pLV3-CMV-Baz1b-3×FLAG-CopGFP-P

pLV3-CMV-Baz1b-3×FLAG-CopGFP-Puro plasmid

PVT88147

pLV3-CMV-Baz1b-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Baz1b(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_011714.2;Wbscr9; WSTF
Gene lenght: 4437bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Baz1b-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Baz1b-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Baz1b-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cgccgctgct gggccgcaag 2341 cccttcccgc tggtgaaacc gctgcccgga gaggagccgc tcttcaccat cccgcacact 2401 caggaggcct tccgcacccg ggaagagtat gaagcccgcc tggaaagata cagtgaacgc 2461 atttggacgt gtaagagtac aggaagcagt cagctaacac acaaggaggc ctgggaagag 2521 gaacaggaag ttgctgagct tttgaaggaa gagtttccta actggtatga gaagcttgtt 2581 ttggaaatgg ttcaccataa cacagcctcc ttagagaagc ttgtcgactc tgcctggtta 2641 gagatcatga ccaaatatgc cgtgggggaa gagtgtgact ttgaggttgg gaaggagaaa 2701 atgctcaagg tgaagatagt gaagattcat cctttggaga aagttgatga agaagctgtt 2761 gagaagaaat ctgatggggc ctgtgattct ccgtcaagtg acaaagaaaa ttccagtcaa 2821 atggctcagg acctccagaa gaaggagaca gtagtcaaag aggatgaagg caggagagag 2881 agtattaatg acagagcgcg taggtctcca cgaaaacttc ctacttcatt aaaaaaagga 2941 gaaagaaaat gggctcctcc aaaatttctg cctcacaaat atgatgtgaa gctacaaaat 3001 gaagataaga tcatcagcaa tgtgccagca gacagcttga ttcgaacgga gagaccacca 3061 aataaggaga tacttcgata ctttattcgg cataatgcct tacgtgctgg tactggtgag 3121 aatgcacctt gggtggtgga agatgaattg gtgaagaaat attccctgcc tagcaagttc 3181 agtgactttt tacttgaccc atataagtat atgactctca atccctctac caagaggagg 3241 aatactggat ctccagacag gaagccctca aagaaaccta agagagacag ttcttctctg 3301 agttccccac taaaccctaa gctgtggtgt catgtgcact tggagaagtc gttaaatggc 3361 ccaccactca aagtaaagaa ctcaaagaat tccaagtctc cggaagaaca tttggaaggg 3421 gtgatgaaaa ttatgtcccc caacaacaac aagctacaca gcttccacat tcctaagaaa 3481 ggcccagccg ccaagaagcc agggaagcac agtgacaaac ctctgaaggc caagggcaga 3541 ggcaaaggca tcctgaatgg acagaagtcc acaggcaact ccaaatcacc cagcaagtgt 3601 gtgaagactc ctaagaccaa aatgaagcag atgactttgt tggatatggc caaagggact 3661 cagaagatga cccgcacccc acggagttct gggggtgtgc caaggtcttc tggtaaacct 3721 cacaaacacc tgcctcctgc agctctgcat ctgattgcct attacaaaga aaataaagac 3781 aaagaggaca agaagagtgc tctgtcctgt gttatctcca aaacagcacg tctcctctcc 3841 aatgaagata gagcccgtct tccagaagag ctccgggctc ttgtccagaa acgctatgag 3901 ctcctggaac ataaaaagag gtgggcctct atgtctgaag agcaacggaa agaatatttg 3961 aaaaagaaac gacaggagct aaaggagagg ttgcgggaaa aagccaaaga acggagagag 4021 agagaaatgc tggagaggtt agaaaagcag aagcgatttg aggaccagga gttaggtggc 4081 agaaacctcc cagcattcag gctggtagat acccctgagg ggctccccaa cactcttttt 4141 ggggatgtgg ccctggtggt ggagttcctg agctgttact ctgggttgct cttgcccgat 4201 gctcagtacc ctatcactgc tgtgtcccta atggaagcct tgagtgcaga taaaggtggt 4261 tttttgtact tgaacagagt gctggtcatc ctgctacaga ccttacttca ggatgaaatt 4321 gcagaagact atggagaact gggaatgaag ctgtcagaaa tccccctcac cctgcattct 4381 gtctcagagc tggtccggct ctgcttgcgc aggtgtgatg tccaggagga cagcgagggc 4441 tcagaaacag atgacaataa agattccaca ccatttgagg ataatgaggt gcaagacgaa 4501 tttctagaga agctggagac atcagaattc tttgagctga cctcagagga gaagctacgg 4561 atattgacag ctctttgcca ccgtatcctc atgacatact ctgtgcaaga ccacatggag 4621 acaagacagc aggtgtctgc agagttgtgg aaggagcggc ttgctgtgct gaaggaggag 4681 aatgataaga agagagcaga gaagcaaaag cggaaggaga tggaagccag aaataaagaa 4741 aatggcaaag aggagaatgt gctaggcaaa gtggacagga aaaaagaaat tgtgaagatt 4801 gaacagcaag tagaagtgga agcagatgac atgatcagtg ctgtcaagag cagaaggctg 4861 cttagcatgc aagctaagag gaagcgggaa atccaggaga gagaaacaaa agtgagattg 4921 gaacgggaag ctgaagagga acgaatgcgg aaacacaagg ctgctgccga gaaggctttc 4981 caggaaggga ttgccaaggc aaaacttgta ctgcgcagga cacctattgg tacagaccgc 5041 aaccataaca gatactggct cttctcaaac gaagtcccag gattattcat tgaaaagggc 5101 tgggtccata atagcattga ctaccggttt aagcatcacc gcaaagacca cagcaattta 5161 cctgatgatg actactgtcc tcgaagtaag aaagcaaacc taggcaagaa tgcaagtgtg 5221 aatgcgcacc atggaccagc actagaagct gtggagacca ctgtgcccaa gcaaggacaa 5281 aacttatggt tcctgtgtga cagtcagaag gaattggatg agctgctgag ttgcctccat 5341 cctcagggaa tcagggaaag tcagcttaaa gagaggctgg agaagagata ccaagaaatt 5401 actcattcta tttatctggc tcggaagcca aatttgggtc taaaatcttg tgacggcaac 5461 caggagctct taaacttcct gaggagtgat cttattgaag tggcaacgag gctgcagaaa 5521 ggaggacttg gctacatgga gggaacatct gagtttgagg cccgggtcat ttccttagag 5581 aaactgaagg attttggtga atgcgtcatt gcccttcaag ccagtgtcat aaagaaattt 5641 ctgcaaggat ttatggcgcc taagcagaag aagcggaaac tccaaagtga agattccaca 5701 aagtccgagg aagtagatga agagaaaaag atggtagagg aagcaaaggt tgcctctgca 5761 ctggaaaaat ggaaaacagc aatccgtgaa gcacagacct tttccaggat gcatgttcta 5821 cttgggatgc tcgatgcatg tatcaagtgg gacatgtcag cagaaaacgc taggtgcaaa 5881 gtttgtcgaa agaaaggtga ggatgacaag ctgatcctgt gtgacgagtg taataaagcc 5941 ttccacctgt tctgtctgag gccggccctt tatgaagtac cagatggcga gtggcagtgt 6001 ccagcttgcc agcctcctac tgccagacgc aattcccgtg gcaggaatta cactgaagag 6061 tctacctctg agggcagcga gggtgatgag agcggtgaag aggaagagga agaagaggag 6121 gaggaggaag aagaagagga ttatgaagtg gctggcttgc ggctgagacc tcggaagacc 6181 attcgaggca agcagagtgt catccctgca gcaagaccag gccgaccgcc gggtaagaag 6241 tcacatcctg ccagaaggtc acggccaaag gatgacccag aggtcgatga tctggtactt 6301 cagaccaagc gtatctcgag aaggcagagt ctggagctac aaaagtgtga ggatatcctt 6361 cacaaactgg tgaagtaccg cttcagctgg cccttccggg agcctgtgac cagagacgag 6421 gctgaggact actatgatgt gatcgagcac cccatggact tccagaccat acagaacaaa 6481 tgctcatgcg ggaactaccg atctgtgcag gagttcctca ctgacatgaa gcaagtgttt 6541 gccaatgccg agctctataa ctgccgcggc agccacgtgc tgagttgcat ggagaagaca 6601 gagcagtgtc tgctggctct gctgcaaaaa cacctccccg gccaccccta cgtgcgcagg 6661 aaacgcagga agtttcctga taggctcgct gatgatgaag gggacagtga ctcagagtct 6721 gttggacagt ccaggggacg gagacagaag aagggatccG ACTACAAAGA CCATGACGGT 6781 GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGtagcg gccgcGaagg 6841 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 6901 