pLV2-U6-Zeb2-shRNA5-ZsGreen-Pu

pLV2-U6-Zeb2-shRNA5-ZsGreen-Puro plasmid

PVT88548

pLV2-U6-Zeb2-shRNA5-ZsGreen-Puro plasmid Information

Plasmid name: pLV2-U6-Zeb2(mouse)-shRNA5-ZsGreen-Puro
Alias: GCCACGATCCAGACCACAATT
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV2-U6-Zeb2-shRNA5-ZsGreen-Puro plasmid Description

pLV2-U6-Zeb2-shRNA5-ZsGreen-Puro This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV2-U6-Zeb2-shRNA5-ZsGreen-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccTTT AAAAGAAAAG 2041 GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC 2101 AAACTAAAGA ATTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG 2161 ACAGCAGAGA TCCAGTTTAT CGATCTGGGC AGGAAGAGGG CCTATTTCCC ATGATTCCTT 2221 CATATTTGCA TATACGATAC AAGGCTGTTA GAGAGATAAT TAGAATTAAT TTGACTGTAA 2281 ACACAAAGAT ATTAGTACAA AATACGTGAC GTAGAAAGTA ATAATTTCTT GGGTAGTTTG 2341 CAGTTTTAAA ATTATGTTTT AAAATGGACT ATCATATGCT TACCGTAACT TGAAAGTATT 2401 TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGG ATCCGGCCAC GATCCAGACC 2461 ACAATTctcg agAATTGTGG TCTGGATCGT GGCtTTTTTG AATTCTACCg ctccggtgcc 2521 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 2581 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 2641 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 2701 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc 2761 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacgccc 2821 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 2881 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcttgggcg 2941 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 3001 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 3061 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 3121 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 3181 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 3241 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 3301 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 3361 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 3421 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 3481 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 3541 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 3601 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 3661 aagttttttt cttccatttc aggtgtcgtg attcACCTGC AGCCCAAGCT TACCGCCACC 3721 ATGGCCCAGT CCAAGCACGG CCTGACCAAG GAGATGACCA TGAAGTACCG CATGGAGGGC 3781 TGCGTGGACG GCCACAAGTT CGTGATCACC GGCGAGGGCA TCGGCTACCC CTTCAAGGGC 3841 AAGCAGGCCA TCAACCTGTG CGTGGTGGAG GGCGGCCCCT TGCCCTTCGC CGAGGACATC 3901 TTGTCCGCCG CCTTCATGTA CGGCAACCGC GTGTTCACCG AGTACCCCCA GGACATCGTC 3961 GACTACTTCA AGAACTCCTG CCCCGCCGGC TACACCTGGG ACCGCTCCTT CCTGTTCGAG 4021 GACGGCGCCG TGTGCATCTG CAACGCCGAC ATCACCGTGA GCGTGGAGGA GAACTGCATG 4081 TACCACGAGT CCAAGTTCTA CGGCGTGAAC TTCCCCGCCG ACGGCCCCGT GATGAAGAAG 4141 ATGACCGACA ACTGGGAGCC CTCCTGCGAG AAGATCATCC CCGTGCCCAA GCAGGGCATC 4201 TTGAAGGGCG ACGTGAGCAT GTACCTGCTG CTGAAGGACG GTGGCCGCTT GCGCTGCCAG 4261 TTCGACACCG TGTACAAGGC CAAGTCCGTG CCCCGCAAGA TGCCCGACTG GCACTTCATC 4321 CAGCACAAGC TGACCCGCGA GGACCGCAGC GACGCCAAGA ACCAGAAGTG GCACCTGACC 4381 GAGCACGCCA TCGCCTCCGG CTCCGCCTTG CCCGAATTTG GCAGCGGCCA GTGCACCAAC 4441 TATGCCCTGC TGAAACTGGC TGGCGATGTG GAAAGCAACC CCGGACCCAT GACCGAGTAC 4501 AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC 4561 GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG 4621 CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG 4681 TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG 4741 GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC 4801 GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC 4861 CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG 4921 CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG 4981 CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG 5041 CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGACG CCCGCCCCAC 5101 GACCCGCAGC GCCCGACCGA AAGGAGCGCA CGACCCCATG CATCGGCGTC TCGAGTCTAG 5161 AGGGCCCGAC GCGTCTGGAA CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT 5221 GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGGCTTTG 5281 TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG 5341 CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG 5401 TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG 5461 ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC 5521 TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG 5581 ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC 5641 TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT 5701 CTGCGGCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC 5761 TCCCCGCCTG GAATTAATTC TGCAGTCGAG ACCTAGAAAA ACATGGAGCA ATCACAAGTA 5821 GCAATACAGC AGCTATCAAT GATTGTGCCT GGCTAGAAGC ACAAGAGGAG GAGGAGGTGG 5881 GTTTTCCAGT CACACCTCAG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC 5941 TTAGCCACTT TTTAAAAGAA AAGAGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC 6001 AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TAGCAGAACT 6061 ACACACCAGG GCCAGGGGTC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC 6121 CAGTTGAGCC AGATAAGGTA GAAGAGGCCA ATAAAGGAGA GAACACCAGC TTGTTACACC 6181 CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT GTTAGAGTGG AGGTTTGACA 6241 GCCGCCTAGC ATTTCATCAC GTGGCCCGAG AGCTGCATCC GGAGTACTTC AAGAACTGCT 6301 GATATCGAGC TTGCTACAAG GGACTTTCCG CTGGGGACTT TCCAGGGAGG CGTGGCCTGG 6361 GCGGGACTGG GGAGTGGCGA GCCCTCAGAT CCTGCATATA AGCAGCTGCT TTTTGCCTGT 6421 ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC 6481 CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG 6541 TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT 6601 AGCAGTAGTA GTTCATGTCA TCTTATTATT CAGTATTTAT AACTTGCAAA GAAATGAATA 6661 TCAGAGAGTG AGAGGCCTTG ACATTGCTAG CGTTTACCGT CGACCTCTAG CTAGAGCTTG 6721 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC 6781 AACATACGAG CCGGAAGCAT AAAGTGTAAA gcctggggtg cctaatgagt gagctaactc 6841 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 6901 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 6961 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 7021 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 7081 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 7141 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 7201 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 7261 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 7321 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 7381 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 7441 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 7501 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 7561 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 7621 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 7681 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 7741 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 7801 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 7861 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 7921 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 7981 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 8041 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 8101 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 8161 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 8221 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 8281 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 8341 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 8401 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 8461 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 8521 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 8581 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 8641 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 8701 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 8761 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 8821 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 8881 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata 8941 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 9001 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa 9061 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt 9121 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg 9181 ttaaatatgt actacaaact tagtagtttt taaagaaatt gtatttgtta aatatgtact 9241 acaaacttag tagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.