pT7-6×His-NELL1-6×His plasmid

pT7-6×His-NELL1-6×His plasmid

PVT87640

pT7-6×His-NELL1-6×His plasmid Information

Plasmid name: pT7-6×His-NELL1(human)-6×His
Alias: NM_006157.5;IDH3GL; NRP1
Gene lenght: 2430bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pET-28a
Forward primer: T7
Reverse primer:
Copy number: High
Induction:

pT7-6×His-NELL1-6×His plasmid Description

pT7-6×His-NELL1-6×His This is a prokaryotic inducible low-copy expression plasmid. The plasmid contains a T7-lacO promoter system, which induces the expression of the T7 promoter-driven human NELL1 gene in the presence of IPTG or lactose and its analogs. The N-terminal and C-terminal of the NELL1 gene in the plasmid carry a 6×His tag, facilitating the detection and purification of the fusion protein.

pT7-6×His-NELL1-6×His plasmid Sequence

1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 5041 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac 5101 agcagcggcc tggtgccgcg cggcagccat atggctagca tgactggtgg acagcaaatg 5161 ggtcgcggat ccatgccgat ggatttgatt ttagttgtgt ggttctgtgt gtgcactgcc 5221 aggacagtgg tgggctttgg gatggaccct gaccttcaga tggatatcgt caccgagctt 5281 gaccttgtga acaccaccct tggagttgct caggtgtctg gaatgcacaa tgccagcaaa 5341 gcatttttat ttcaagacat agaaagagag atccatgcag ctcctcatgt gagtgagaaa 5401 ttaattcagc tgttccggaa caagagtgaa ttcaccattt tggccactgt acagcagaag 5461 ccatccactt caggagtgat actgtccatt cgagaactgg agcacagcta ttttgaactg 5521 gagagcagtg gcctgaggga tgagattcgg tatcactaca tacacaatgg gaagccaagg 5581 acagaggcac ttccttaccg catggcagat ggacaatggc acaaggttgc actgtcagtt 5641 agcgcctctc atctcctgct ccatgtcgac tgtaacagga tttatgagcg tgtgatagac 5701 cctccagata ccaaccttcc cccaggaatc aatttatggc ttggccagcg caaccaaaag 5761 catggcttat tcaaagggat catccaagat gggaagatca tctttatgcc gaatggatat 5821 ataacacagt gtccaaatct aaatcacact tgcccaacct gcagtgattt cttaagcctg 5881 gtgcaaggaa taatggattt acaagagctt ttggccaaga tgactgcaaa actaaattat 5941 gcagagacaa gacttagtca attggaaaac tgtcattgtg agaagacttg tcaagtgagt 6001 ggactgctct atcgagatca agactcttgg gtagatggtg accattgcag gaactgcact 6061 tgcaaaagtg gtgccgtgga atgccgaagg atgtcctgtc cccctctcaa ttgctcccca 6121 gactccctcc cagtgcacat tgctggccag tgctgtaagg tctgccgacc aaaatgtatc 6181 tatggaggaa aagttcttgc agaaggccag cggattttaa ccaagagctg tcgggaatgc 6241 cgaggtggag ttttagtaaa aattacagaa atgtgtcctc ctttgaactg ctcagaaaag 6301 gatcacattc ttcctgagaa tcagtgctgc cgtgtctgta gaggtcataa cttttgtgca 6361 gaaggaccta aatgtggtga aaactcagag tgcaaaaact ggaatacaaa agctacttgt 6421 gagtgcaaga gtggttacat ctctgtccag ggagactctg cctactgtga agatattgat 6481 gagtgtgcag ctaagatgca ttactgtcat gccaatactg tgtgtgtcaa ccttcctggg 6541 ttatatcgct gtgactgtgt cccaggatac attcgtgtgg atgacttctc ttgtacagaa 6601 cacgatgaat gtggcagcgg ccagcacaac tgtgatgaga atgccatctg caccaacact 6661 gtccagggac acagctgcac ctgcaaaccg ggctacgtgg ggaacgggac catctgcaga 6721 gctttctgtg aagagggctg cagatacggt ggaacgtgtg tggctcccaa caaatgtgtc 6781 tgtccatctg gattcacagg aagccactgc gagaaagata ttgatgaatg ttcagaggga 6841 atcattgagt gccacaacca ttcccgctgc gttaacctgc cagggtggta ccactgtgag 6901 tgcagaagcg gtttccatga cgatgggacc tattcactgt ccggggagtc ctgtattgac 6961 attgatgaat gtgccttaag aactcacacc tgttggaacg attctgcctg catcaacctg 7021 gcagggggct ttgactgtct ctgcccctct gggccctcct gctctggtga ctgtcctcat 7081 gaaggggggc tgaagcacaa tggccaggtg tggaccttga aagaagacag gtgttctgtc 7141 tgctcctgca aggatggcaa gatattctgc cgacggacag cttgtgattg ccagaatcca 7201 agtgctgacc tattctgttg cccagaatgt gacaccagag tcacaagtca atgtttagac 7261 caaaatggtc acaagctgta tcgaagtgga gacaattgga cccatagctg tcagcagtgt 7321 cggtgtctgg aaggagaggt agattgctgg ccactcactt gccccaactt gagctgtgag 7381 tatacagcta tcttagaagg ggaatgttgt ccccgctgtg tcagtgaccc ctgcctagct 7441 gataacatca cctatgacat cagaaaaact tgcctggaca gctatggtgt ttcacggctt 7501 agtggctcag tgtggacgat ggctggatct ccctgcacaa cctgtaaatg caagaatgga 7561 agagtctgtt gttctgtgga ttttgagtgt cttcaaaata atctcgagca ccaccaccac 7621 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc 7681 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg 7741 ctgaaaggag gaactatatc cggat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.