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac 6961 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 7021 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 7081 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc 7141 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg 7201 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct 7261 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc 7321 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct 7381 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc 7441 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc 7501 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc 7561 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc 7621 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc 7681 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc 7741 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc 7801 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac 7861 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc 7921 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg 7981 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc 8041 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg 8101 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga 8161 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag 8221 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 8281 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 8341 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 8401 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 8461 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 8521 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 8581 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 8641 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 8701 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 8761 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat 8821 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 8881 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 8941 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 9001 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 9061 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 9121 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 9181 tccgtggtgt tgtcggggaa ATCATCGTCC TTTCCTtggc tgctcgcctg tgttgccacc 9241 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 9301 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctTcgcct tcgccctcag 9361 acgagtcgga tctccctttg gGccgcctcc ccgcctggta cctttaagac caatgactta 9421 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 9481 tcactcccaa cgaaGataag atctgctttt tgcttgtact gggtctctct ggttagacca 9541 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 9601 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 9661 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 9721 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 9781 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 9841 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 9901 ctggctctag ctaTCCCGCC CCTAACTCCG CCCAtcccgc ccctaactcc gcccagttcc 9961 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 10021 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc 10081 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac 10141 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 10201 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 10261 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 10321 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 10381 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 10441 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 10501 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 10561 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 10621 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 10681 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 10741 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 10801 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 10861 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 10921 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 10981 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 11041 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 11101 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 11161 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 11221 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 11281 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 11341 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 11401 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 11461 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 11521 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 11581 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 11641 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 11701 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 11761 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 11821 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 11881 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 11941 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 12001 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 12061 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 12121 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 12181 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 12241 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 12301 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 12361 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 12421 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 12481 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 12541 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 12601 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 12661 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 12721 caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.