PH-PLCD1(R40L)-GFP plasmid

PH-PLCD1(R40L)-GFP plasmid

PVT88899

PH-PLCD1(R40L)-GFP plasmid Information

Plasmid name: PH-PLCD1(R40L)-GFP
Alias: Plasmid #51408
Gene lenght: 510bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

PH-PLCD1(R40L)-GFP plasmid Description

PH-PLCD1(R40L)-GFP is a plasmid suitable for protein expression in mammalian cells.

PH-PLCD1(R40L)-GFP plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcacggca tggactcggg ccgggacttc 661 ctgaccctgc acggcctaca ggatgatgag gatctacagg cgctgctgaa gggcagccag 721 ctcctgaagg tgaagtcGaC ctcatggagg agagagcTGt tctacaagtt gcaggaggac 781 tgcaagacca tctggcagga gtcccgcaag gtcatgcgga ccccggagtc ccagctgttc 841 tccatcgagg acattcagga ggtgcgaatg gggcaccgca cggagggtct ggagaagttc 901 gcccgtgatg tgcccgagga ccgctgcttc tccattgtct tcaaggacca gcgcaataca 961 ctagacctca tcgccccatc gccagctgat gcccagcact gggtgctggg gctgcacaag 1021 atcatccacc actcaggctc catggaccag cgtcagaagc tacagcactg gattcactcc 1081 tgcttgcgaa aagctgacaa aaacaaggac aacaagatga gcttcaagga gctgcagaac 1141 ttcctgaagg atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg 1201 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc 1261 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc 1321 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc 1381 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa 1441 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc 1501 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc 1561 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc 1621 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac 1681 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac 1741 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac 1801 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact 1861 ctcggcatgg acgagctgta caagtaaagc ggccgcgact ctagatcata atcagccata 1921 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga 1981 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca 2041 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 2101 gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg 2161 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 2221 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 2281 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 2341 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg 2401 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 2461 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 2521 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 2581 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 2641 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 2701 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc 2761 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 2821 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 2881 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 2941 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 3001 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 3061 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg 3121 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc 3181 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg 3241 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa 3301 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc 3361 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat 3421 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg 3481 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc 3541 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag 3601 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc 3661 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc 3721 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata 3781 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc 3841 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac 3901 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc 3961 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt 4021 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc 4081 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta 4141 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg 4201 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat 4261 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc 4321 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt 4381 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 4441 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 4501 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 4561 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 4621 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtTctt ctagtgtagc 4681 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 4741 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 4801 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 4861 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 4921 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 4981 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 5041 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 5101 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 5161 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc 5221 at

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.