pCDFDuet-AtUBA1-S plasmid

pCDFDuet-AtUBA1-S plasmid

PVT88802

pCDFDuet-AtUBA1-S plasmid Information

Plasmid name: pCDFDuet-AtUBA1-S
Alias:
Gene lenght: 3240bp
Host: bacteria
Use(s): Protein Expression
Fragment type: ORF
Species: Arabidopsis thaliana
Prokaryotic resistance: Str
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: CloDF13
Growth Strain(s): DH5a
Growth Temperature:
Vector backbone:
Forward primer:
Reverse primer: LacI-R
Copy number: High
Induction: IPTG Induction

pCDFDuet-AtUBA1-S plasmid Description

pCDFDuet-AtUBA1-S This is a plasmid suitable for protein expression in bacteria.

pCDFDuet-AtUBA1-S plasmid Sequence

1 gggatctcga cgctctccct tatgcgactc ctgcattagg aaattaatac gactcactat 61 aggggaattg tgagcggata acaattcccc tgtagaaata attttgttta actttaatAA 121 GGAGatatac catgggcagc agccatcacc atcatcacca cagccaggga tccgaattcg 181 agctcggcgc gcctgcaggt cgacaagctt gcggccgcat aatgcttaag tcgaacagaa 241 agtaatcgta ttgtacacgg ccgcataatc gaaattaata cgactcacta taggggaatt 301 gtgagcggat aacaattccc catcttagta tattagttaa gtataagAAG GAGatataca 361 tatggcagat ctcaattgga tatcgATGCT TCACAAGCGA GCTAGTGAAG CTAACGATAA 421 GAACGATAAT ACCATCATCG GTTCCGATTT AGCATCATCT AAGAAACGCC GTATCGATTT 481 CACTGAATCT TCGTCTGATA AGTCTTCTTC GATTTTAGCT TCTGGTAGTA GCAGGGGTTT 541 TCACGGCGAT AGCGTCGTTC AGCAAATCGA CATGGCTTTT GGTAATTCGA ACCGTCAGGA 601 GATTGATGAA GATCTGCACA GTCGCCAGCT CGCCGTCTAT GGTCGTGAGA CTATGAGGCG 661 TCTCTTTGCT TCCAATGTTC TCATCTCGGG GATGCACGGT CTTGGTGCCG AGATTGCCAA 721 GAATCTTATA CTTGCTGGTG TGAAGTCTGT GACCTTGCAT GATGAAAGAG TTGTAGAGCT 781 ATGGGACTTA TCAAGCAACT TTGTTTTCTC GGAGGATGAT GTTGGCAAGA ATAGAGCAGA 841 TGCTTCTGTT CAGAAGTTAC AGGATCTTAA CAATGCTGTG GTTGTCTCTA GCTTGACCAA 901 AAGCTTAAAC AAAGAGGATC TTTCTGGCTT CCAGGTTGTT GTATTCTCTG ACATAAGCAT 961 GGAAAGAGCA ATCGAATTTG ATGACTATTG TCACAGCCAT CAGCCTCCTA TCGCTTTTGT 1021 CAAGGCTGAT GTCAGGGGTC TTTTCGGCTC TGTCTTTTGT GATTTTGGGC CTGAATTTGC 1081 GGTTCTTGAT GTTGATGGAG AGGAACCCCA TACAGGCATC ATTGCGTCTA TCTCTAATGA 1141 GAACCAGGCA TTTATTTCCT GTGTCGACGA TGAGAGGCTT GAGTTTGAAG ATGGTGACCT 1201 AGTTGTTTTC TCAGAAGTTG AGGGTATGAC AGAATTGAAT GATGGGAAAC CAAGGAAGAT 1261 AAAAAGTACA CGGCCATATT CATTCACCCT TGATGAGGAC ACTACTAACT ATGGAACATA 1321 TGTGAAGGGT GGTATTGTCA CTCAAGTGAA ACAGCCAAAG TTACTGAATT TCAAGCCATT 1381 GAGGGAAGCG CTTAAGGATC CAGGGGATTT TCTATTTAGT GATTTCTCTA AGTTTGATCG 1441 GCCTCCACTC CTTCACTTAG CATTCCAGGC ACTTGATCAT TTTAAAGCTG AAGCTGGCAG 1501 ATTTCCTGTT GCTGGCTCAG AAGAGGACGC TCAGAAGCTT ATATCGATTG CCACAGCCAT 1561 CAATACCGGG CAGGGTGATT TAAAGGTGGA GAATGTTGAT CAGAAGCTTC TAAGACACTT 1621 TTCCTTTGGA GCCAAGGCTG TCCTGAATCC TATGGCTGCA ATGTTTGGCG GTATTGTTGG 1681 ACAGGAGGTT GTTAAAGCTT GCTCTGGAAA ATTTCATCCT CTCTTCCAGT TTTTCTACTT 1741 TGATTCAGTG GAGTCACTCC CCTCTGAACC TGTGGACTCG AGTGACTTTG CACCAAGAAA 1801 CAGCCGGTAT GATGCCCAAA TATCTGTATT TGGGGCCAAG TTCCAGAAGA AACTTGAAGA 1861 TGCTAAAGTT TTCACAGTAG GGTCTGGTGC TCTTGGCTGT GAGTTCTTGA