pLV3-CMV-Senp1-3×FLAG-CopGFP-P

pLV3-CMV-Senp1-3×FLAG-CopGFP-Puro plasmid

PVT90312

pLV3-CMV-Senp1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Senp1(rat)-3×FLAG-CopGFP-Puro
Alias: NM_001432472.1;
Gene lenght: 2034bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Senp1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Senp1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Senp1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg aggacactgc cgacggggtg 2341 aagatggacg ctggggaggt gaccttagtg aaccacagct ccgccttcag agcccacctc 2401 ctgccacact caggctttcc agaggaccag cttgcacttt ctgaccagca gatcctgcct 2461 ttcaggcagg ggactctaca ccgagctttc acgggttcta caagcagtgc agcctataac 2521 ccagactatc actcagataa cccttcctca gacagtttcc ttggctcagg tgacataaga 2581 acctttggtc aaagtgcaaa tggacaatgg agaaactcca ctccagcatc aggatcagca 2641 ttacaaaaac caagaaatag ccgaagtctt tacctcgaaa cccggaagac ctcaagatgt 2701 agaatcctct ggaaccgagt tacagacagt tgtggattgt caaacacctt tgtgggcaag 2761 tccaatcacc actgccatgt gtctgcctat gaaaagtcat tccccatcaa gcctgcccca 2821 agtccgtcct ggagtgggtc gtgtcggcgg agtcttttaa gccccaagaa aactcagagg 2881 cgacatttca gcacagcaga agagacagtt caagaagaag aaaaggagat ctacaggcag 2941 ctgctgcaga tggtcacagg gaagcagttc tccgtagcca agcccaccac acatttccct 3001 ttacatctgt ctcggtgtct tagttccaat aagaactctt tgaaagactc actgttgaga 3061 aacgggaact cttgtgcatc tcatgtcatt ggttctgata cttcgtcctc tggatctgcc 3121 agcattttaa ctgcacagga acagttgtcc cacagtgcac attccctgat atctggtacc 3181 ccagatgttg cctttggatc caaagactcc gctcttcacc atcacctctc tgcacctcat 3241 cagccagaca gtttaccagc atcaaatcca cagtctgaag ggtcagactc tgtgatttta 3301 ctcaaagtga aagaatctca gacgccagct tccagatacc tcagtccatt gaagagaagt 3361 acttgcccct acattattcc ttattcagca gtcacggatc tgctctctag cagtcctact 3421 ttcttccagg cagagctttg gatcaaagaa ttaactagtg tttatgactc tcgagcccgg 3481 gaaagattgc gccagattga agagcagaag gcattggccc tacagcttca aaaccagaga 3541 ctgcaggagc aggagcatgc agtgcttgat tccgtagagc tccaccttcg tgttcctctg 3601 gaaaaggagg tccctgtcac agcagcccaa gaaaccagga aaaaaagtca ccaactaact 3661 gatagtgaag acgagttccc tgaaattaca gaggaaatgg agaaagaaat aaagaatgtg 3721 tttcgtaatg gtaaccagga tgaagtcctc agtgaggcct tccgcttgac catcacacgc 3781 aaagacattc agactctgaa ccatctaaac tggctcaatg acgagatcat caatttctac 3841 atgaatatgc taatggagcg cagtaaagag aagggatttc cgagtgtaca tgcctttaat 3901 acctttttct tcactaaact caaagcagct ggttatcagg cagtgaaacg ctggacaaag 3961 aaagtggatg tgttttctgt tgacattctc ctggtgccca ttcacttggg tgtgcactgg 4021 tgtctggcgg ttgtagactt tagaaagaag agtgtcacct actatgattc tatgggcgga 4081 gtaaacaatg aggcctgcag gatcctcttg caatacctaa aacaagaaag tgtcgacaag 4141 aaaaggaaag agtttgatac caatggctgg cagctcttca gcaagaaaag ccaggaaatc 4201 ccacagcaga tgaatggaag tgactgtgga atgtttgctt gcaaatatgc cgactgcatt 4261 accaaagaca gaccaatcaa ctttacacag caacacatgc catatttccg gaagcgaatg 4321 gtctgggaga tcctccaccg gaagctcttg ggatccGACT ACAAAGACCA TGACGGTGAT 4381 TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGtagcggcc gcGaaggatc 4441 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag 4501 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg 4561 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata 4621 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg 4681 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat 4741 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc 4801 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg 4861 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac 4921 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg 4981 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc 5041 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc 5101 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc 5161 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc 5221 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag 5281 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc 5341 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc 5401 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg 5461 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg 5521 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc 5581 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg 5641 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc 5701 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga 5761 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc 5821 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 5881 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 5941 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 6001 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 6061 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 6121 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 6181 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 6241 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 6301 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 6361 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac 6421 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 6481 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 6541 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 6601 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 6661 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 6721 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 6781 gtggtgttgt cggggaaATC ATCGTCCTTT CCTtggctgc tcgcctgtgt tgccacctgg 6841 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 6901 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tTcgccttcg ccctcagacg 6961 agtcggatct ccctttggGc cgcctccccg cctggtacct ttaagaccaa tgacttacaa 7021 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 7081 ctcccaacga aGataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 7141 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 7201 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 7261 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 7321 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 7381 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 7441 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 7501 gctctagcta TCCCGCCCCT AACTCCGCCC Atcccgcccc taactccgcc cagttccgcc 7561 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 7621 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga 7681 gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat 7741 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 7801 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 7861 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 7921 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 7981 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 8041 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 8101 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 8161 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 8221 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 8281 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 8341 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 8401 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 8461 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 8521 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 8581 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 8641 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 8701 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 8761 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 8821 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 8881 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 8941 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 9001 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 9061 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 9121 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 9181 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 9241 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 9301 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 9361 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 9421 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 9481 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 9541 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 9601 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 9661 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 9721 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 9781 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 9841 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 9901 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 9961 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 10021 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 10081 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 10141 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 10201 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 10261 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 10321 gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.