pT7-6×His-ADAR1-6×His plasmid

pT7-6×His-ADAR1-6×His plasmid

PVT87146

pT7-6×His-ADAR1-6×His plasmid Information

Plasmid name: pT7-6×His-ADAR1(human)-6×His
Alias: -
Gene lenght: 3678bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pET-28a
Forward primer: T7
Reverse primer:
Copy number: High
Induction:

pT7-6×His-ADAR1-6×His plasmid Description

pT7-6×His-ADAR1-6×His This is a prokaryotic induction-type low-copy expression plasmid. It has a T7-LacO promoter system, which, under the induction of IPTG or lactose and its analogs, allows T7 RNA polymerase to initiate the transcription and translation of the target gene ADAR1. It carries His, Thrombin, and T7 protein tags at the N-terminus, and a His tag at the C-terminus, which facilitate subsequent detection, purification, and localization studies of the expressed protein. Additionally, the plasmid carries a kanamycin resistance gene, aiding in the selection of positive clones.

pT7-6×His-ADAR1-6×His plasmid Sequence

1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 5041 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac 5101 agcagcggcc tggtgccgcg cggcagccat atggctagca tgactggtgg acagcaaatg 5161 ggtcgcggat ccatgaatcc gcggcagggg tattccctca gcggatacta cacccatcca 5221 tttcaaggct atgagcacag acagctcaga taccagcagc ctgggccagg atcttccccc 5281 agtagtttcc tgcttaagca aatagaattt ctcaaggggc agctcccaga agcaccggtg 5341 attggaaagc agacaccgtc actgccacct tccctcccag gactccggcc aaggtttcca 5401 gtactacttg cctccagtac cagaggcagg caagtggaca tcaggggtgt ccccaggggc 5461 gtgcatctcg gaagtcaggg gctccagaga gggttccagc atccttcacc acgtggcagg 5521 agtctgccac agagaggtgt tgattgcctt tcctcacatt tccaggaact gagtatctac 5581 caagatcagg aacaaaggat cttaaagttc ctggaagagc ttggggaagg gaaggccacc 5641 acagcacatg atctgtctgg gaaacttggg actccgaaga aagaaatcaa tcgagtttta 5701 tactccctgg caaagaaggg caagctacag aaagaggcag gaacaccccc tttgtggaaa 5761 atcgcggtct ccactcaggc ttggaaccag cacagcggag tggtaagacc agacggtcat 5821 agccaaggag ccccaaactc agacccgagt ttggaaccgg aagacagaaa ctccacatct 5881 gtctcagaag atcttcttga gccttttatt gcagtctcag ctcaggcttg gaaccagcac 5941 agcggagtgg taagaccaga cagtcatagc caaggatccc caaactcaga cccaggtttg 6001 gaacctgaag acagcaactc cacatctgcc ttggaagatc ctcttgagtt tttagacatg 6061 gccgagatca aggagaaaat ctgcgactat ctcttcaatg tgtctgactc ctctgccctg 6121 aatttggcta aaaatattgg ccttaccaag gcccgagata taaatgctgt gctaattgac 6181 atggaaaggc agggggatgt ctatagacaa gggacaaccc ctcccatatg gcatttgaca 6241 gacaagaagc gagagaggat gcaaatcaag agaaatacga acagtgttcc tgaaaccgct 6301 ccagctgcaa tccctgagac caaaagaaac gcagagttcc tcacctgtaa tatacccaca 6361 tcaaatgcct caaataacat ggtaaccaca gaaaaagtgg agaatgggca ggaacctgtc 6421 ataaagttag aaaacaggca agaggccaga ccagaaccag caagactgaa accacctgtt 6481 cattacaatg gcccctcaaa agcagggtat gttgactttg aaaatggcca gtgggccaca 6541 gatgacatcc cagatgactt gaatagtatc cgcgcagcac caggtgagtt tcgagccatc 6601 