AAAATTTGGC 1921 TCTGATGGGG GTTTCTTGTG GAAGCCAAGG GAAGCTGACA GTGACAGATG ATGATATCAT 1981 TGAGAAGAGT AACCTCAGTC GTCAATTTCT GTTCCGTGAC TGGAACATTG GACAGGCTAA 2041 ATCCACAGTT GCTGCTTCGG CTGCTGCTGT TATAAATCCT CGCTTCAACA TTGAGGCCCT 2101 GCAAAACCGT GTTGGCGCGG AGACTGAGAA TGTATTTGAC GATGCCTTCT GGGAGAACTT 2161 AACTGTTGTC GTCAATGCAC TGGATAATGT TAATGCGAGG CTCTATGTTG ATTCGAGGTG 2221 CTTGTATTTC CAGAAGCCTC TCCTTGAGTC TGGAACTCTT GGTACAAAGT GCAACACACA 2281 GTCGGTTATC CCACATCTAA CTGAAAATTA CGGTGCCTCA AGGGACCCAC CAGAGAAACA 2341 GGCCCCCATG TGTACAGTGC ATTCCTTCCC GCATAACATT GATCACTGTT TAACTTGGGC 2401 TCGCTCTGAG TTTGAGGGTC TGCTGGAGAA GACTCCCGCT GAAGTGAATG CATATCTTTC 2461 TAGCCCAGTT GAGTACACTA ACTCAATGAT GAGTGCTGGT GATGCTCAGG CGAGGGACAC 2521 ATTGGAGAGG ATTGTTGAGT GCCTTGAAAA GGAGAAGTGT GAGACCTTCC AAGACTGCTT 2581 AACCTGGGCA CGACTGAGGT TTGAGGATTA CTTTGTGAAC CGTGTCAAGC AATTGATATA 2641 CACATTTCCT GAAGATGCTG CGACTAGTAC CGGAGCTCCA TTCTGGTCTG CTCCAAAAAG 2701 ATTCCCTCGT CCACTCCAGT ACTCGTCGTC CGACCCAAGT CTCCTTAACT TTATCACAGC 2761 GACTGCTATT TTACGAGCAG AGACATTTGG GATCCCGATA CCTGAGTGGA CTAAGAACCC 2821 AAAGGAAGCA GCAGAAGCTG TTGACAGAGT GATAGTCCCA GACTTTGAGC CAAGGCAAGA 2881 TGCAAAGATT GTGACTGATG AGAAAGCTAC CACTTTAACC ACTGCTTCGG TGGACGACGC 2941 TGCAGTCATC GACGATCTCA TTGCTAAGAT CGACCAGTGT AGGCATAACT TGTCGCCAGA 3001 CTTCAGGATG AAACCTATTC AGTTCGAAAA GGATGATGAT ACAAACTATC ACATGGATGT 3061 GATAGCAGGA CTAGCCAACA TGAGGGCGAG AAATTACAGC ATCCCTGAAG TGGACAAGCT 3121 GAAAGCAAAG TTCATAGCAG GGAGAATCAT CCCAGCCATT GCGACTTCAA CAGCCATGGC 3181 TACTGGGCTG GTCTGCCTCG AGCTGTACAA GGTCCTTGAT GGAGGACATA AAGTGGAAGC 3241 TTACAGAAAC ACATTTGCCA ACCTGGCACT TCCACTATTC TCCATGGCTG AACCACTTCC 3301 ACCAAAGGTG GTGAAGCACC GTGACATGGC TTGGACAGTT TGGGACAGAT GGGTTCTCAA 3361 GGGAAACCCA ACTCTTCGTG AGGTGTTGCA GTGGCTGGAG GACAAAGGGC TTAGCGCATA 3421 CAGCATCTCT TGTGGAAGCT GTCTTCTGTT CAACAGTATG TTCACAAGGC ACAAGGAGAG 3481 GATGGACAAG AAGGTTGTGG ATCTCGCTCG GGATGTTGCG AAAGTCGAGT TACCGCCTTA 3541 TCGCAACCAT CTTGATGTAG TGGTGGCTTG TGAGGATGAA GATGACAATG ACGTCGATAT 3601 TCCTCTCGTC TCTATCTACT TCAGGggtac cctcgagtct ggtaaagaaa ccgctgctgc 3661 gaaatttgaa cgccagcaca tggactcgtc tactagcgca gcttaattaa cctaggctgc 3721 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg 3781 ttttttgctg aaacctcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa 3841 taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac 3901 gaccgggtca tcgtggccgg atcttgcggc ccctcggctt gaacgaattg ttagacatta 3961 tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc 4021 tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat 4081 gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg 4141 cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg 4201 ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc 4261 aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc 4321 aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg 4381 ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt 