pLV3-CMV-CPA4-3×FLAG-CopGFP-Pu

pLV3-CMV-CPA4-3×FLAG-CopGFP-Puro plasmid

PVT89094

pLV3-CMV-CPA4-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-CPA4(human)-3×FLAG-CopGFP-Puro
Alias: NM_016352.4;CPA3;
Gene lenght: 1263bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CPA4-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-CPA4-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-CPA4-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGGATATC gccaccatga ggtggatact gttcattggg 2341 gcccttattg ggtccagcat ctgtggccaa gaaaaatttt ttggggacca agttttgagg 2401 attaatgtca gaaatggaga cgagatcagc aaattgagtc aactagtgaa ttcaaacaac 2461 ttgaagctca atttctggaa atctccctcc tccttcaatc ggcctgtgga tgtcctggtc 2521 ccatctgtca gtctgcaggc atttaaatcc ttcctgagat cccagggctt agagtacgca 2581 gtgacaattg aggacctgca ggccctttta gacaatgaag atgatgaaat gcaacacaat 2641 gaagggcaag aacggagcag taataacttc aactacgggg cttaccattc cctggaagct 2701 atttaccacg agatggacaa cattgccgca gactttcctg acctggcgag gagggtgaag 2761 attggacatt cgtttgaaaa ccggccgatg tatgtactga agttcagcac tgggaaaggc 2821 gtgaggcggc cggccgtttg gctgaatgca ggcatccatt cccgagagtg gatctcccag 2881 gccactgcaa tctggacggc aaggaagatt gtatctgatt accagaggga tccagctatc 2941 acctccatct tggagaaaat ggatattttc ttgttgcctg tggccaatcc tgatggatat 3001 gtgtatactc aaactcaaaa ccgattatgg aggaagacgc ggtcccgaaa tcctggaagc 3061 tcctgcattg gtgctgaccc aaatagaaac tggaacgcta gttttgcagg aaagggagcc 3121 agcgacaacc cttgctccga agtgtaccat ggaccccacg ccaattcgga agtggaggtg 3181 aaatcagtgg tagatttcat ccaaaaacat gggaatttca agggcttcat cgacctgcac 3241 agctactcgc agctgctgat gtatccatat gggtactcag tcaaaaaggc cccagatgcc 3301 gaggaactcg acaaggtggc gaggcttgcg gccaaagctc tggcttctgt gtcgggcact 3361 gagtaccaag tgggtcccac ctgcaccact gtctatccag ctagcgggag cagcatcgac 3421 tgggcatatg acaacggcat caaatttgca ttcacatttg agttgagaga taccgggacc 3481 tatggcttcc tcctgccagc taaccagatc atccccactg cagaggagac gtggctgggg 3541 ctgaagacca tcatggagca tgtgcgggac aacctctacC TCGAGGACTA CAAAGACCAT 3601 GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA Gtagcggccg 3661 cGaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc 3721 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg 3781 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa 3841 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga 3901 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga 3961 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga 4021 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg 4081 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct 4141 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg 4201 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc 4261 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag 4321 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc 4381 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac 4441 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc 4501 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag 4561 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg 4621 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc 4681 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac 4741 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag 4801 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg 4861 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct 4921 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag 4981 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag 5041 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 5101 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 5161 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 5221 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 5281 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 5341 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 5401 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 5461 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 5521 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 5581 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct 5641 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 5701 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 5761 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 5821 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 5881 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 5941 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 6001 gacaattccg tggtgttgtc ggggaaATCA TCGTCCTTTC CTtggctgct cgcctgtgtt 6061 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 6121 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct Tcgccttcgc 6181 cctcagacga gtcggatctc cctttggGcc gcctccccgc ctggtacctt taagaccaat 6241 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 6301 gctaattcac tcccaacgaa Gataagatct gctttttgct tgtactgggt ctctctggtt 6361 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 6421 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 6481 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 6541 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 6601 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 6661 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 6721 tcatgtctgg ctctagctaT CCCGCCCCTA ACTCCGCCCA tcccgcccct aactccgccc 6781 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 6841 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac 6901 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc 6961 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 7021 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 7081 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 7141 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 7201 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 7261 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 7321 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 7381 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 7441 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 7501 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 7561 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 7621 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 7681 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 7741 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 7801 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 7861 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 7921 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 7981 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 8041 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 8101 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 8161 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 8221 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 8281 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 8341 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 8401 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 8461 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 8521 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 8581 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 8641 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 8701 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 8761 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 8821 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 8881 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 8941 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 9001 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 9061 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 9121 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 9181 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 9241 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 9301 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 9361 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 9421 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 9481 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 9541 cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.