atggagatgc cctccttcta cagtcatggc ttgccacggt gttcacccta caagaaactg 6661 acagagtgcc agctgaagaa ccccatcagc gggctgttag aatatgccca gttcgctagt 6721 caaacctgtg agttcaacat gatagagcag agtggaccac cccatgaacc tcgatttaaa 6781 ttccaggttg tcatcaatgg ccgagagttt cccccagctg aagctggaag caagaaagtg 6841 gccaagcagg atgcagctat gaaagccatg acaattctgc tagaggaagc caaagccaag 6901 gacagtggaa aatcagaaga atcatcccac tattccacag agaaagaatc agagaagact 6961 gcagagtccc agacccccac cccttcagcc acatccttct tttctgggaa gagccccgtc 7021 accacactgc ttgagtgtat gcacaaattg gggaactcct gcgaattccg tctcctgtcc 7081 aaagaaggcc ctgcccatga acccaagttc caatactgtg ttgcagtggg agcccaaact 7141 ttccccagtg tgagtgctcc cagcaagaaa gtggcaaagc agatggccgc agaggaagcc 7201 atgaaggccc tgcatgggga ggcgaccaac tccatggctt ctgataacca gcctgaaggt 7261 atgatctcag agtcacttga taacttggaa tccatgatgc ccaacaaggt caggaagatt 7321 ggcgagctcg tgagatacct gaacaccaac cctgtgggtg gccttttgga gtacgcccgc 7381 tcccatggct ttgctgctga attcaagttg gtcgaccagt ccggacctcc tcacgagccc 7441 aagttcgttt accaagcaaa agttgggggt cgctggttcc cagccgtctg cgcacacagc 7501 aagaagcaag gcaagcagga agcagcagat gcggctctcc gtgtcttgat tggggagaac 7561 gagaaggcag aacgcatggg tttcacagag gtaaccccag tgacaggggc cagtctcaga 7621 agaactatgc tcctcctctc aaggtcccca gaagcacagc caaagacact ccctctcact 7681 ggcagcacct tccatgacca gatagccatg ctgagccacc ggtgcttcaa cactctgact 7741 aacagcttcc agccctcctt gctcggccgc aagattctgg ccgccatcat tatgaaaaaa 7801 gactctgagg acatgggtgt cgtcgtcagc ttgggaacag ggaatcgctg tgtaaaagga 7861 gattctctca gcctaaaagg agaaactgtc aatgactgcc atgcagaaat aatctcccgg 7921 agaggcttca tcaggtttct ctacagtgag ttaatgaaat acaactccca gactgcgaag 7981 gatagtatat ttgaacctgc taagggagga gaaaagctcc aaataaaaaa gactgtgtca 8041 ttccatctgt atatcagcac tgctccgtgt ggagatggcg ccctctttga caagtcctgc 8101 agcgaccgtg ctatggaaag cacagaatcc cgccactacc ctgtcttcga gaatcccaaa 8161 caaggaaagc tccgcaccaa ggtggagaac ggagaaggca caatccctgt ggaatccagt 8221 gacattgtgc ctacgtggga tggcattcgg ctcggggaga gactccgtac catgtcctgt 8281 agtgacaaaa tcctacgctg gaacgtgctg ggcctgcaag gggcactgtt gacccacttc 8341 ctgcagccca tttatctcaa atctgtcaca ttgggttacc ttttcagcca agggcatctg 8401 acccgtgcta tttgctgtcg tgtgacaaga gatgggagtg catttgagga tggactacga 8461 catcccttta ttgtcaacca ccccaaggtt ggcagagtca gcatatatga ttccaaaagg 8521 caatccggga agactaagga gacaagcgtc aactggtgtc tggctgatgg ctatgacctg 8581 gagatcctgg acggtaccag aggcactgtg gatgggccac ggaatgaatt gtcccgggtc 8641 tccaaaaaga acatttttct tctatttaag aagctctgct ccttccgtta ccgcagggat 8701 ctactgagac tctcctatgg tgaggccaag aaagctgccc gtgactacga gacggccaag 8761 aactacttca aaaaaggcct gaaggatatg ggctatggga actggattag caaaccccag 8821 gaggaaaaga acttttatct ctgcccagta tagctcgagc accaccacca ccaccactga 8881 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa 8941 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga 9001 ggaactatat ccggat

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.