgc ctcccgggcg gctagtgaag gccgagcccc 1801 ccgaggtgga cggcggcggc tacggctgcg ctccgggcct ggcccacgga ccgcgcgggc 1861 tgaagctcga gggcgcccca ggagcgacag gtgcatgcat gcggggtccc gccggccgcc 1921 ccccgccgcc cccggacacg ccgccgctca gccccgacgg ccccctgcgc atcccggcgt 1981 ccggtccccg caacccgttc ccgccgccct tcggtcccgg ccccagcttc ggcggtcccg 2041 gccccgcgtt gcactacggg cctcccgcgc ctggcgcctt cggtcttttc gaggacgcgg 2101 cggcagcggc ggcggcgctg ggcttggctc cacctgccac gcgcggtctc ctcacgccgc 2161 cctcgtcccc gctggagctg ctggaggcca agcccaaacg cggccgccgc tcctggcccc 2221 gcaagcgcgc cgccacacat acttgcagct acaccaactg cggcaagacc tacaccaaga 2281 gctcgcacct aaaggcgcat ctgcgtacac acacaggtga gaagccttat cattgcaact 2341 gggaaggatg cggctggaag ttcgcgcgct ctgacgagct tacccgccac taccgaaagc 2401 acacaggtca cagacccttt cagtgccact tgtgcgaccg ggccttctcc aggtccgacc 2461 acctggcctt gcacatgaag cgacacatgt agGAATTCTA CCGGGTAGGG GAGGCGCTTT 2521 TCCCAAGGCA GTCTGGAGCA TGCGCTTTAG CAGCCCCGCT GGGCACTTGG CGCTACACAA 2581 GTGGCCTCTG GCCTCGCACA CATTCCACAT CCACCGGTAG GCGCCAACCG GCTCCGTTCT 2641 TTGGTGGCCC CTTCGCGCCA CCTTCTACTC CTCCCCTAGT CAGGAAGTTC CCCCCCGCCC 2701 CGCAGCTCGC GTCGTGCAGG ACGTGACAAA TGGAAGTAGC ACGTCTCACT AGTCTCGTGC 2761 AGATGGACAG CACCGCTGAG CAATGGAAGC GGGTAGGCCT TTGGGGCAGC GGCCAATAGC 2821 AGCTTTGCTC CTTCGCTTTC TGGGCTCAGA GGCTGGGAAG GGGTGGGTCC GGGGGCGGGC 2881 TCAGGGGCGG GCTCAGGGGC GGGGCGGGCG CCCGAAGGTC CTCCGGAGGC CCGGCATTCT 2941 GCACGCTTCA AAAGCGCACG TCTGCCGCGC TGTTCTCCTC TTCCTCATCT CCGGGCCTTT 3001 CGACCTGCAG CCCAAGCTTA CCATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG 3061 CGACGACGTC CCCAGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC 3121 GCGCCACACC GTCGATCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT 3181 CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT 3241 GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC 3301 GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT 3361 GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA 3421 CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG 3481 CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG 3541 GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT 3601 GACCCGCAAG CCCGGTGCCT GACGCCCGCC CCACGACCCG CAGCGCCCGA CCGAAAGGAG 3661 CGCACGACCC CATGCATCCA ATTCCGCCCC CCCCCCCTAA CGTTACTGGC CGAAGCCGCT 3721 TGGAATAAGG CCGGTGTGCG TTTGTCTATA TGTTATTTTC CACCATATTG CCGTCTTTTG 3781 GCAATGTGAG GGCCCGGAAA CCTGGCCCTG TCTTCTTGAC GAGCATTCCT AGGGGTCTTT 3841 CCCCTCTCGC CAAAGGAATG CAAGGTCTGT TGAATGTCGT GAAGGAAGCA GTTCCTCTGG 3901 AAGCTTCTTG AAGACAAACA ACGTCTGTAG CGACCCTTTG CAGGCAGCGG AACCCCCCAC 3961 CTGGCGACAG GTGCCTCTGC GGCCAAAAGC CACGTGTATA AGATACACCT GCAAAGGCGG 4021 CACAACCCCA GTGCCACGTT GTGAGTTGGA TAGTTGTGGA AAGAGTCAAA TGGCTCTCCT 4081 CAAGCGTATT CAACAAGGGG CTGAAGGATG CCCAGAAGGT ACCCCATTGT ATGGGATCTG 4141 ATCTGGGGCC TCGGTGCACA TGCTTTACAT GTGTTTAGTC GAGGTTAAAA AAACGTCTAG 4201 GCCCCCCGAA CCACGGGGAC GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA 4261 ACCatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc 4321 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag 4381 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg 4441 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag 4501 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag 4561 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag 4621 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc 4681 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac 4741 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac 4801 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac 4861 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac 4921 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtagGTCGAC 4981 CTGCAGCCAA GCTTATCGAT AAAATAAAAG ATTTTATTTA GTCTCCAGAA AAAGGGGGGA 5041 ATGAAAGACC CCACCTGTAG GTTTGGCAAG CTAGCTTAAG TAACGCCATT TTGCAAGGCA 5101 TGGAAAATAC ATAACTGAGA ATAGAGAAGT TCAGATCAAG GTTAGGAACA GAGAGACAGC 5161 AGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT CAGGGCCAAG 5221 AACAGATGGT CCCCAGATGC GGTCCCGCCC TCAGCAGTTT CTAGAGAACC ATCAGATGTT 5281 TCCAGGGTGC CCCAAGGACC TGAAATGACC CTGTGCCTTA TTTGAACTAA CCAATCAGTT 5341 CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA GCCCACAACC 5401 CCTCACTCGG CGCGCCAGTC CTCCGATAGA CTGCGTCGCC CGGGTACCCG TGTATCCAAT 5461 AAACCCTCTT GCAGTTGCAT CCGACTTGTG GTCTCGCTGT TCCTTGGGAG GGTCTCCTCT 5521 GAGTGATTGA CTACCCGTCA GCGGGGGTCT TTCATGGGTA ACAGTTTCTT GAAGTTGGAG 5581 AACAACATTC TGAGGGTAGG AGTCGAATAT TAAGTAATCC TGACTCAATT AGCCACTGTT 5641 TTGAATCCAC ATACTCCAAT ACTCCTGAAA TAGTTCATTA TGGACAGCGC AGAAAGAGCT 5701 GGGGAGAATT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA 5761 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT 5821 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC 5881 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 5941 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 6001 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 6061 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 6121 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 6181 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 6241 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 6301 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 6361 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 6421 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 6481 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG 6541 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 6601 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA 6661 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 6721 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 6781 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 6841 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 6901 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 6961 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 7021 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 7081 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 7141 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 7201 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 7261 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 7321 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 7381 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 7441 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 7501 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 7561 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 7621 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 7681 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 7741 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 7801 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA 7861 TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC 7921 GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT 7981 GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG 8041 GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA 8101 TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCCATTCGC 8161 CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC 8221 AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC 8281 AGTCACGACG TTGTAAAACG ACGGCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA 8341 CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC 8401 GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC 8461 ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGCGAT TAGTCCAATT 8521 TGTTAAAGAC AGGATATCAG TGGTCCAGGC TCTAGTTTTG ACTCAACAAT ATCACCAGCT 8581 GAAGCCTATA GAGTACGAGC CATAGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA 8641 GGGGGGAA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.