4441 agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg 4501 gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg 4561 ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg 4621 cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag 4681 atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc 4741 ttccctcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 4801 gagcggatac atatttgaat gtatttagaa aaataaacaa atagctagct cactcggtcg 4861 ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca catacaaagt tacccacaga 4921 ttccgtggat aagcagggga ctaacatgtg aggcaaaaca gcagggccgc gccggtggcg 4981 tttttccata ggctccgccc tcctgccaga gttcacataa acagacgctt ttccggtgca 5041 tctgtgggag ccgtgaggct caaccatgaa tctgacagta cgggcgaaac ccgacaggac 5101 ttaaagatcc ccaccgtttc cggcgggtcg ctccctcttg cgctctcctg ttccgaccct 5161 gccgtttacc ggatacctgt tccgcctttc tcccttacgg gaagtgtggc gctttctcat 5221 agctcacaca ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg ggctgtaagc 5281 aagaactccc cgttcagccc gactgctgcg ccttatccgg taactgttca cttgagtcca 5341 acccggaaaa gcacggtaaa acgccactgg cagcagccat tggtaactgg gagttcgcag 5401 aggatttgtt tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt ccggctacac 5461 tggaaggaca gatttggttg ctgtgctctg cgaaagccag ttaccacggt taagcagttc 5521 cccaactgac ttaaccttcg atcaaaccac ctccccaggt ggttttttcg tttacagggc 5581 aaaagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctactgaac 5641 cgctctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc agccccatac 5701 gatataagtt gtaattctca tgttagtcat gccccgcgcc caccggaagg agctgactgg 5761 gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac 5821 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 5881 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt 5941 ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga 6001 gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg 6061 ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatgt 6121 ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat 6181 cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt 6241 gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc 6301 gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc 6361 ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg 6421 taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa 6481 ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg 6541 gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac 6601 aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg 6661 cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg 6721 caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt 6781 aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg 6841 cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt 6901 ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg 6961 ccataccgcg aaaggttttg cgccattcga tggtgtcc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.