pCMV-Golgi-sfGFP-Neo plasmid

pCMV-Golgi-sfGFP-Neo plasmid

PVT87141

pCMV-Golgi-sfGFP-Neo plasmid Information

Plasmid name: pCMV-Golgi-sfGFP-Neo
Alias: -
Gene lenght:
Host: Mammalian cells
Use(s): subcellular localization
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: sfGFP-N1
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Golgi-sfGFP-Neo plasmid Description

pCMV-Golgi-sfGFP-Neo This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a strong CMV promoter that can initiate the transcription of the downstream gene. The plasmid carries a Golgi gene, allowing the target protein to enter the Golgi apparatus and perform specific functions. The plasmid carries an sfGFP green fluorescent protein for observing the expression and localization of the target gene. The plasmid carries a Neo/G418 resistance gene for selecting host cells that express the target gene.

pCMV-Golgi-sfGFP-Neo plasmid Sequence

1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatga 541 ggcttcggga gccgctcctg agcggcagcg ccgcgatgcc aggcgcgtcc ctacagcggg 601 cctgccgcct gctcgtggcc gtctgcgctc tgcaccttgg cgtcaccctc gtttactacc 661 tggctggccg cgacctgagc cgcctgcccc aactggtcgg agtctccaca ccgctgcagg 721 gcggctcgaa cagtgccgcc gccatcgggc agtcctccgg ggagctccgg accggagggg 781 ccaaggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg 841 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgcgcggcg 901 agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca 961 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca 1021 gccgctaccc cgaccacatg aagcgccacg acttcttcaa gtccgccatg cccgaaggct 1081 acgtccagga gcgcaccatc agcttcaagg acgacggcac ctacaagacc cgcgccgagg 1141 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 1201 aggacggcaa catcctgggg cacaagctgg agtacaactt caacagccac aacgtctata 1261 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacgtgg 1321 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 1381 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgtgctgagc aaagacccca 1441 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactcacg 1501 gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca 1561 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 1621 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 1681 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 1741 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa 1801 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 1861 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 1921 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 1981 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 2041 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 2101 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 2161 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 2221 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 2281 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 2341 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta 2401 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 2461 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 2521 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 2581 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 2641 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 2701 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga 2761 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 2821 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 2881 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga 2941 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 3001 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 3061 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 3121 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 3181 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 3241 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga 3301 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 3361 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 3421 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 3481 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 3541 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 3601 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 3661 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct 3721 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg 3781 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt 3841 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc 3901 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca 3961 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt 4021 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 4081 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 4141 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 4201 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 4261 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 4321 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 4381 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 4441 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 4501 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 4561 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 4621 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 4681 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 4741 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 4801 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcattag 4861 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.