pAAV-U6-ENO2-shRNA3-ZsGreen1 p

pAAV-U6-ENO2-shRNA3-ZsGreen1 plasmid

PVT89793

pAAV-U6-ENO2-shRNA3-ZsGreen1 plasmid Information

Plasmid name: pAAV-U6-ENO2(human)-shRNA3-ZsGreen1
Alias: GGTCCAAGTTCACAGCCAATG;
Gene lenght:
Host: Mammalian cells,Adeno associated virus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pAAV-U6-ENO2-shRNA3-ZsGreen1 plasmid Description

pAAV-U6-ENO2-shRNA3-ZsGreen1 is a plasmid suitable for interference silencing in mammalian cells, and can also be used for adeno-associated virus packaging.

pAAV-U6-ENO2-shRNA3-ZsGreen1 plasmid Sequence

1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa 181 ttacggggtc attagttcat agcccatata tggagttccg CGTTACATAA CTTACGGTAA 241 ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG 301 TTCCCATAGT AACGcCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT 361 AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG 421 TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC 481 CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC 541 AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA 601 TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA 661 ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 721 GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC 781 TCCATAGAAG ACACCGGGAC CGATCCAGCC TCCGCGGATT CGAATCCCGG CCGGGAACGG 841 TGCATTGGAA CGCGGATTCC CCGTGCCAAG AGTGACGTAA GTACCGCCTA TAGAGTCTAT 901 AGGCCCACAA AAAATGCTTT CTTCTTTTAA TATACTTTTT TGTTTATCTT ATTTCTAATA 961 CTTTCCCTAA TCTCTTTCTT TCAGGGCAAT AATGATACAA TGTATCATGC CTCTTTGCAC 1021 CATTCTAAAG AATAACAGTG ATAATTTCTG GGTTAAGGCA ATAGCAATAT TTCTGCATAT 1081 AAATATTTCT GCATATAAAT TGTAACTGAT GTAAGAGGTT TCATATTGCT AATAGCAGCT 1141 ACAATCCAGC TACCATTCTG CTTTTATTTT ATGGTTGGGA TAAGGCTGGA TTATTCTGAG 1201 TCCAAGCTAG GCCCTTTTGC TAATCATGTT CATACCTCTT ATCTTCCTCC CACAGCTCCT 1261 GGGCAACGTG CTGGTCTGTG TGCTGGCCCA TCACTTTGGC AAAGAATTGG GATTCGAACA 1321 TCGATTGAAT TCCACCATGG CCCAGTCCAA GCACGGCCTG ACCAAGGAGA TGACCATGAA 1381 GTACCGCATG GAGGGCTGCG TGGACGGCCA CAAGTTCGTG ATCACCGGCG AGGGCATCGG 1441 CTACCCCTTC AAGGGCAAGC AGGCCATCAA CCTGTGCGTG GTGGAGGGCG GCCCCTTGCC 1501 CTTCGCCGAG GACATCTTGT CCGCCGCCTT CATGTACGGC AACCGCGTGT TCACCGAGTA 1561 CCCCCAGGAC ATCGTCGACT ACTTCAAGAA CTCCTGCCCC GCCGGCTACA CCTGGGACCG 1621 CTCCTTCCTG TTCGAGGACG GCGCCGTGTG CATCTGCAAC GCCGACATCA CCGTGAGCGT 1681 GGAGGAGAAC TGCATGTACC ACGAGTCCAA GTTCTACGGC GTGAACTTCC CCGCCGACGG 1741 CCCCGTGATG AAGAAGATGA CCGACAACTG GGAGCCCTCC TGCGAGAAGA TCATCCCCGT 1801 GCCCAAGCAG GGCATCTTGA AGGGCGACGT GAGCATGTAC CTGCTGCTGA AGGACGGTGG 1861 CCGCTTGCGC TGCCAGTTCG ACACCGTGTA CAAGGCCAAG TCCGTGCCCC GCAAGATGCC 1921 CGACTGGCAC TTCATCCAGC ACAAGCTGAC CCGCGAGGAC CGCAGCGACG CCAAGAACCA 1981 GAAGTGGCAC CTGACCGAGC ACGCCATCGC CTCCGGCTCC GCCTTGCCCT AAAGATCTAC 2041 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca 2101 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc 2161 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca 2221 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg 2281 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg 2341 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg 2401 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct 2461 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg 2521 attttgtagg taaccacgtg cggaccTCGA AATTCCCCAG TGGAAAGACG CGCAGGCAAA 2581 ACGCACCACG TGACGGAGCG TGACCGCGCG CCGAGCGCGC GCCAAGGTCG GGCAGGAAGA 2641 GGGCCTATTT CCCATGATTC CTTCATATTT GCATATACGA TACAAGGCTG TTAGAGAGAT 2701 AATTAGAATT AATTTGACTG TAAACACAAA GATATTAGTA CAAAATACGT GACGTAGAAA 2761 GTAATAATTT CTTGGGTAGT TTGCAGTTTT AAAATTATGT TTTAAAATGG ACTATCATAT 2821 GCTTACCGTA ACTTGAAAGT ATTTCGATTT CTTGGGTTTA TATATCTTGT GGAAAGGACG 2881 CGGGATCCGG GTCCAAGTTC ACAGCCAATG TTCAAGAGAC ATTGGCTGTG AACTTGGACC 2941 TTTTTTAAGC TTGCGGccgc aggaacccct agtgatggag ttggccactc cctctctgcg 3001 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg 3061 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt 3121 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg 3181 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 3241 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 3301 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 3361 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg 3421 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 3481 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg 3541 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 3601 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct 3661 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg 3721 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg 3781 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc 3841 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt 3901 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 3961 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 4021 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 4081 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 4141 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 4201 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 4261 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 4321 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 4381 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 4441 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 4501 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 4561 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 4621 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 4681 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 4741 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 4801 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 4861 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 4921 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 4981 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 5041 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 5101 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 5161 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 5221 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 5281 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 5341 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 5401 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 5461 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 5521 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 5581 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 5641 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.