pLV3-CMV-STK19-3×HA-CopGFP-Pur

pLV3-CMV-STK19-3×HA-CopGFP-Puro plasmid

PVT89602

pLV3-CMV-STK19-3×HA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-STK19(human)-3×HA-CopGFP-Puro
Alias:
Gene lenght: 1104bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-STK19-3×HA-CopGFP-Puro plasmid Description

pLV3-CMV-STK19-3×HA-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-STK19-3×HA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgc aaaagtggtt ttctgctttc 2341 gatgatgcaa tcattcagcg acagtggcgg gcaaacccct cccggggcgg gggaggtgtg 2401 agcttcacga aggaggttga caccaacgtg gccaccggcg cccctccacg ccgccaacga 2461 gtccccgggc gtgcgtgccc ttggagggag ccaatccgcg gccggcgtgg ggcccggcct 2521 ggcggaggtg atgctggagg gacgcccggg gagaccgtac gtcactgctc tgcgccggaa 2581 gaccctattt tcaggttctc ttccctccat tcctacccct tccccggtac cataaaatcc 2641 cgggatatga gctggaagag gcatcacctg atcccggaga cctttggagt taagaggcgg 2701 cggaagcgag ggcctgtgga gtcggatcct cttcggggtg agccagggtc ggcgcgcgcg 2761 gctgtctcag aactcatgca gctgttcccg cgaggcctgt ttgaggacgc gctgccgccc 2821 atcgtgctga ggagccaggt gtacagcctt gtgcctgaca ggaccgtggc cgaccggcag 2881 ctgaaggagc ttcaagagca gggggagatc agaatcgtcc agctgggctt cgacttggat 2941 gcccatggaa ttatcttcac tgaggactac aggaccagag tatgtgactg tgtcctcaag 3001 gcctgtgatg gccgaccgta tgctggggca gtgcagaaat ttctagcttc agtacttcca 3061 gcctgtgggg accttagttt ccagcaggac caaatgacac agacctttgg cttcagggac 3121 tcagaaatca cgcatctggt gaatgctgga gtcctcaccg tccgagatgc tgggagctgg 3181 tggctagctg tgcctggagc tgggagattc atcaagtact ttgttaaagg gcgccaggct 3241 gtccttagca tggtccggaa ggcaaagtac cgggaactgc tcctatcaga gctcctgggc 3301 cggcgggcgc ctgtcgtggt gcggcttggc ctcacctacc atgtgcacga cctcattggg 3361 gcccagctag tggactgcat ctctaccact tcaggaaccc tcctccgcct gccagagaca 3421 CTCGAGTACC CATACGACGT ACCTGATTAT GCAACTTATC CTTATGATGT ACCTGATTAT 3481 GCTAGCTATC CTTATGATGT CCCAGACTAC GCATAGggat ccCTGCGATA TCCAGCgcgg 3541 ccgcGaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 3601 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc 3661 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 3721 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 3781 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc 3841 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc 3901 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg 3961 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt 4021 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga 4081 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag 4141 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga 4201 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg 4261 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc 4321 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac 4381 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac 4441 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc 4501 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg 4561 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac 4621 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg 4681 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag 4741 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc 4801 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc 4861 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc 4921 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 4981 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 5041 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 5101 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 5161 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 5221 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 5281 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 5341 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 5401 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 5461 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa 5521 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 5581 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 5641 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 5701 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 5761 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 5821 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 5881 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt 5941 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 6001 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctccgcctt 6061 cgccctcaga cgagtcggat ctccctttgg ccgcctcccc gcctggtacc tttaagacca 6121 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 6181 gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg 6241 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 6301 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 6361 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca 6421 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg 6481 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 6541 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 6601 tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc 6661 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 6721 tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa 6781 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 6841 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 6901 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 6961 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 7021 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 7081 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 7141 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 7201 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 7261 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 7321 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 7381 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 7441 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 7501 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 7561 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 7621 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 7681 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 7741 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 7801 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 7861 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 7921 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 7981 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 8041 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 8101 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 8161 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 8221 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 8281 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 8341 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 8401 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 8461 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 8521 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 8581 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 8641 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 8701 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 8761 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 8821 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 8881 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 8941 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 9001 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 9061 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 9121 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga 9181 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 9241 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc 9301 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt 9361 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.