pCMV-GRIA4-mCherry-Neo plasmid

pCMV-GRIA4-mCherry-Neo plasmid

PVT90100

pCMV-GRIA4-mCherry-Neo plasmid Information

Plasmid name: pCMV-GRIA4(human)-mCherry-Neo
Alias: NM_000829.4;GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;NEDSGA;
Gene lenght: 2706bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: mCherry-R
Copy number: High
Induction:

pCMV-GRIA4-mCherry-Neo plasmid Description

pCMV-GRIA4-mCherry-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-GRIA4-mCherry-Neo plasmid Sequence

1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 721 actctagagc tagcgtttaa acttaagctt ggtaccgagc tcggatccGC CACCatgagg 781 attatttcca gacagattgt cttgttattt tctggatttt ggggactcgc catgggagcc 841 tttccgagca gcgtgcaaat aggtggtctc ttcatccgaa acacagatca ggaatacact 901 gcttttcgat tagcaatttt tcttcataac accagcccca atgcgtcgga agctcctttt 961 aatttggtac ctcatgtgga caacattgag acagccaaca gttttgctgt aacaaacgcc 1021 ttctgttccc agtattctag aggagtattt gccatttttg gactctatga taagaggtcg 1081 gtacatacct tgacctcatt ctgcagcgcc ttacatatct ccctcatcac accaagtttc 1141 cctactgagg gggagagcca gtttgtgctg caactaagac cttcgttacg aggagcactc 1201 ttgagtttgc tggatcacta cgaatggaac tgttttgtct tcctgtatga cacagacagg 1261 ggatactcga tactccaagc tattatggaa aaagcaggac aaaatggttg gcatgtcagc 1321 gctatatgtg tggaaaattt taatgatgtc agctataggc aacttctaga agaacttgac 1381 agaagacaag agaagaagtt tgtaatagac tgtgagatag agagacttca aaacatatta 1441 gaacagattg taagtgttgg aaagcatgtt aaaggctacc attatatcat tgcaaacttg 1501 ggattcaagg atatttctct tgagaggttt atacatggtg gagccaatgt tactggattc 1561 cagttggtgg attttaatac acctatggta atcaaactaa tggatcgctg gaagaaacta 1621 gatcagagag agtatccagg atctgagact cctccaaagt acacctctgc tctgacttat 1681 gatggagtcc ttgtgatggc tgaaactttc cgaagtctta ggaggcagaa aattgatatc 1741 tcaaggagag gaaatgctgg ggattgtctg gcaaatcctg ctgctccatg gggccaggga 1801 attgacatgg agaggacact caaacaggtt cgaattcaag ggctgacagg gaatgttcag 1861 tttgaccact atggacgtag agtcaattac acaatggatg tgtttgagct gaaaagcaca 1921 ggacctagaa aggttggtta ctggaatgat atggataagt tagtcttgat tcaagatgta 1981 ccaactcttg gcaatgacac agctgctatt gagaacagaa cagtggttgt aaccacaatt 2041 atggaatccc catatgttat gtacaagaaa aatcatgaaa tgtttgaagg aaatgacaag 2101 tatgaaggat actgtgtaga tttggcatct gaaattgcaa aacatattgg tatcaagtat 2161 aaaattgcca ttgtccctga tggaaaatat ggagcaaggg atgcagacac aaaaatctgg 2221 aatgggatgg taggagaact tgtttatggg aaagcagaga ttgctattgc ccctctgaca 2281 atcactttgg tacgagagga ggtcattgac ttttctaagc ccttcatgag tttgggcata 2341 tctatcatga tcaaaaagcc tcagaaatcc aaaccaggag tgttttcctt cttggatcct 2401 ctggcctatg agatttggat gtgcatagtc tttgcctaca ttggtgtcag cgtggtctta 2461 ttcctagtta gtagatttag tccatatgag tggcacacag aagagccaga ggacggaaag 2521 gaaggaccca gcgaccagcc tcccaatgag tttggcatct ttaacagcct ctggttttcc 2581 ctgggtgctt ttatgcagca aggatgtgac atttcaccca gatccctctc aggtcgaatt 2641 gttggaggtg tttggtggtt ctttacactc atcattatat catcttatac tgctaacctc 2701 gctgctttcc tgacggttga gcgaatggtc tctcccatag aaagtgcaga agacctggcc 2761 aaacaaacag aaattgccta tggaacactg gattcaggat caacaaaaga attcttcaga 2821 agatcaaaaa tagcagtgta tgaaaagatg tggacctaca tgcgatcagc agagccatca 2881 gtattcacta ggactacagc tgagggagta gctcgtgtcc gcaaatccaa gggcaaattt 2941 gcctttctcc tggagtccac tatgaatgaa tacattgagc agcgaaagcc atgtgacacg 3001 atgaaagtgg gaggaaatct ggattccaaa ggctatggag tagcaacgcc caagggttcc 3061 tcattaagaa ctcctgtaaa ccttgccgtt ttgaaactca gtgaggcagg cgtcttagac 3121 aagctgaaaa acaaatggtg gtacgataaa ggtgaatgtg gacccaagga ctctggaagc 3181 aaggacaaga cgagtgcctt gagcctgagc aatgtagcag gcgtcttcta cattctggtt 3241 ggcggcttgg gcttggcaat gctggtggct ttgatagagt tctgttacaa gtccagggca 3301 gaagcgaaga gaatgaagct gaccttttct gaagccataa gaaacaaagc cagattatcc 3361 atcactggga gtgtgggaga gaatggccgc gtcttgacgc ctgactgccc aaaggctgta 3421 cacactggaa ctgcaatcag acaaagttca ggattggctg tcattgcatc ggacctacca 3481 gaattcggaa gcggagctac taacttcagc ctgctgaagc aggctggcga cgtggaggag 3541 aaccctggac ctatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 3601 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 3661 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 3721 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 3781 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 3841 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 3901 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 3961 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 4021 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 4081 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 4141 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 4201 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 4261 taactcgagt ctagagggcc ccttaagggt tcgatcccta ccggttagta atgagtttga 4321 tatctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 4381 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 4441 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 4501 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 4561 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 4621 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 4681 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca 4741 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 4801 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 4861 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct 4921 ggaaacgggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta 4981 tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc 5041 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa 5101 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg 5161 ctcgcagcca acgtcggggc ggcaggccct gccatagcag atctgcgcag ctggggctct 5221 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 5281 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 5341 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta 5401 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 5461 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 5521 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 5581 tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt 5641 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt 5701 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 5761 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 5821 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 5881 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 5941 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 6001 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag 6061 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 6121 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 6181 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 6241 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 6301 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 6361 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 6421 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 6481 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 6541 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc 6601 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 6661 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 6721 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 6781 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 6841 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa 6901 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg 6961 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg 7021 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc 7081 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 7141 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg 7201 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 7261 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 7321 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 7381 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 7441 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 7501 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 7561 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 7621 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 7681 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 7741 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 7801 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 7861 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 7921 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 7981 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 8041 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 8101 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 8161 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 8221 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 8281 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 8341 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 8401 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 8461 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 8521 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 8581 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 8641 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 8701 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 8761 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 8821 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 8881 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 8941 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 9001 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 9061 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 9121 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 9181 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 9241 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 9301 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 9361 cctgacgtcg acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct 9421 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg 9481 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga 9541 agaatctgct tagg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.